Construct: sgRNA BRDN0001147712
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCCCGAGACAGTGTACCGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- GSK3A (2931)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76372
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
42236662 |
- |
GSK3A |
NNNCGAGACAGTGTACCGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000018.10 |
18 |
79973753 |
- |
TXNL4A |
NNNCGAGACGGTGTACCGCG |
NGG |
2 |
0.249 |
Tier I |
| 3 |
NC_000017.11 |
17 |
30496232 |
- |
GOSR1 |
NNNAGAGACAGTGTACCGGA |
NGG |
2 |
0.7895 |
Tier II |
| 4 |
NC_000011.10 |
11 |
11274131 |
+ |
GALNT18 |
NNNTGAGAGAGTGTACCGGG |
NGG |
2 |
0.4952 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
25233630 |
- |
Gsk3a |
NNNCGAGACGGTGTACCGAG |
NGG |
2 |
0.3968 |
Tier I |
| 2 |
NC_000069.6 |
3 |
142834254 |
- |
Pkn2 |
NNNAGAGAGAGTGTACCGGG |
NGG |
2 |
0.5213 |
Tier II |
| 3 |
NC_000068.7 |
2 |
93735811 |
+ |
Ext2 |
NNNCCAGACAGTGTGCCGGG |
NGG |
2 |
0.5107 |
Tier II |
| 4 |
NC_000078.6 |
12 |
31831437 |
+ |
Cog5 |
NNNCGAGACAGTGTACCGGA |
NGC |
2 |
0.0208 |
Tier II |
| 5 |
NC_000086.7 |
X |
142842630 |
- |
Tmem164 |
NNNCGAGACAGTGCACCGGG |
NGT |
2 |
0.0046 |
Tier II |
Other clones with same target sequence:
(none)
