Construct: sgRNA BRDN0001147767
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAACATAATAATCCATAACC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- CDC42BPA (8476)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75748
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
227193927 |
+ |
CDC42BPA |
NNNCATAATAATCCATAACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000023.11 |
X |
14640793 |
- |
GLRA2 |
NNNCATAATAATCTAAAACC |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000002.12 |
2 |
213860209 |
+ |
SPAG16 |
NNNCATAATAATTCAAAACC |
NGG |
2 |
0.6364 |
Tier II |
| 4 |
NC_000001.11 |
1 |
108156239 |
+ |
SLC25A24 |
NNNCGTAATAAACCATAACC |
NGG |
2 |
0.576 |
Tier II |
| 5 |
NC_000005.10 |
5 |
142153516 |
- |
NDFIP1 |
NNNCATAATAATCCATAAAC |
NGG |
1 |
0.4286 |
Tier II |
| 6 |
NC_000001.11 |
1 |
10310174 |
+ |
KIF1B |
NNNCATATAAATCCATAACC |
NGG |
2 |
0.398 |
Tier II |
| 7 |
NC_000012.12 |
12 |
76452693 |
+ |
OSBPL8 |
NNNCAATATAATCCATAACC |
NGG |
2 |
0.3792 |
Tier II |
| 8 |
NC_000008.11 |
8 |
115547945 |
+ |
TRPS1 |
NNNCATAATAATCCTAAACC |
NGG |
2 |
0.1818 |
Tier II |
| 9 |
NC_000010.11 |
10 |
72005397 |
+ |
CHST3 |
NNNCATAAAAATCCATTACC |
NGG |
2 |
0.1238 |
Tier II |
| 10 |
NC_000002.12 |
2 |
141667976 |
+ |
LRP1B |
NNNCATAATAATACATAACC |
NAG |
2 |
0.0997 |
Tier II |
| 11 |
NC_000007.14 |
7 |
134996954 |
- |
AGBL3 |
NNNCATAATAAACCATAACC |
NTG |
2 |
0.0312 |
Tier II |
| 12 |
NC_000002.12 |
2 |
236537519 |
+ |
ACKR3 |
NNNCATAATAATCCATGACC |
NTG |
2 |
0.0069 |
Tier II |
| 13 |
NC_000002.12 |
2 |
188215716 |
- |
LINC01090 |
NNNCATAATAAACCACAACC |
NGG |
2 |
0.5333 |
Tier III |
| 14 |
NC_000018.10 |
18 |
39662346 |
+ |
MIR924HG |
NNNCATAATAATCCATAAAT |
NGG |
2 |
0.1286 |
Tier III |
| 15 |
NC_000018.10 |
18 |
39662346 |
+ |
LOC101927857 |
NNNCATAATAATCCATAAAT |
NGG |
2 |
0.1286 |
Tier III |
| 16 |
NC_000002.12 |
2 |
141667976 |
+ |
LOC107985779 |
NNNCATAATAATACATAACC |
NAG |
2 |
0.0997 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
180040060 |
- |
Cdc42bpa |
NNNCATAATAATCCATAACC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000079.6 |
13 |
62996935 |
- |
Aopep |
NNNCACAACAATCCATAACC |
NGG |
2 |
0.8392 |
Tier II |
| 3 |
NC_000072.6 |
6 |
27931283 |
- |
Grm8 |
NNNCATGGTAATCCATAACC |
NGG |
2 |
0.5176 |
Tier II |
| 4 |
NC_000071.6 |
5 |
127437659 |
- |
Tmem132c |
NNNCATCATAAACCATAACC |
NGG |
2 |
0.35 |
Tier II |
| 5 |
NC_000077.6 |
11 |
35862671 |
+ |
Wwc1 |
NNNCATAATGATCCATAAAC |
NGG |
2 |
0.2381 |
Tier II |
| 6 |
NC_000071.6 |
5 |
52461972 |
- |
Ccdc149 |
NNNAATAATAATCCATAAGC |
NGG |
2 |
0.1053 |
Tier II |
| 7 |
NC_000072.6 |
6 |
135382327 |
- |
Emp1 |
NNNCTTAATAATCCATAACC |
NGA |
2 |
0.0253 |
Tier II |
| 8 |
NC_000067.6 |
1 |
34058556 |
+ |
Dst |
NNNCATAATAATCAATAACC |
NTG |
2 |
0.0136 |
Tier II |
| 9 |
NC_000068.7 |
2 |
102135483 |
+ |
Ldlrad3 |
NNNCATAATAATCCATGACC |
NTG |
2 |
0.0069 |
Tier II |
| 10 |
NC_000072.6 |
6 |
25164457 |
- |
Gm30658 |
NNNCAAAATAATTCATAACC |
NGG |
2 |
0.6067 |
Tier III |
Other clones with same target sequence:
(none)
