Construct: sgRNA BRDN0001147718
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCCCATCCCCTACCAGCGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP2K2 (5605)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77601
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
4123799 |
- |
MAP2K2 |
NNNCCATCCCCTACCAGCGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
87506082 |
+ |
SPARCL1 |
NNNCCATCCCCCACCAGCTA |
NGG |
2 |
0.6316 |
Tier II |
| 3 |
NC_000016.10 |
16 |
87735409 |
+ |
KLHDC4 |
NNNCCGTCCCCAACCAGCGA |
NGG |
2 |
0.5714 |
Tier II |
| 4 |
NC_000015.10 |
15 |
63929632 |
+ |
DAPK2 |
NNNCCATCCCCGACCAGCAA |
NGG |
2 |
0.3571 |
Tier II |
| 5 |
NC_000016.10 |
16 |
85415571 |
+ |
GSE1 |
NNNCCGTCCCCTACCAGCCA |
NGG |
2 |
0.3202 |
Tier II |
| 6 |
NC_000023.11 |
X |
139117996 |
- |
FGF13 |
NNNCCATCCCCTACCAGCCT |
NGG |
2 |
0.269 |
Tier II |
| 7 |
NC_000007.14 |
7 |
38430573 |
+ |
AMPH |
NNNCCATACCCTCCCAGCGA |
NGG |
2 |
0.1368 |
Tier II |
| 8 |
NC_000017.11 |
17 |
60934634 |
- |
BCAS3 |
NNNCCATCCCCCACCAGGGA |
NGG |
2 |
0.1263 |
Tier II |
| 9 |
NC_000016.10 |
16 |
25138089 |
+ |
LCMT1 |
NNNCCATCCTCTACCAGCGA |
NGC |
2 |
0.0209 |
Tier II |
| 10 |
NC_000007.14 |
7 |
129126595 |
+ |
LOC407835 |
NNNCCGTCCCCAACCAGCGA |
NGG |
2 |
0.5714 |
Tier III |
| 11 |
NC_000016.10 |
16 |
87735409 |
+ |
LOC124903752 |
NNNCCGTCCCCAACCAGCGA |
NGG |
2 |
0.5714 |
Tier III |
| 12 |
NC_000015.10 |
15 |
63929632 |
+ |
LOC101928988 |
NNNCCATCCCCGACCAGCAA |
NGG |
2 |
0.3571 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
81106224 |
+ |
Map2k2 |
NNNCCGTCCCCCACCAGCGA |
NGG |
2 |
0.6767 |
Tier I |
| 2 |
NC_000071.6 |
5 |
124124881 |
- |
Pitpnm2 |
NNNCCATCCCCTACCACCCA |
NGG |
2 |
0.1055 |
Tier I |
| 3 |
NC_000072.6 |
6 |
27716480 |
- |
Grm8 |
NNNCCATCTCCTACCAGCAA |
NGG |
2 |
0.625 |
Tier II |
| 4 |
NC_000081.6 |
15 |
88859970 |
- |
Gm33049 |
NNNCCACCCCCTACCAGCAA |
NGG |
2 |
0.4911 |
Tier II |
| 5 |
NC_000077.6 |
11 |
88113593 |
+ |
Cuedc1 |
NNNCCATCCCCTGCCAGTGA |
NGG |
2 |
0.4193 |
Tier II |
| 6 |
NC_000078.6 |
12 |
112063180 |
+ |
Tdrd9 |
NNNCCATCCCATACCAGAGA |
NGG |
2 |
0.4038 |
Tier II |
| 7 |
NC_000076.6 |
10 |
92687862 |
- |
Nedd1 |
NNNCAATCCCCTACCAGGGA |
NGG |
2 |
0.0762 |
Tier II |
Other clones with same target sequence:
(none)
