Construct: sgRNA BRDN0001147753
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCCGAGGATCAGTATGCAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- DSTYK (25778)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76296
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
205187734 |
+ |
DSTYK |
NNNCGAGGATCAGTATGCAA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
29520902 |
+ |
IL1RAPL1 |
NNNAAAGGATCAGTATGCAA |
NGG |
2 |
0.7298 |
Tier II |
| 3 |
NC_000014.9 |
14 |
74107012 |
- |
LIN52 |
NNNCGAGGGTCAGAATGCAA |
NGG |
2 |
0.4127 |
Tier II |
| 4 |
NC_000020.11 |
20 |
24953142 |
- |
CST7 |
NNNCGAGGACCAGTGTGCAA |
NGG |
2 |
0.3467 |
Tier II |
| 5 |
NC_000001.11 |
1 |
56259683 |
+ |
LOC124904186 |
NNNCGAGGGTCAGTATGCAG |
NGG |
2 |
0.5098 |
Tier III |
| 6 |
NC_000003.12 |
3 |
28744535 |
+ |
LINC00693 |
NNNTGAGCATCAGTATGCAA |
NGG |
2 |
0.4923 |
Tier III |
| 7 |
NC_000014.9 |
14 |
74107012 |
- |
LOC105370563 |
NNNCGAGGGTCAGAATGCAA |
NGG |
2 |
0.4127 |
Tier III |
| 8 |
NC_000011.10 |
11 |
34678597 |
- |
NDUFB8P3 |
NNNCGAGGATCAGAATGCAA |
NAG |
2 |
0.1605 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
56300548 |
+ |
Habp2 |
NNNCGAGGATCAGTAAGCAA |
NGG |
1 |
0.9091 |
Tier II |
| 2 |
NC_000071.6 |
5 |
124302411 |
+ |
Mphosph9 |
NNNTGGGGATCAGTATGCAA |
NGG |
2 |
0.5714 |
Tier II |
| 3 |
NC_000075.6 |
9 |
48464189 |
- |
Gm39335 |
NNNCTAGGGTCAGTATGCAA |
NGG |
2 |
0.2 |
Tier III |
Other clones with same target sequence:
(none)
