Construct: sgRNA BRDN0001147751
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAAGGAGAAGCTTATTCGTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BUB1 (699)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76772
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
110667596 |
- |
BUB1 |
NNNGGAGAAGCTTATTCGTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
7596342 |
- |
ADCY2 |
NNNGGAGATACTTATTCGTG |
NGG |
2 |
0.56 |
Tier II |
| 3 |
NC_000013.11 |
13 |
29111954 |
+ |
MTUS2 |
NNNAGAGAAGCTTATTTGTG |
NGG |
2 |
0.42 |
Tier II |
| 4 |
NC_000003.12 |
3 |
121008191 |
- |
STXBP5L |
NNNGGAGAAGCTTATTATTG |
NGG |
2 |
0.3111 |
Tier II |
| 5 |
NC_000015.10 |
15 |
66243203 |
+ |
MEGF11 |
NNNGGAGAAGCTGATTCCTG |
NGG |
2 |
0.1242 |
Tier II |
| 6 |
NC_000002.12 |
2 |
238142564 |
+ |
KLHL30 |
NNNGGAGAAGCTTATGCGTG |
NCG |
2 |
0.0371 |
Tier II |
| 7 |
NC_000012.12 |
12 |
117015560 |
+ |
FBXW8 |
NNNGGAGAAGCTTATCCGTG |
NTG |
2 |
0.026 |
Tier II |
| 8 |
NC_000017.11 |
17 |
76176053 |
+ |
RNF157 |
NNNGGAGAAGCTTGTTCGTG |
NGC |
2 |
0.0104 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
127821750 |
- |
Bub1 |
NNNGGAAAAGCTTATTCGTG |
NAG |
2 |
0.2593 |
Tier I |
| 2 |
NC_000074.6 |
8 |
78347067 |
+ |
Ttc29 |
NNNGGAGATGCTTACTCGTG |
NGG |
2 |
0.1636 |
Tier II |
| 3 |
NC_000072.6 |
6 |
145178660 |
- |
Casc1 |
NNNGGAGAAGCTGATTCCTG |
NGG |
2 |
0.1242 |
Tier II |
| 4 |
NC_000071.6 |
5 |
19505679 |
- |
Magi2 |
NNNGGAGAAGCTGATGCGTG |
NGG |
2 |
0.0903 |
Tier II |
| 5 |
NC_000073.6 |
7 |
127115301 |
- |
Qprt |
NNNGGTGAAGCTTATTGGTG |
NGG |
2 |
0.042 |
Tier II |
| 6 |
NC_000085.6 |
19 |
39254997 |
- |
Cyp2c53-ps |
NNNGGAGAAGTTTATTCCTG |
NGG |
2 |
0.1465 |
Tier III |
| 7 |
NC_000071.6 |
5 |
19505679 |
- |
Gm21009 |
NNNGGAGAAGCTGATGCGTG |
NGG |
2 |
0.0903 |
Tier III |
Other clones with same target sequence:
(none)
