Construct: sgRNA BRDN0001147726
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGCTCGAAAGTGCGCATGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- OBSCN (84033)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76115
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
228264275 |
+ |
OBSCN |
NNNCTCGAAAGTGCGAATGG |
NGG |
1 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
228256810 |
+ |
OBSCN |
NNNCTCGAAAGTGCGCATGG |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000001.11 |
1 |
228273431 |
+ |
OBSCN |
NNNCTCAAAAGTGCACATGG |
NGG |
2 |
0.9412 |
Tier I |
| 4 |
NC_000001.11 |
1 |
228245572 |
+ |
OBSCN |
NNNCTCAAAAGTACGCATGG |
NGG |
2 |
0.9231 |
Tier I |
| 5 |
NC_000001.11 |
1 |
228224618 |
+ |
OBSCN |
NNNCTCGAAAGTGTGCATGG |
NGG |
1 |
0.7333 |
Tier I |
| 6 |
NC_000001.11 |
1 |
228246687 |
+ |
OBSCN |
NNNTTCGAAAGTGCGCATAG |
NGG |
2 |
0.5714 |
Tier I |
| 7 |
NC_000001.11 |
1 |
228250124 |
+ |
OBSCN |
NNNCTCGAAAGTTCGAATGG |
NGG |
2 |
0.3 |
Tier I |
| 8 |
NC_000010.11 |
10 |
93066266 |
- |
CYP26C1 |
NNNCTCGAAGGTGCGCAGGG |
NGG |
2 |
0.1852 |
Tier I |
| 9 |
NC_000001.11 |
1 |
228268689 |
+ |
OBSCN |
NNNCTCGAAAGTGCGCGTGG |
NGG |
1 |
0.1765 |
Tier I |
| 10 |
NC_000001.11 |
1 |
228219483 |
+ |
OBSCN |
NNNCTCGAAAGTGCACGTGG |
NGG |
2 |
0.1661 |
Tier I |
| 11 |
NC_000001.11 |
1 |
228244455 |
+ |
OBSCN |
NNNCTCAAAAGTGGGCATGG |
NGG |
2 |
0.0 |
Tier I |
| 12 |
NC_000013.11 |
13 |
60482829 |
+ |
TDRD3 |
NNNCTTGAAAGTGCACATGG |
NGG |
2 |
0.8739 |
Tier II |
| 13 |
NC_000020.11 |
20 |
23421017 |
+ |
NAPB |
NNNCTCTAAAGTGCGCGTGG |
NGG |
2 |
0.1008 |
Tier II |
| 14 |
NC_000020.11 |
20 |
3238794 |
- |
SLC4A11 |
NNNCTCGAAAGTGGGCAGGG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000001.11 |
1 |
228273431 |
+ |
LOC124904535 |
NNNCTCAAAAGTGCACATGG |
NGG |
2 |
0.9412 |
Tier III |
| 16 |
NC_000015.10 |
15 |
95288308 |
- |
LETR1 |
NNNATCGAAAGTGCGCATGG |
NAG |
2 |
0.2183 |
Tier III |
| 17 |
NC_000001.11 |
1 |
228268689 |
+ |
LOC124904535 |
NNNCTCGAAAGTGCGCGTGG |
NGG |
1 |
0.1765 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
59112636 |
- |
Obscn |
NNNCTCAAAGGTGCGCATGG |
NGG |
2 |
0.5556 |
Tier I |
| 2 |
NC_000077.6 |
11 |
59115776 |
- |
Obscn |
NNNCTCAAAGGTGCGCATGG |
NGG |
2 |
0.5556 |
Tier I |
| 3 |
NC_000077.6 |
11 |
59122737 |
- |
Obscn |
NNNCTCGAAGGTGCGCATGG |
NGG |
1 |
0.5556 |
Tier I |
| 4 |
NC_000077.6 |
11 |
59124638 |
- |
Obscn |
NNNCTCGAAGGTGCGCATGG |
NGG |
1 |
0.5556 |
Tier I |
| 5 |
NC_000077.6 |
11 |
59128180 |
- |
Obscn |
NNNCTCGAAGGTGCGCATGG |
NGG |
1 |
0.5556 |
Tier I |
| 6 |
NC_000077.6 |
11 |
59129687 |
- |
Obscn |
NNNCTCGAAGGTGCGCATGG |
NGG |
1 |
0.5556 |
Tier I |
| 7 |
NC_000077.6 |
11 |
59085182 |
- |
Obscn |
NNNCTCGAAGGTGCGCGTGG |
NGG |
2 |
0.098 |
Tier I |
| 8 |
NC_000077.6 |
11 |
59086782 |
- |
Obscn |
NNNCTCGAAGGTGCGCGTGG |
NGG |
2 |
0.098 |
Tier I |
| 9 |
NC_000077.6 |
11 |
59090625 |
- |
Obscn |
NNNCTCGAAGGTGCGCGTGG |
NGG |
2 |
0.098 |
Tier I |
| 10 |
NC_000077.6 |
11 |
59093413 |
- |
Obscn |
NNNCTCGAAGGTGCGCGTGG |
NGG |
2 |
0.098 |
Tier I |
| 11 |
NC_000077.6 |
11 |
59099807 |
- |
Obscn |
NNNCTCGAAGGTGCGCGTGG |
NGG |
2 |
0.098 |
Tier I |
| 12 |
NC_000077.6 |
11 |
59103392 |
- |
Obscn |
NNNCTCGAAGGTGCGCGTGG |
NGG |
2 |
0.098 |
Tier I |
| 13 |
NC_000077.6 |
11 |
59106412 |
- |
Obscn |
NNNCTCGAAGGTGCGCGTGG |
NGG |
2 |
0.098 |
Tier I |
| 14 |
NC_000076.6 |
10 |
123151932 |
- |
Usp15 |
NNNCTCCAAAGTGCGCATGC |
NGG |
2 |
0.2946 |
Tier II |
| 15 |
NC_000071.6 |
5 |
16010526 |
+ |
Cacna2d1 |
NNNCTCGAAAGTGCTCATGG |
NAG |
2 |
0.037 |
Tier II |
| 16 |
NC_000073.6 |
7 |
3406279 |
- |
Cacng8 |
NNNCTCCAAAGTGGGCATGG |
NGG |
2 |
0.0 |
Tier II |
Other clones with same target sequence:
(none)
