Construct: sgRNA BRDN0001147768

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
AAAAATGCGCAAATTCAGCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
PIK3CB (5291)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76194

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 138742713 - PIK3CB NNNAATGCGCAAATTCAGCG NGG 0 1.0 Tier I
2 NC_000006.12 6 101682923 + GRIK2 NNNAAAGCGCAAATTCAGCC NGG 2 0.3714 Tier II
3 NC_000008.11 8 130131840 + ASAP1 NNNAATGCGCAACTTCAGGG NGG 2 0.0263 Tier II
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000075.6 9 99094595 - Pik3cb NNNAATGCGCAAATTCAGTG NGG 1 0.4615 Tier I
2 NC_000077.6 11 33768620 - Kcnip1 NNNAACGCTCAAATTCAGCG NGG 2 0.4848 Tier II
3 NC_000068.7 2 155249407 + Dynlrb1 NNNAATGCTCAAAATCAGCG NGG 2 0.3302 Tier II
Download CSV

Other clones with same target sequence:

(none)