Construct: sgRNA BRDN0001147728

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGAGTGTCATGGACATGCCC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SCYL3 (57147)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76793

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 169870318 - SCYL3 NNNGTGTCATGGACATGCCC NGG 0 1.0 Tier I
2 NC_000001.11 1 92883019 + DIPK1A NNNGTGTCACAGACATGCCC NGG 2 0.5333 Tier II
3 NC_000010.11 10 25309671 + GPR158 NNNGTGTCATGGGCATGACC NGG 2 0.3512 Tier II
4 NC_000010.11 10 76295616 + LRMDA NNNGTGTCACGGACATGCAC NGG 2 0.2286 Tier II
5 NC_000022.11 22 44888801 + PHF21B NNNGTGTCATGCAAATGCCC NGG 2 0.1853 Tier II
6 NC_000007.14 7 1857381 + MAD1L1 NNNGTGTCCTGGTCATGCCC NGG 2 0.1714 Tier II
7 NC_000015.10 15 59035197 - RNF111 NNNGTGTCATGGACAGGGCC NGG 2 0.0462 Tier II
8 NC_000020.11 20 20275031 - CFAP61 NNNGTGTCATGGTCATGCCC NTG 2 0.0117 Tier II
9 NC_000009.12 9 113109395 + FAM225B NNNGTGCCATGGACAAGCCC NGG 2 0.625 Tier III
10 NC_000009.12 9 113115179 - FAM225A NNNGTGCCATGGACAAGCCC NGG 2 0.625 Tier III
11 NC_000018.10 18 22542256 + LOC124904265 NNNAAGTCATGGACATGCCC NGG 2 0.45 Tier III
12 NC_000011.10 11 5929004 + LOC112268071 NNNGAGTCATGCACATGCCC NGG 2 0.2647 Tier III
13 NC_000013.11 13 112451918 + LOC105370372 NNNGTGCCATGGACAGGCCC NGG 2 0.238 Tier III
14 NC_000010.11 10 76295616 + LOC124902462 NNNGTGTCACGGACATGCAC NGG 2 0.2286 Tier III
15 NC_000017.11 17 20695120 - LOC100287072 NNNTTGTCATGGACATGCCA NGG 2 0.1818 Tier III
16 NC_000022.11 22 22293226 - IGL NNNGTGTCATGGCCCTGCCC NGG 2 0.0574 Tier III
17 NC_000022.11 22 22293226 - LOC105372952 NNNGTGTCATGGCCCTGCCC NGG 2 0.0574 Tier III
18 NC_000001.11 1 69233657 - LINC02791 NNNGTGTCATGGAAATGCCC NTG 2 0.0136 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 107603528 - Rpap2 NNNGTGTGATGAACATGCCC NGG 2 0.6 Tier I
2 NC_000069.6 3 25137199 - Naaladl2 NNNGTGTCAAGGACAAGCCC NGG 2 0.7792 Tier II
3 NC_000073.6 7 45319587 + Trpm4 NNNGGGTCATGAACATGCCC NGG 2 0.5973 Tier II
4 NC_000076.6 10 110120822 + Nav3 NNNGTATCATGGACATGCAC NGG 2 0.4286 Tier II
5 NC_000075.6 9 95181535 - Slc9a9 NNNGTGTCATGGACATGCAC NGG 1 0.4286 Tier II
6 NC_000081.6 15 101254694 + Nr4a1 NNNGTGTAATGGGCATGCCC NGG 2 0.4239 Tier II
7 NC_000067.6 1 134690102 + Syt2 NNNGTGTCTTGGGCATGCCC NGG 2 0.3913 Tier II
8 NC_000068.7 2 96497192 + Lrrc4c NNNGTTTCATGCACATGCCC NGG 2 0.3529 Tier II
9 NC_000073.6 7 64156417 - Trpm1 NNNGTGTCATGATCATGCCC NGG 2 0.28 Tier II
10 NC_000075.6 9 108175247 - Bsn NNNGTTTCATGGACAGGCCC NGG 2 0.2308 Tier II
11 NC_000075.6 9 28120692 + Opcml NNNGTGTCATGGACAGGCCA NGG 2 0.1731 Tier II
12 NC_000076.6 10 20690402 + Pde7b NNNGTGCCATGGACATCCCC NGG 2 0.1618 Tier II
13 NC_000068.7 2 60494490 - Pla2r1 NNNGTGTCATGGCAATGCCC NGG 2 0.0737 Tier II
14 NC_000073.6 7 37685797 - Zfp536 NNNGAGTCATGGACATGCCC NGA 2 0.0347 Tier II
15 NC_000068.7 2 97338606 - Lrrc4c NNNGTGTCATGGACATGCTC NGA 2 0.0321 Tier II
16 NC_000069.6 3 114067280 + Col11a1 NNNGTGTCATGCACATGCCC NTG 2 0.0206 Tier II
17 NC_000085.6 19 24688770 - Pgm5 NNNGTGTCATGGAAATGCCC NGT 2 0.0056 Tier II
18 NC_000072.6 6 118826541 + Cacna1c NNNGTCTCATGGAGATGCCC NGG 2 0.0 Tier II
19 NC_000075.6 9 47351126 - Gm31816 NNNGGGTCATGGAAATGCCC NGG 2 0.224 Tier III
20 NC_000079.6 13 117132340 - Gm6416 NNNGTGTCATGCACCTGCCC NGG 2 0.1444 Tier III
21 NC_000070.6 4 114796727 + Gm33655 NNNGTGTCATAGACATGCCG NGG 2 0.0588 Tier III
Download CSV

Other clones with same target sequence:

(none)