Construct: sgRNA BRDN0001147742

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TATACCGAAGAAGTCAGACA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NTPCR (84284)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76877

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 232955648 + NTPCR NNNACCGAAGAAGTCAGACA NGG 0 1.0 Tier I
2 NC_000006.12 6 130175898 - SAMD3 NNNACCGAAGAGGCCAGACA NGG 2 0.2063 Tier I
3 NC_000009.12 9 96818523 - ZNF782 NNNACCGAAGAACTCACACA NGG 2 0.0991 Tier I
4 NC_000014.9 14 76480919 - ESRRB NNNACACAAGAAGTCAGACA NGG 2 0.6384 Tier II
5 NC_000008.11 8 120598513 + SNTB1 NNNAGTGAAGAAGTCAGACA NGG 2 0.5571 Tier II
6 NC_000015.10 15 74570364 - ARID3B NNNACCTAAAAAGTCAGACA NGG 2 0.5333 Tier II
7 NC_000007.14 7 114176652 - FOXP2 NNNACCTAAGAAATCAGACA NGG 2 0.5275 Tier II
8 NC_000012.12 12 100239973 - DEPDC4 NNNAACTAAGAAGTCAGACA NGG 2 0.3265 Tier II
9 NC_000011.10 11 20119591 - NAV2 NNNACCGAAGAGGTCAGGCA NGG 2 0.2889 Tier II
10 NC_000009.12 9 5768720 - RIC1 NNNACAGAAGAAGTCAGACC NGG 2 0.211 Tier II
11 NC_000004.12 4 13873048 - LINC01182 NNNACTAAAGAAGTCAGACA NGG 2 0.9286 Tier III
12 NC_000004.12 4 13873048 - LOC107986182 NNNACTAAAGAAGTCAGACA NGG 2 0.9286 Tier III
13 NC_000009.12 9 14931344 + LOC124902122 NNNACAGTAGAAGTCAGACA NGG 2 0.398 Tier III
14 NC_000011.10 11 20119591 - LOC107984418 NNNACCGAAGAGGTCAGGCA NGG 2 0.2889 Tier III
15 NC_000014.9 14 49084416 - LOC105378178 NNNACAGAAGAAGTCAGAGA NGG 2 0.1161 Tier III
16 NC_000008.11 8 23745536 - LOC107986930 NNNACCCAAGAAGGCAGACA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000072.6 6 101151450 - Pdzrn3 NNNGCCGGAGAAGTCAGACA NGG 2 0.4583 Tier I
2 NC_000076.6 10 26251860 + Samd3 NNNACCGAAGAGGCCAGACA NGG 2 0.2063 Tier I
3 NC_000068.7 2 156038100 + Fer1l4 NNNACTGAGGAAGTCAGACA NGG 2 0.619 Tier II
4 NC_000083.6 17 45534026 - Tcte1 NNNAACGAAGAAATCAGACA NGG 2 0.5275 Tier II
5 NC_000083.6 17 65936051 + Twsg1 NNNTCCGAAGAAGACAGACA NGG 2 0.3939 Tier II
6 NC_000079.6 13 92477681 + Fam151b NNNACTGAAGAAGTCAGGCA NGG 2 0.3714 Tier II
7 NC_000073.6 7 49608129 - Nav2 NNNACCGAAGAGGTCAGGCA NGG 2 0.2889 Tier II
8 NC_000077.6 11 93249303 + Car10 NNNAAGGAAGAAGTCAGACA NGG 2 0.2857 Tier II
9 NC_000079.6 13 12305172 + Actn2 NNNCCCCAAGAAGTCAGACA NGG 2 0.2426 Tier II
10 NC_000067.6 1 17023558 + Jph1 NNNACCCAAGATGTCAGACA NGG 2 0.2292 Tier II
11 NC_000082.6 16 9589247 + Grin2a NNNACCCAAGATGTCAGACA NGG 2 0.2292 Tier II
12 NC_000067.6 1 180906978 - Pycr2 NNNACGGAAGAACTCAGACA NGG 2 0.2105 Tier II
13 NC_000086.7 X 83334750 - Dmd NNNACCGCAGAAGTCAGATA NGG 2 0.1978 Tier II
14 NC_000085.6 19 53613962 + Smc3 NNNACCTAAGAAGTCATACA NGG 2 0.1429 Tier II
15 NC_000071.6 5 137762364 - Tsc22d4 NNNACCTAAGAAGTAAGACA NGG 2 0.127 Tier II
16 NC_000072.6 6 108541492 + Itpr1 NNNACGGAAGAAGTCAGACC NGG 2 0.1136 Tier II
17 NC_000071.6 5 91393405 + Btc NNNACCAAAGAAGTCAGACA NCG 2 0.1071 Tier II
18 NC_000072.6 6 28710145 - Snd1 NNNACCCAAGAAGTCAGACA NGT 2 0.0111 Tier II
19 NC_000070.6 4 142358081 + Gm46875 NNNACAGAAGAATTCAGACA NGG 2 0.2786 Tier III
20 NC_000080.6 14 13234848 + Gm5087 NNNACCTAAGAAGTTAGACA NGG 2 0.0381 Tier III
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Other clones with same target sequence:

(none)