Construct: sgRNA BRDN0001147750

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGGATGCGTACATCAACCAC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PKN2 (5586)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77926

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 88805634 - PKN2 NNNATGCGTACATCAACCAC NGG 0 1.0 Tier I
2 NC_000016.10 16 20434280 - ACSM5 NNNATGCATACATCAACCAC NGA 2 0.0694 Tier II
3 NC_000008.11 8 23372600 + LOXL2 NNNATGCGTACATTAACCAG NGG 2 0.0431 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000071.6 5 20555316 + Magi2 NNNATGTGTTCATCAACCAC NGG 2 0.7169 Tier II
2 NC_000074.6 8 20906847 + Gm6040 NNNATGCATACATCAGCCAC NGG 2 0.1923 Tier II
3 NC_000068.7 2 72946530 - Sp3 NNNATGCATACATCAACCAC NTG 2 0.039 Tier II
4 NC_000067.6 1 106289530 - Phlpp1 NNNATGCGTACTTCATCCAC NGG 2 0.0 Tier II
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Other clones with same target sequence:

(none)