Construct: sgRNA BRDN0001147772

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATAGCACTTTAAAAAAGGCA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
BMPR1A (657)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77960

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000010.11 10 86890164 - BMPR1A NNNGCACTTTAAAAAAGGCA NGG 0 1.0 Tier I
2 NC_000017.11 17 64213391 + TEX2 NNNGCACTTTAAAAAAGGAA NGA 2 0.0298 Tier I
3 NC_000010.11 10 12232146 - CDC123 NNNGCATTTAAAAAAAGGCA NGG 2 0.6964 Tier II
4 NC_000002.12 2 211995441 - ERBB4 NNNGTATTTTAAAAAAGGCA NGG 2 0.517 Tier II
5 NC_000006.12 6 129158952 + LAMA2 NNNGCACTTGAAAAAAGGCA NGG 1 0.5 Tier II
6 NC_000003.12 3 169631146 - MECOM NNNGCAATTTAAAAGAGGCA NGG 2 0.4875 Tier II
7 NC_000012.12 12 108326829 - CMKLR1 NNNGCACTTAAAATAAGGCA NGG 2 0.4571 Tier II
8 NC_000004.12 4 122245400 - BLTP1 NNNGCACTTTAGAAAAGGCT NGG 2 0.4333 Tier II
9 NC_000006.12 6 16259006 - GMPR NNNGCACATGAAAAAAGGCA NGG 2 0.4 Tier II
10 NC_000002.12 2 141026397 + LRP1B NNNGAACTTTAAAAAAGACA NGG 2 0.3956 Tier II
11 NC_000004.12 4 142296089 + INPP4B NNNGCATTTTAAAAAAGGAA NGG 2 0.3482 Tier II
12 NC_000016.10 16 68776623 - CDH1 NNNGCACTTTGAAAAAGCCA NGG 2 0.3095 Tier II
13 NC_000010.11 10 96005389 + CC2D2B NNNGCTCTTTAAAAAAGGAA NGG 2 0.3061 Tier II
14 NC_000015.10 15 59179712 + MYO1E NNNGCACTGTAAAAAAGCCA NGG 2 0.2948 Tier II
15 NC_000010.11 10 95771521 - ENTPD1 NNNGCTCTTTCAAAAAGGCA NGG 2 0.2857 Tier II
16 NC_000001.11 1 153202920 + SPRR2G NNNGGCCTTTAAAAAAGGCA NGG 2 0.2727 Tier II
17 NC_000001.11 1 174432869 + RABGAP1L NNNGTACTTTAAAAAAGGAA NGG 2 0.2727 Tier II
18 NC_000003.12 3 169165562 - MECOM NNNGCACTTCAAAAAAGCCA NGG 2 0.254 Tier II
19 NC_000017.11 17 21205530 - TMEM11 NNNACACTTTAAAAAAGGCA NAG 2 0.2333 Tier II
20 NC_000007.14 7 116884157 - CAPZA2 NNNGCAATTTTAAAAAGGCA NGG 2 0.2308 Tier II
21 NC_000020.11 20 20329328 + CFAP61 NNNCCACTTTAAAAAAGGAA NGG 2 0.2269 Tier II
22 NC_000010.11 10 95980102 - CC2D2B NNNGTACTTTATAAAAGGCA NGG 2 0.2121 Tier II
23 NC_000013.11 13 77591534 + SCEL NNNGCACATTAAAAAATGCA NGG 2 0.2 Tier II
24 NC_000006.12 6 150156255 + PPP1R14C NNNTCACTTTAAATAAGGCA NGG 2 0.1939 Tier II
25 NC_000009.12 9 132782327 - AK8 NNNTCACTTTAAAGAAGGCA NGG 2 0.1697 Tier II
26 NC_000007.14 7 147510738 + CNTNAP2 NNNGCACTTTACAAAAGGCT NGG 2 0.1579 Tier II
27 NC_000017.11 17 63177624 + TANC2 NNNGCACTTTAAAAAACTCA NGG 2 0.1569 Tier II
28 NC_000001.11 1 237679965 + RYR2 NNNGCACTTTAAGAAAGGCC NGG 2 0.1482 Tier II
29 NC_000003.12 3 74297583 + CNTN3 NNNGCACTTTACATAAGGCA NGG 2 0.1404 Tier II
30 NC_000016.10 16 88201319 + ZNF469 NNNGCACTTTAAAAAGGTCA NGG 2 0.1282 Tier II
31 NC_000004.12 4 8404566 - ACOX3 NNNGCCCTTTAAAACAGGCA NGG 2 0.124 Tier II
32 NC_000008.11 8 143764064 - IQANK1 NNNGAACTTTAAACAAGGCA NGG 2 0.1224 Tier II
33 NC_000009.12 9 3900799 - GLIS3 NNNGGACTTTAAAAAAGGGA NGG 2 0.075 Tier II
34 NC_000011.10 11 32723663 + CCDC73 NNNACACTTTAAAAAAGGCA NGA 2 0.0625 Tier II
35 NC_000001.11 1 67381128 - IL12RB2 NNNTCACTTTAAAAAAGGCA NGA 2 0.0253 Tier II
36 NC_000007.14 7 48442988 + ABCA13 NNNGGACTTTAAAAAAGGCA NTG 2 0.0234 Tier II
37 NC_000004.12 4 157110841 - GLRB NNNCCACTTTAAAAAAGGCA NTG 2 0.0206 Tier II
38 NC_000002.12 2 230752119 - CAB39 NNNTCACTTTAAAAAAGGCA NTG 2 0.0142 Tier II
39 NC_000010.11 10 54762765 - PCDH15 NNNGCACTTTAAAAAAGGCT NGC 2 0.0133 Tier II
40 NC_000003.12 3 171613395 + PLD1 NNNGCACTTGAAAAAAGGCA NGC 2 0.0111 Tier II
41 NC_000012.12 12 29353131 + ERGIC2 NNNGCACTTTAAAAAAGGCA NTA 2 0.0 Tier II
42 NC_000013.11 13 112391666 + SPACA7 NNNGCACTTTAAAAACCGCA NGG 2 0.0 Tier II
43 NC_000005.10 5 127540679 + PRRC1 NNNGCACTTTAAAAATGTCA NGG 2 0.0 Tier II
44 NC_000006.12 6 129158952 + BMPR1AP1 NNNGCACTTGAAAAAAGGCA NGG 1 0.5 Tier III
45 NC_000010.11 10 96005389 + ENTPD1-AS1 NNNGCTCTTTAAAAAAGGAA NGG 2 0.3061 Tier III
46 NC_000010.11 10 95771521 - ENTPD1-AS1 NNNGCTCTTTCAAAAAGGCA NGG 2 0.2857 Tier III
47 NC_000013.11 13 48120945 + LOC105370198 NNNGCAATTTTAAAAAGGCA NGG 2 0.2308 Tier III
48 NC_000010.11 10 95980102 - ENTPD1-AS1 NNNGTACTTTATAAAAGGCA NGG 2 0.2121 Tier III
49 NC_000001.11 1 53337442 - LOC105378731 NNNGCACTTTTAGAAAGGCA NGG 2 0.2007 Tier III
50 NC_000017.11 17 79709539 + LINC02078 NNNGCACTTCAAACAAGGCA NGG 2 0.1143 Tier III
51 NC_000019.10 19 19946192 - BNIP3P12 NNNTCACTTTAAAAAAGGCC NGG 2 0.0826 Tier III
52 NC_000009.12 9 3900799 - GLIS3-AS1 NNNGGACTTTAAAAAAGGGA NGG 2 0.075 Tier III
53 NC_000009.12 9 80027268 - LINC01507 NNNGCACTTGAAAAAAGGGA NGG 2 0.0625 Tier III
54 NC_000003.12 3 145572459 - LOC105374143 NNNGCACTTTAAAAAATGCC NGG 2 0.0568 Tier III
55 NC_000005.10 5 17152296 + BASP1-AS1 NNNGGACTTTAAAAAAGGCA NTG 2 0.0234 Tier III
56 NC_000023.11 X 145845451 + LOC107985703 NNNGCACTTTAAAAAAAGCA NGT 2 0.0151 Tier III
57 NC_000010.11 10 79909921 - BMS1P21 NNNGCACTTTAAAAAAGGCT NGT 2 0.0097 Tier III
58 NC_000007.14 7 13644948 + LOC107986770 NNNCCACTTTAAAAAAGGCA NGT 2 0.0085 Tier III
59 NC_000013.11 13 112391666 + LOC105370372 NNNGCACTTTAAAAACCGCA NGG 2 0.0 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000080.6 14 34443880 + Bmpr1a NNNGCACTTTAAGAAAGGCA NGG 1 0.6522 Tier I
2 NC_000082.6 16 52047413 + Cblb NNNGCTCTTTAAAGAAGGCA NGG 2 0.3333 Tier I
3 NC_000077.6 11 106567779 + Tex2 NNNGCACTTTAAAAAAGGAA NGA 2 0.0298 Tier I
4 NC_000072.6 6 22206069 + Cped1 NNNGCAATTTAAAAAAAGCA NGG 2 0.7 Tier II
5 NC_000077.6 11 116930641 + Mgat5b NNNGCACTTTAAAAAAAACA NGG 2 0.6462 Tier II
6 NC_000075.6 9 112213057 - Arpp21 NNNGCAATTAAAAAAAGGCA NGG 2 0.6429 Tier II
7 NC_000068.7 2 112238293 - Gm52523 NNNGCAATTTAAGAAAGGCA NGG 2 0.4891 Tier II
8 NC_000067.6 1 160769458 - Rabgap1l NNNGCACCTTAAAGAAGGCA NGG 2 0.4667 Tier II
9 NC_000072.6 6 127136414 - Ccnd2 NNNGCACTTTAAGAGAGGCA NGG 2 0.4239 Tier II
10 NC_000079.6 13 81730948 + Mblac2 NNNGCATTTTAAAAAAGGAA NGG 2 0.3482 Tier II
11 NC_000067.6 1 163931307 - Scyl3 NNNGCACCTTTAAAAAGGCA NGG 2 0.3077 Tier II
12 NC_000084.6 18 21851449 - Ccdc178 NNNGCACTTTAAGGAAGGCA NGG 2 0.3043 Tier II
13 NC_000079.6 13 94529718 - Ap3b1 NNNGCACTGTCAAAAAGGCA NGG 2 0.2476 Tier II
14 NC_000086.7 X 169062656 + Arhgap6 NNNGCACTTTCAAAAAGCCA NGG 2 0.1905 Tier II
15 NC_000075.6 9 71591911 - Myzap NNNTCAGTTTAAAAAAGGCA NGG 2 0.1711 Tier II
16 NC_000077.6 11 115412451 - Mrpl58 NNNGCACTTTAAAAAAGACC NGG 2 0.1573 Tier II
17 NC_000084.6 18 84939908 + Fbxo15 NNNGCAGTTTTAAAAAGGCA NGG 2 0.1448 Tier II
18 NC_000076.6 10 32284365 - Nkain2 NNNGCACTTTACATAAGGCA NGG 2 0.1404 Tier II
19 NC_000071.6 5 117551144 + Ksr2 NNNGCACTTTAAAATAGTCA NGG 2 0.1333 Tier II
20 NC_000083.6 17 5235292 + Arid1b NNNGCACTTTAACAAAGGCT NGG 2 0.1263 Tier II
21 NC_000079.6 13 74774458 + Cast NNNGCACTTTACAAAAGCCA NGG 2 0.1253 Tier II
22 NC_000081.6 15 94326482 + Adamts20 NNNGCACTTTAAAAAAGCCA NAG 2 0.1235 Tier II
23 NC_000070.6 4 140044702 + Igsf21 NNNGCACTTTAACAAAGGCA NAG 2 0.0546 Tier II
24 NC_000073.6 7 29048444 - Ryr1 NNNGCACTTTAAAAAAGGAA NGA 2 0.0298 Tier II
25 NC_000082.6 16 30009783 + 4632428C04Rik NNNACACTTTAAAAAAGGCG NGG 2 0.6882 Tier III
26 NC_000070.6 4 133644971 + Gm13213 NNNGCACATTAAAAAAGGAA NGG 2 0.3429 Tier III
27 NC_000077.6 11 54279440 - LOC115487722 NNNGCACTGTAATAAAGGCA NGG 2 0.1857 Tier III
28 NC_000068.7 2 78826927 + Gm39865 NNNTCAGTTTAAAAAAGGCA NGG 2 0.1711 Tier III
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Other clones with same target sequence:

(none)