Construct: sgRNA BRDN0001147740
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATTCCACACAGGGTTTAGCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKCG (5582)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76898
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
53891800 |
- |
PRKCG |
NNNCCACACAGGGTTTAGCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000008.11 |
8 |
30555890 |
+ |
RBPMS |
NNNCCACACAGTGATTAGCG |
NGG |
2 |
0.2381 |
Tier I |
| 3 |
NC_000008.11 |
8 |
130441049 |
+ |
ASAP1 |
NNNCCACACAGGGCTTAGCA |
NGG |
2 |
0.2679 |
Tier II |
| 4 |
NC_000008.11 |
8 |
142495743 |
- |
ADGRB1 |
NNNCCACACTGGGTTTGGCG |
NGG |
2 |
0.1557 |
Tier II |
| 5 |
NC_000019.10 |
19 |
40318412 |
- |
C19orf47 |
NNNCCACACAGGGTTTAGCT |
NTG |
2 |
0.0273 |
Tier II |
| 6 |
NC_000008.11 |
8 |
68190105 |
+ |
PREX2 |
NNNCCACACAGGGTATAGCG |
NTG |
2 |
0.0226 |
Tier II |
| 7 |
NC_000014.9 |
14 |
95274612 |
- |
CLMN |
NNNCCACACAGGGTTTAGAG |
NTG |
2 |
0.0167 |
Tier II |
| 8 |
NC_000014.9 |
14 |
22334928 |
- |
TRA |
NNNCCACAGAGGGTTTAGGG |
NGG |
2 |
0.0774 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
96118132 |
- |
Msn |
NNNCCACATAGGGTTTAGCT |
NGG |
2 |
0.6125 |
Tier II |
| 2 |
NC_000067.6 |
1 |
59288689 |
+ |
Cdk15 |
NNNACACACAGGGTTTAGTG |
NGG |
2 |
0.3887 |
Tier II |
| 3 |
NC_000080.6 |
14 |
121123162 |
+ |
Farp1 |
NNNCCACACAGGGTTTAGTT |
NGG |
2 |
0.3231 |
Tier II |
| 4 |
NC_000077.6 |
11 |
84010247 |
- |
Synrg |
NNNCCACACAGGGTTTAAAG |
NGG |
2 |
0.2967 |
Tier II |
| 5 |
NC_000071.6 |
5 |
66394526 |
- |
Apbb2 |
NNNCCACACAGGGTTTTGAG |
NGG |
2 |
0.0571 |
Tier II |
Other clones with same target sequence:
(none)
