Construct: sgRNA BRDN0001147770
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TTAGGCATGCCATGTCGAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MPP2 (4355)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77809
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
43881337 |
- |
MPP2 |
NNNGGCATGCCATGTCGAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
63837533 |
+ |
SMARCD2 |
NNNGGCATGCCAGGTCGAAG |
NGG |
1 |
0.2609 |
Tier I |
| 3 |
NC_000007.14 |
7 |
93215599 |
- |
HEPACAM2 |
NNNGACATGCCATGTGGAAG |
NGG |
2 |
0.1333 |
Tier I |
| 4 |
NC_000001.11 |
1 |
41829194 |
- |
HIVEP3 |
NNNAGCATGCCATATCGAAG |
NGG |
2 |
0.675 |
Tier II |
| 5 |
NC_000015.10 |
15 |
86586744 |
+ |
AGBL1 |
NNNGGCAGGCCATCTCGAAG |
NGG |
2 |
0.3143 |
Tier II |
| 6 |
NC_000017.11 |
17 |
20059987 |
- |
SPECC1 |
NNNGGCATGCCATGTGGAAG |
NGG |
1 |
0.1538 |
Tier II |
| 7 |
NC_000006.12 |
6 |
35427667 |
+ |
PPARD |
NNNGGCATGCCATGTCTGAG |
NGG |
2 |
0.1 |
Tier II |
| 8 |
NC_000022.11 |
22 |
36321376 |
+ |
MYH9 |
NNNGGCATGGCATGTCCAAG |
NGG |
2 |
0.0915 |
Tier II |
| 9 |
NC_000016.10 |
16 |
86088916 |
+ |
LOC124903742 |
NNNGGCATGCCATGGCTAAG |
NGG |
2 |
0.0125 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
106267426 |
+ |
Smarcd2 |
NNNGGCATGCCAGGTCGGAG |
NGG |
2 |
0.1043 |
Tier I |
| 2 |
NC_000071.6 |
5 |
90961174 |
+ |
Mthfd2l |
NNNGGCATGCTATGTAGAAG |
NGG |
2 |
0.3077 |
Tier II |
| 3 |
NC_000083.6 |
17 |
85236440 |
+ |
Camkmt |
NNNGACATGCCATGCCGAAG |
NGG |
2 |
0.2364 |
Tier II |
| 4 |
NC_000082.6 |
16 |
97582717 |
- |
Tmprss2 |
NNNGGCATTCCATGTTGAAG |
NGG |
2 |
0.1641 |
Tier II |
| 5 |
NC_000071.6 |
5 |
101721774 |
+ |
9430085M18Rik |
NNNGGCGTGCCATGTGGAAG |
NGG |
2 |
0.1086 |
Tier III |
Other clones with same target sequence:
(none)
