Construct: sgRNA BRDN0001147752
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTAGGTGAAGAAGATCGTTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PI4KA (5297)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76819
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
20811020 |
- |
PI4KA |
NNNGGTGAAGAAGATCGTTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000014.9 |
14 |
50628137 |
+ |
ATL1 |
NNNGGTGAAGAAGATGGGTG |
NGG |
2 |
0.0513 |
Tier I |
| 3 |
NC_000005.10 |
5 |
137628369 |
- |
KLHL3 |
NNNGGTGAGGAAGAGCGTTG |
NGG |
2 |
0.0333 |
Tier I |
| 4 |
NC_000001.11 |
1 |
52509289 |
- |
TUT4 |
NNNGGTGAATAAGATAGTTG |
NGG |
2 |
0.8125 |
Tier II |
| 5 |
NC_000022.11 |
22 |
32750113 |
+ |
SYN3 |
NNNGGTGAATAAGATCGATG |
NGG |
2 |
0.5417 |
Tier II |
| 6 |
NC_000004.12 |
4 |
94660620 |
- |
PDLIM5 |
NNNGGTGAAGAAGACAGTTG |
NGG |
2 |
0.2727 |
Tier II |
| 7 |
NC_000017.11 |
17 |
3289805 |
- |
OR3A2 |
NNNGGTCAAGAAGACCGTTG |
NGG |
2 |
0.1875 |
Tier II |
| 8 |
NC_000008.11 |
8 |
118343492 |
+ |
SAMD12 |
NNNGGTGATGAAGATCTTTG |
NGG |
2 |
0.15 |
Tier II |
| 9 |
NC_000003.12 |
3 |
171624947 |
+ |
PLD1 |
NNNGGTGAAAAAGATGGTTG |
NGG |
2 |
0.1436 |
Tier II |
| 10 |
NC_000011.10 |
11 |
70702581 |
- |
SHANK2 |
NNNGGTGAAGAAGATGATTG |
NGG |
2 |
0.1436 |
Tier II |
| 11 |
NC_000019.10 |
19 |
8447530 |
+ |
HNRNPM |
NNNGGAGAAGAAGATGGTTG |
NGG |
2 |
0.1333 |
Tier II |
| 12 |
NC_000005.10 |
5 |
113182091 |
- |
MCC |
NNNGGTGAAGAAGAGAGTTG |
NGG |
2 |
0.05 |
Tier II |
| 13 |
NC_000011.10 |
11 |
70702047 |
- |
SHANK2 |
NNNGGTGAAGAAGATGGTGG |
NGG |
2 |
0.0385 |
Tier II |
| 14 |
NC_000002.12 |
2 |
51970078 |
- |
NRXN1-DT |
NNNGGTGAAGATGTTCGTTG |
NGG |
2 |
0.1778 |
Tier III |
| 15 |
NC_000008.11 |
8 |
118343492 |
+ |
LOC105375724 |
NNNGGTGATGAAGATCTTTG |
NGG |
2 |
0.15 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000082.6 |
16 |
17364000 |
- |
Pi4ka |
NNNGGTGAAGAAGATTGTTG |
NGG |
1 |
0.3077 |
Tier I |
| 2 |
NC_000069.6 |
3 |
130626526 |
+ |
Etnppl |
NNNGGTGAAGAAGATCATTG |
NAG |
2 |
0.242 |
Tier I |
| 3 |
NC_000086.7 |
X |
73542266 |
+ |
Atp2b3 |
NNNGGTGAAGAAGATCATTG |
NGC |
2 |
0.0207 |
Tier I |
| 4 |
NC_000080.6 |
14 |
59967332 |
+ |
Atp8a2 |
NNNGGTGAAGAAAATCCTTG |
NGG |
2 |
0.2172 |
Tier II |
| 5 |
NC_000071.6 |
5 |
34183692 |
- |
Mxd4 |
NNNGGTGAAGGAGATCCTTG |
NGG |
2 |
0.1529 |
Tier II |
| 6 |
NC_000070.6 |
4 |
34636083 |
+ |
Rars2 |
NNNGGGGAAGAAGATCCTTG |
NGG |
2 |
0.1345 |
Tier II |
| 7 |
NC_000069.6 |
3 |
154949528 |
+ |
Tnni3k |
NNNGGTGGAGAAGATGGTTG |
NGG |
2 |
0.1128 |
Tier II |
| 8 |
NC_000072.6 |
6 |
34608156 |
- |
Cald1 |
NNNGGTGAAGACGATCGTGG |
NGG |
2 |
0.0658 |
Tier II |
| 9 |
NC_000077.6 |
11 |
113317825 |
+ |
Slc39a11 |
NNNGGTGAAGAAGCTCTTTG |
NGG |
2 |
0.0536 |
Tier II |
Other clones with same target sequence:
(none)
