Construct: sgRNA BRDN0001147714
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTTGGGTTGGTGTAATGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3C3 (5289)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76964
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000018.10 |
18 |
42004480 |
- |
PIK3C3 |
NNNTGGGTTGGTGTAATGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
227031329 |
+ |
CDC42BPA |
NNNTGGGTTGTTTTAATGAG |
NGG |
2 |
0.1154 |
Tier I |
| 3 |
NC_000003.12 |
3 |
159808719 |
- |
SCHIP1 |
NNNTGGGCTGGTATAATGAG |
NGG |
2 |
0.9231 |
Tier II |
| 4 |
NC_000003.12 |
3 |
159808719 |
- |
IQCJ-SCHIP1 |
NNNTGGGCTGGTATAATGAG |
NGG |
2 |
0.9231 |
Tier II |
| 5 |
NC_000003.12 |
3 |
27134278 |
+ |
NEK10 |
NNNTGGGATGGAGTAATGAG |
NGG |
2 |
0.64 |
Tier II |
| 6 |
NC_000005.10 |
5 |
15936083 |
- |
FBXL7 |
NNNTGGGATGGAGTAATGAG |
NGG |
2 |
0.64 |
Tier II |
| 7 |
NC_000010.11 |
10 |
124508726 |
- |
LHPP |
NNNTGGGCTGGTGAAATGAG |
NGG |
2 |
0.619 |
Tier II |
| 8 |
NC_000007.14 |
7 |
108164122 |
+ |
NRCAM |
NNNTGGGGTGGGGTAATGAG |
NGG |
2 |
0.3667 |
Tier II |
| 9 |
NC_000007.14 |
7 |
108164183 |
+ |
NRCAM |
NNNTGGGGTGGGGTAATGAG |
NGG |
2 |
0.3667 |
Tier II |
| 10 |
NC_000007.14 |
7 |
108164243 |
+ |
NRCAM |
NNNTGGGGTGGGGTAATGAG |
NGG |
2 |
0.3667 |
Tier II |
| 11 |
NC_000011.10 |
11 |
21457244 |
+ |
NELL1 |
NNNTGGGTTGGTGAAATGTG |
NGG |
2 |
0.3333 |
Tier II |
| 12 |
NC_000007.14 |
7 |
158015103 |
+ |
PTPRN2 |
NNNTGGGTTGCTGAAATGAG |
NGG |
2 |
0.2653 |
Tier II |
| 13 |
NC_000012.12 |
12 |
45264102 |
+ |
ANO6 |
NNNTTGGTGGGTGTAATGAG |
NGG |
2 |
0.1857 |
Tier II |
| 14 |
NC_000002.12 |
2 |
47583953 |
+ |
MSH2 |
NNNTGTGTTGGTGTCATGAG |
NGG |
2 |
0.1818 |
Tier II |
| 15 |
NC_000007.14 |
7 |
29342491 |
+ |
CHN2 |
NNNTGGGTTGCTTTAATGAG |
NGG |
2 |
0.1286 |
Tier II |
| 16 |
NC_000016.10 |
16 |
88585952 |
+ |
ZC3H18 |
NNNTGGGGTGGTGTAAGGAG |
NGG |
2 |
0.0863 |
Tier II |
| 17 |
NC_000004.12 |
4 |
10630882 |
- |
CLNK |
NNNTGGCTTGGTGTAATGAG |
NGA |
2 |
0.0477 |
Tier II |
| 18 |
NC_000011.10 |
11 |
84863535 |
- |
DLG2 |
NNNTGTGTTGGTGTAATGAG |
NGA |
2 |
0.0463 |
Tier II |
| 19 |
NC_000003.12 |
3 |
159808719 |
- |
LOC101928351 |
NNNTGGGCTGGTATAATGAG |
NGG |
2 |
0.9231 |
Tier III |
| 20 |
NC_000005.10 |
5 |
16377940 |
- |
LINC02150 |
NNNTGGGTTGGTGTGGTGAG |
NGG |
2 |
0.125 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
30298697 |
- |
Pik3c3 |
NNNGGGGTTGGTGTAATGAG |
NGG |
1 |
0.625 |
Tier I |
| 2 |
NC_000068.7 |
2 |
146061930 |
- |
Cfap61 |
NNNTGGGTTGGTGTAAAGAG |
NGG |
1 |
0.5333 |
Tier II |
| 3 |
NC_000068.7 |
2 |
60452656 |
+ |
Pla2r1 |
NNNTGGGTTTGTGAAATGAG |
NGG |
2 |
0.503 |
Tier II |
| 4 |
NC_000077.6 |
11 |
54113537 |
+ |
P4ha2 |
NNNTGGCTTGGTGTGATGAG |
NGG |
2 |
0.4469 |
Tier II |
| 5 |
NC_000075.6 |
9 |
58669798 |
- |
Rec114 |
NNNGGGGTGGGTGTAATGAG |
NGG |
2 |
0.3869 |
Tier II |
| 6 |
NC_000081.6 |
15 |
18826677 |
+ |
Cdh10 |
NNNTGGTTTGGTGTAAAGAG |
NGG |
2 |
0.3048 |
Tier II |
| 7 |
NC_000073.6 |
7 |
49209156 |
- |
Nav2 |
NNNTGGGTTGCTGAAATGAG |
NGG |
2 |
0.2653 |
Tier II |
| 8 |
NC_000075.6 |
9 |
102185652 |
+ |
Ephb1 |
NNNTGGGTTGGTGAAATGGG |
NGG |
2 |
0.2321 |
Tier II |
| 9 |
NC_000071.6 |
5 |
138991596 |
+ |
Pdgfa |
NNNTGGGTTGGTGTCATGAT |
NGG |
2 |
0.1909 |
Tier II |
| 10 |
NC_000073.6 |
7 |
24403173 |
+ |
Smg9 |
NNNTGGGTGGGTGTAATGAG |
NGC |
2 |
0.0138 |
Tier II |
| 11 |
NC_000071.6 |
5 |
119684529 |
+ |
Tbx3 |
NNNTGGATTGGTGTACTGAG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000077.6 |
11 |
73093181 |
- |
Itgae |
NNNTGGGTTGGTGTACTGAG |
NAG |
2 |
0.0 |
Tier II |
| 13 |
NC_000073.6 |
7 |
78584183 |
- |
E430016F16Rik |
NNNTGGGTTGGATTAATGAG |
NGG |
2 |
0.24 |
Tier III |
| 14 |
NC_000070.6 |
4 |
117504830 |
- |
Gm12827 |
NNNTGGGTTTGTGTAAGGAG |
NGG |
2 |
0.0956 |
Tier III |
Other clones with same target sequence:
(none)
