Construct: sgRNA BRDN0001147716
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTAGTAGCGAGGTATGAGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PTK7 (5754)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 78021
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
43130568 |
+ |
PTK7 |
NNNAGTAGCGAGGTATGAGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
63838319 |
- |
SMARCD2 |
NNNAGTAGGGAGGTAAGAGG |
NGG |
2 |
0.5628 |
Tier II |
| 3 |
NC_000010.11 |
10 |
92512315 |
- |
IDE |
NNNAGTATAGAGGTATGAGG |
NGG |
2 |
0.5357 |
Tier II |
| 4 |
NC_000001.11 |
1 |
214427735 |
+ |
PTPN14 |
NNNAGTAGCAAGGTAGGAGG |
NGG |
2 |
0.3231 |
Tier II |
| 5 |
NC_000022.11 |
22 |
50423344 |
- |
PPP6R2 |
NNNGGTAGCGAGGTATGTGG |
NGG |
2 |
0.3125 |
Tier II |
| 6 |
NC_000004.12 |
4 |
36341243 |
+ |
DTHD1 |
NNNAGTAGCAAGGGATGAGG |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000016.10 |
16 |
76327721 |
+ |
CNTNAP4 |
NNNAGTAGTGAGGGATGAGG |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000001.11 |
1 |
98179576 |
- |
LOC124900404 |
NNNAGTGGCTAGGTATGAGG |
NGG |
2 |
0.5735 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000084.6 |
18 |
37864567 |
+ |
Diaph1 |
NNNAGAAGTGAGGTATGAGG |
NGG |
2 |
0.7583 |
Tier II |
| 2 |
NC_000081.6 |
15 |
78691481 |
- |
Elfn2 |
NNNAGGGGCGAGGTATGAGG |
NGG |
2 |
0.4034 |
Tier II |
| 3 |
NC_000080.6 |
14 |
32428031 |
+ |
Chat |
NNNAGTAGAGAGGTAGGAGG |
NGG |
2 |
0.2967 |
Tier II |
| 4 |
NC_000079.6 |
13 |
56106192 |
+ |
Macroh2a1 |
NNNAGTTGGGAGGTATGAGG |
NGG |
2 |
0.2708 |
Tier II |
| 5 |
NC_000077.6 |
11 |
33121241 |
- |
Fgf18 |
NNNAGTAGGGAGGTAGGAGG |
NGG |
2 |
0.2143 |
Tier II |
| 6 |
NC_000069.6 |
3 |
79141265 |
- |
Rapgef2 |
NNNAGTAGCGAGGCAGGAGG |
NGG |
2 |
0.0989 |
Tier II |
| 7 |
NC_000075.6 |
9 |
54543654 |
- |
Sh2d7 |
NNNAGTATCGAGGTATGAGG |
NGA |
2 |
0.0434 |
Tier II |
| 8 |
NC_000081.6 |
15 |
62134279 |
+ |
Pvt1 |
NNNAGGAGAGAGGTATGAGG |
NGG |
2 |
0.4898 |
Tier III |
| 9 |
NC_000081.6 |
15 |
78691481 |
- |
Gm52188 |
NNNAGGGGCGAGGTATGAGG |
NGG |
2 |
0.4034 |
Tier III |
Other clones with same target sequence:
(none)
