Construct: sgRNA BRDN0001147733

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AGTGTAAAGCAACACACCCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NUAK1 (9891)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75576

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000012.12 12 106070888 + NUAK1 NNNGTAAAGCAACACACCCA NGG 0 1.0 Tier I
2 NC_000017.11 17 28611425 + RSKR NNNGAAAAGCAAGACACCCA NGG 2 0.0682 Tier I
3 NC_000005.10 5 169294367 + SLIT3 NNNACAAAGCAACACACCCA NGG 2 0.9 Tier II
4 NC_000003.12 3 23886318 - UBE2E1 NNNGTAGAGCAACACACCCA NGG 1 0.7059 Tier II
5 NC_000007.14 7 86852063 + GRM3 NNNGTAAAGAAACTCACCCA NGG 2 0.4622 Tier II
6 NC_000005.10 5 31641845 - PDZD2 NNNCTAAAGAAACACACCCA NGG 2 0.4588 Tier II
7 NC_000001.11 1 21313706 - ECE1 NNNGTAAGGCAACTCACCCA NGG 2 0.3911 Tier II
8 NC_000003.12 3 187284755 - MASP1 NNNGTTAAGCAACTCACCCA NGG 2 0.381 Tier II
9 NC_000014.9 14 68361284 + RAD51B NNNGTTAAGCAACTCACCCA NGG 2 0.381 Tier II
10 NC_000007.14 7 137416923 + DGKI NNNGTCAAGCAATACACCCA NGG 2 0.3182 Tier II
11 NC_000007.14 7 96500206 + SEM1 NNNGTGAAGCCACACACCCA NGG 2 0.2857 Tier II
12 NC_000009.12 9 122143520 + NDUFA8 NNNGTTAAGGAACACACCCA NGG 2 0.2778 Tier II
13 NC_000006.12 6 84090060 - MRAP2 NNNGTAAAGCAACTCACCAA NGG 2 0.2286 Tier II
14 NC_000016.10 16 13304769 + SHISA9 NNNGTTAAGCAACACACCCA NAG 2 0.1852 Tier II
15 NC_000016.10 16 15559487 - BMERB1 NNNGTTAAGCAACACACCCA NAG 2 0.1852 Tier II
16 NC_000016.10 16 15559487 - MPV17L-BMERB1 NNNGTTAAGCAACACACCCA NAG 2 0.1852 Tier II
17 NC_000005.10 5 146633782 - PPP2R2B NNNGTCAAGCCACACACCCA NGG 2 0.1818 Tier II
18 NC_000014.9 14 99195625 + BCL11B NNNGTAAAGCAACCCACCCG NGG 2 0.1639 Tier II
19 NC_000012.12 12 112998095 + OAS2 NNNGTAAAGCAGCAAACCCA NGG 2 0.1605 Tier II
20 NC_000014.9 14 78808398 - NRXN3 NNNCTAAAGCAACAAACCCA NGG 2 0.1176 Tier II
21 NC_000022.11 22 18092228 + PEX26 NNNGTCAAGCAACCCACCCA NGG 2 0.0974 Tier II
22 NC_000013.11 13 98174862 + FARP1 NNNGTAAAGTAACATACCCA NGG 2 0.0627 Tier II
23 NC_000011.10 11 61812902 - FADS1 NNNGTTAAGCAACACAGCCA NGG 2 0.042 Tier II
24 NC_000004.12 4 143135663 - USP38-DT NNNGTAAAAAAACACACCCA NGG 2 0.5571 Tier III
25 NC_000003.12 3 189467884 - LOC105374270 NNNGTTAAGCAATACACCCA NGG 2 0.5 Tier III
26 NC_000017.11 17 72604050 + LINC00511 NNNGGAAAGCAACTCACCCA NGG 2 0.3413 Tier III
27 NC_000007.14 7 96500206 + LOC105375411 NNNGTGAAGCCACACACCCA NGG 2 0.2857 Tier III
28 NC_000018.10 18 10384854 - LOC105371989 NNNGTAAGGGAACACACCCA NGG 2 0.2852 Tier III
29 NC_000007.14 7 26518350 - LINC02981 NNNGTGAAGGAACACACCCA NGG 2 0.2778 Tier III
30 NC_000010.11 10 119004270 - LINC03036 NNNGGAATGCAACACACCCA NGG 2 0.2743 Tier III
31 NC_000016.10 16 13304769 + LOC107984137 NNNGTTAAGCAACACACCCA NAG 2 0.1852 Tier III
32 NC_000017.11 17 28611425 + SPAG5-AS1 NNNGAAAAGCAAGACACCCA NGG 2 0.0682 Tier III
33 NC_000006.12 6 163942125 + LOC105378102 NNNGTAAAGCAACAAGCCCA NGG 2 0.0427 Tier III
34 NC_000002.12 2 52399663 - NRXN1-DT NNNGTAAAGCAACACATCCA NGA 2 0.0324 Tier III
35 NC_000012.12 12 27148965 + LOC124902904 NNNGTAAAGCAACTCACCCA NGT 2 0.0086 Tier III
36 NC_000021.9 21 16290772 + MIR99AHG NNNGTAAAGCAACACAGCCA NGC 2 0.0013 Tier III
37 NC_000014.9 14 63662792 - LOC105370532 NNNGAAAAGCAACACCCCCA NGG 2 0.0 Tier III
38 NC_000021.9 21 28018103 - LINC00314 NNNGAAAAGCAACACCCCCA NGG 2 0.0 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000078.6 12 73759602 - Prkch NNNATAAAGCAACACACCCA NGG 1 0.9 Tier I
2 NC_000077.6 11 78293933 - Rskr NNNGAAAAGCAACACACCCA NGG 1 0.5 Tier I
3 NC_000076.6 10 84378206 + Nuak1 NNNGTAAAGCAAGACACCCA NGG 1 0.1364 Tier I
4 NC_000081.6 15 34115230 - Mtdh NNNGCAAAGCAACACACACA NGG 2 0.5385 Tier II
5 NC_000075.6 9 80437286 - Impg1 NNNCTAAAGCAACACACCCA NGG 1 0.5294 Tier II
6 NC_000086.7 X 79499548 + Cfap47 NNNGTGGAGCAACACACCCA NGG 2 0.5042 Tier II
7 NC_000078.6 12 98247314 + Galc NNNGGAAAGCAAAACACCCA NGG 2 0.2462 Tier II
8 NC_000068.7 2 29670592 + Rapgef1 NNNGTACAGCAACACACACA NGG 2 0.2356 Tier II
9 NC_000073.6 7 86738720 + Folh1 NNNGAAAAGCAAAACACCCA NGG 2 0.1923 Tier II
10 NC_000068.7 2 139575551 - Sptlc3 NNNGTAAAGCCACACACCTA NGG 2 0.1846 Tier II
11 NC_000072.6 6 127627244 - Cracr2a NNNGTATAGGAACACACCCA NGG 2 0.1701 Tier II
12 NC_000081.6 15 101183909 + Acvr1b NNNGTAAATCTACACACCCA NGG 2 0.1641 Tier II
13 NC_000069.6 3 40701343 + Intu NNNGCAAAGCAACACACCGA NGG 2 0.125 Tier II
14 NC_000073.6 7 55175630 - Luzp2 NNNGTAAATCAACAAACCCA NGG 2 0.1185 Tier II
15 NC_000070.6 4 99889988 - Efcab7 NNNGTAAACCAAGACACCCA NGG 2 0.0734 Tier II
16 NC_000073.6 7 16201012 - Dhx34 NNNGAAAAGCAACACAGCCA NGG 2 0.0294 Tier II
17 NC_000077.6 11 78293933 - Gm3948 NNNGAAAAGCAACACACCCA NGG 1 0.5 Tier III
18 NC_000086.7 X 129058665 - 4930558G05Rik NNNATAAACCAACACACCCA NGG 2 0.4846 Tier III
19 NC_000071.6 5 23852654 - 2700038G22Rik NNNATAAAGCAACACACCCA NGT 2 0.0145 Tier III
20 NC_000071.6 5 23852654 - Gm42228 NNNATAAAGCAACACACCCA NGT 2 0.0145 Tier III
Download CSV

Other clones with same target sequence:

(none)