Construct: sgRNA BRDN0001147757
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGGCTCAACCTACAAACGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP5K1A (8394)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77963
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
151234356 |
+ |
PIP5K1A |
NNNGCTCAACCTACAAACGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
43682795 |
+ |
MRPS18A |
NNNAATCAACCTACAAACGG |
NGG |
2 |
0.5143 |
Tier II |
| 3 |
NC_000014.9 |
14 |
78663456 |
+ |
NRXN3 |
NNNGCTCAACCTGCTAACGG |
NGG |
2 |
0.1304 |
Tier II |
| 4 |
NC_000019.10 |
19 |
12701046 |
- |
TNPO2 |
NNNGCTCATCCTACAAACGG |
NGA |
2 |
0.0417 |
Tier II |
| 5 |
NC_000006.12 |
6 |
7987296 |
+ |
PIP5K1P1 |
NNNGCTCAACCTACAAACGC |
NGG |
1 |
0.4286 |
Tier III |
| 6 |
NC_000006.12 |
6 |
7987296 |
+ |
BLOC1S5-TXNDC5 |
NNNGCTCAACCTACAAACGC |
NGG |
1 |
0.4286 |
Tier III |
| 7 |
NC_000014.9 |
14 |
19147073 |
- |
NBEAP5 |
NNNCCTCAACCTACAAACAG |
NGG |
2 |
0.3782 |
Tier III |
| 8 |
NC_000014.9 |
14 |
19252925 |
+ |
NBEAP6 |
NNNCCTCAACCTACAAACAG |
NGG |
2 |
0.3782 |
Tier III |
| 9 |
NC_000022.11 |
22 |
15869435 |
- |
NBEAP3 |
NNNCCTCAACCTACAAACAG |
NGG |
2 |
0.3782 |
Tier III |
| 10 |
NC_000010.11 |
10 |
93960758 |
- |
PIPSL |
NNNGCTCAACCTACAGGCGG |
NGG |
2 |
0.0339 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
32536875 |
- |
Fam102a |
NNNGCTCAACCTGCAAAAGG |
NGG |
2 |
0.3512 |
Tier II |
| 2 |
NC_000077.6 |
11 |
121670501 |
+ |
B3gntl1 |
NNNGCTCCACCTACAAACTG |
NGG |
2 |
0.2857 |
Tier II |
| 3 |
NC_000067.6 |
1 |
55917062 |
- |
Gm32949 |
NNNGCTCACCCTACACACGG |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
