Construct: sgRNA BRDN0001147715
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTTGGAGGCTTCTTTATCCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PANK4 (55229)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76969
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
2519201 |
- |
PANK4 |
NNNGGAGGCTTCTTTATCCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
75141285 |
+ |
KCNC2 |
NNNTGAGGCTTCTTTATCCT |
NGG |
2 |
0.2545 |
Tier II |
| 3 |
NC_000011.10 |
11 |
62835397 |
+ |
WDR74 |
NNNGGAGGCTGCTTTATCCC |
NGG |
2 |
0.1714 |
Tier II |
| 4 |
NC_000001.11 |
1 |
44326309 |
- |
ERI3 |
NNNGGAGGCTTCTTGATCCT |
NGG |
2 |
0.035 |
Tier II |
| 5 |
NC_000008.11 |
8 |
124998855 |
- |
SQLE |
NNNAGAGGCTTCTTTTTCCG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000004.12 |
4 |
73527032 |
+ |
LINC02499 |
NNNGGAGGCTTCTTTATAGG |
NGG |
2 |
0.0673 |
Tier III |
| 7 |
NC_000006.12 |
6 |
6712896 |
+ |
LOC101928004 |
NNNGGAGGCTTCTGTATCCA |
NGG |
2 |
0.0 |
Tier III |
| 8 |
NC_000006.12 |
6 |
6712896 |
+ |
LOC101928047 |
NNNGGAGGCTTCTGTATCCA |
NGG |
2 |
0.0 |
Tier III |
| 9 |
NC_000006.12 |
6 |
7675237 |
+ |
LOC105374906 |
NNNGGAGGCTTCTGTATGCG |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
154971497 |
+ |
Pank4 |
NNNGGGGGCTTCTTCATCCG |
NGG |
2 |
0.1948 |
Tier I |
| 2 |
NC_000085.6 |
19 |
34120436 |
- |
Lipm |
NNNGGAGTCTTCTTTATCCA |
NGG |
2 |
0.5859 |
Tier II |
| 3 |
NC_000067.6 |
1 |
158942220 |
+ |
Pappa2 |
NNNGGAGGCTTCTTCATCCA |
NGG |
2 |
0.2557 |
Tier II |
| 4 |
NC_000073.6 |
7 |
78331094 |
+ |
Ntrk3 |
NNNGGAGGCTTCTCTATCTG |
NGG |
2 |
0.1319 |
Tier II |
| 5 |
NC_000076.6 |
10 |
107313499 |
+ |
Lin7a |
NNNGGAGGCTCCTTTCTCCG |
NGG |
2 |
0.0 |
Tier II |
| 6 |
NC_000070.6 |
4 |
98426864 |
+ |
Patj |
NNNGGAGGCTTCTTTCTCCG |
NGA |
2 |
0.0 |
Tier II |
| 7 |
NC_000074.6 |
8 |
9543047 |
+ |
Fam155a |
NNNGGAGGCTTCTTTCTCCG |
NGA |
2 |
0.0 |
Tier II |
| 8 |
NC_000069.6 |
3 |
62660710 |
- |
Gm34048 |
NNNGGAGGCTTCTTTCTCTG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
