Construct: sgRNA BRDN0001147773
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TCTAAACAGAGATGTAGCCT
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- IDNK (414328)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000009.12 | 9 | 83643437 | + | IDNK | NNNAAACAGAGATGTAGCCT | NGG | 0 | 1.0 | Tier I |
2 | NC_000009.12 | 9 | 32487579 | - | RIGI | NNNAAACACAGATGAAGCCT | NGG | 2 | 0.3117 | Tier I |
3 | NC_000007.14 | 7 | 146229522 | + | CNTNAP2 | NNNAAGCAGTGATGTAGCCT | NGG | 2 | 0.6303 | Tier II |
4 | NC_000014.9 | 14 | 89981679 | - | TDP1 | NNNAAACAAAGATGTAACCT | NGG | 2 | 0.6 | Tier II |
5 | NC_000012.12 | 12 | 109502670 | + | UBE3B | NNNAAATAGAGATGTAGCCA | NGG | 2 | 0.457 | Tier II |
6 | NC_000011.10 | 11 | 74026117 | + | C2CD3 | NNNAAACTGAGATGTAGCCT | NGG | 1 | 0.4286 | Tier II |
7 | NC_000005.10 | 5 | 153690131 | + | GRIA1 | NNNGAACAGAGATGAAGCCT | NGG | 2 | 0.3618 | Tier II |
8 | NC_000004.12 | 4 | 185165167 | + | CFAP97 | NNNAAAAAGATATGTAGCCT | NGG | 2 | 0.2885 | Tier II |
9 | NC_000009.12 | 9 | 29069005 | - | LINGO2 | NNNAAACTCAGATGTAGCCT | NGG | 2 | 0.2308 | Tier II |
10 | NC_000010.11 | 10 | 5164795 | - | AKR1C8 | NNNACACTGAGATGTAGCCT | NGG | 2 | 0.2143 | Tier II |
11 | NC_000008.11 | 8 | 138699650 | - | COL22A1 | NNNAACCTGAGATGTAGCCT | NGG | 2 | 0.1948 | Tier II |
12 | NC_000016.10 | 16 | 2978155 | + | PKMYT1 | NNNAGACAGAGATTTAGCCT | NGG | 2 | 0.192 | Tier II |
13 | NC_000014.9 | 14 | 72294365 | + | RGS6 | NNNCAACAGAGATGTAGACT | NGG | 2 | 0.19 | Tier II |
14 | NC_000015.10 | 15 | 57647986 | - | GCOM1 | NNNAGACAGAGCTGTAGCCT | NGG | 2 | 0.1895 | Tier II |
15 | NC_000015.10 | 15 | 57647986 | - | MYZAP | NNNAGACAGAGCTGTAGCCT | NGG | 2 | 0.1895 | Tier II |
16 | NC_000001.11 | 1 | 119379801 | - | HAO2 | NNNAAACAGAGAAGTAGCCT | NAG | 2 | 0.1795 | Tier II |
17 | NC_000001.11 | 1 | 48872331 | - | AGBL4 | NNNAAACAAAGATGCAGCCT | NGG | 2 | 0.1753 | Tier II |
18 | NC_000007.14 | 7 | 27117186 | - | HOXA3 | NNNAAACAGAGCTGTAGCCA | NGG | 2 | 0.148 | Tier II |
19 | NC_000018.10 | 18 | 46034132 | + | PSTPIP2 | NNNAAACAGAGCTGTAGCCA | NGG | 2 | 0.148 | Tier II |
20 | NC_000003.12 | 3 | 37726584 | + | ITGA9 | NNNAAACAGGGCTGTAGCCT | NGG | 2 | 0.1462 | Tier II |
21 | NC_000010.11 | 10 | 91459883 | + | HECTD2 | NNNATACAGATATGTAGCCT | NGG | 2 | 0.1399 | Tier II |
22 | NC_000004.12 | 4 | 7927091 | + | AFAP1 | NNNAATCAGAGATGTGGCCT | NGG | 2 | 0.1374 | Tier II |
23 | NC_000020.11 | 20 | 10250904 | + | SNAP25 | NNNAAACAGATTTGTAGCCT | NGG | 2 | 0.1282 | Tier II |
24 | NC_000007.14 | 7 | 111835881 | - | DOCK4 | NNNAAACAGAGATCTAGCCT | NAG | 2 | 0.1111 | Tier II |
25 | NC_000015.10 | 15 | 51470816 | + | DMXL2 | NNNAAACAGAGATGTATCAT | NGG | 2 | 0.1071 | Tier II |
26 | NC_000017.11 | 17 | 72649892 | - | SLC39A11 | NNNAAACAGAGGTGTAGGCT | NGG | 2 | 0.0963 | Tier II |
27 | NC_000002.12 | 2 | 70661485 | + | ADD2 | NNNGAACAGAGATGGAGCCT | NGG | 2 | 0.0313 | Tier II |
28 | NC_000009.12 | 9 | 137477221 | - | PNPLA7 | NNNGAACAGAGATGGAGCCT | NGG | 2 | 0.0313 | Tier II |
29 | NC_000004.12 | 4 | 142378302 | + | INPP4B | NNNAAACAGAGTTGTAGCCT | NGA | 2 | 0.0231 | Tier II |
30 | NC_000002.12 | 2 | 153388917 | + | GALNT13 | NNNAAACTGAGATGGAGCCT | NGG | 2 | 0.0214 | Tier II |
31 | NC_000002.12 | 2 | 100060251 | + | AFF3 | NNNCAACAGAGATGTAGCCT | NTG | 2 | 0.0138 | Tier II |
32 | NC_000006.12 | 6 | 106045669 | - | PRDM1 | NNNAAACAGAGATTTAGCCT | NTG | 2 | 0.0104 | Tier II |
33 | NC_000007.14 | 7 | 72326374 | - | CALN1 | NNNAAACAGAGATGTAGCCA | NGT | 2 | 0.0091 | Tier II |
34 | NC_000001.11 | 1 | 112690659 | - | MOV10 | NNNAAACAGAGATGTTGGCT | NGG | 2 | 0.0 | Tier II |
35 | NC_000003.12 | 3 | 165255864 | + | LINC01322 | NNNAAACAGAGATGTAGTCA | NGG | 2 | 0.3616 | Tier III |
36 | NC_000008.11 | 8 | 24067150 | - | LOC107986931 | NNNAAAAAGAGATGTAGCAT | NGG | 2 | 0.3214 | Tier III |
37 | NC_000023.11 | X | 27162755 | + | LOC105373150 | NNNCAGCAGAGATGTAGCCT | NGG | 2 | 0.2521 | Tier III |
38 | NC_000002.12 | 2 | 2951063 | + | LINC01250 | NNNAAACAGAGATGTGACCT | NGG | 2 | 0.1795 | Tier III |
39 | NC_000010.11 | 10 | 91459883 | + | HECTD2-AS1 | NNNATACAGATATGTAGCCT | NGG | 2 | 0.1399 | Tier III |
40 | NC_000014.9 | 14 | 59164272 | - | LOC107984642 | NNNAAACAGAGATGTAGGCT | NGG | 1 | 0.1333 | Tier III |
41 | NC_000005.10 | 5 | 78754537 | + | LOC124900191 | NNNATACAGAGTTGTAGCCT | NGG | 2 | 0.1212 | Tier III |
42 | NC_000006.12 | 6 | 4164607 | - | LOC124901246 | NNNAAACAGAGATTTGGCCT | NGG | 2 | 0.0513 | Tier III |
43 | NC_000006.12 | 6 | 131415121 | + | LOC105378005 | NNNACACAGAGATGTAGCCT | NGA | 2 | 0.0347 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000079.6 | 13 | 58163441 | + | Idnk | NNNAAACAGAGATGTGGCTT | NGG | 2 | 0.0888 | Tier I |
2 | NC_000084.6 | 18 | 37980201 | + | Arap3 | NNNAAACAGAGATGTAGCCA | NCG | 2 | 0.0603 | Tier I |
3 | NC_000068.7 | 2 | 69479903 | + | Lrp2 | NNNAAACAGAAATATAGCCT | NGG | 2 | 0.75 | Tier II |
4 | NC_000068.7 | 2 | 181508478 | + | Tpd52l2 | NNNAAGCAGTGATGTAGCCT | NGG | 2 | 0.6303 | Tier II |
5 | NC_000076.6 | 10 | 26367011 | - | L3mbtl3 | NNNAAACACAAATGTAGCCT | NGG | 2 | 0.5385 | Tier II |
6 | NC_000071.6 | 5 | 96627325 | - | Fras1 | NNNAAAGAGAGATGTAGCCT | NGG | 1 | 0.4706 | Tier II |
7 | NC_000076.6 | 10 | 122932584 | + | Ppm1h | NNNAAGCAGAGATGTAGTCT | NGG | 2 | 0.4592 | Tier II |
8 | NC_000067.6 | 1 | 192993522 | - | Syt14 | NNNAAACAGAGGTGAAGCCT | NGG | 2 | 0.4181 | Tier II |
9 | NC_000068.7 | 2 | 162399122 | + | Ptprt | NNNAAGCAGAGATGTAGCCA | NGG | 2 | 0.4018 | Tier II |
10 | NC_000067.6 | 1 | 164363438 | + | Nme7 | NNNAGACAGAGATGTAGACT | NGG | 2 | 0.3877 | Tier II |
11 | NC_000084.6 | 18 | 44686753 | - | Mcc | NNNAAATAGAGATGTAGCTT | NGG | 2 | 0.375 | Tier II |
12 | NC_000070.6 | 4 | 126298662 | + | Col8a2 | NNNCAACAGAGATGTAGCCT | NGG | 1 | 0.3529 | Tier II |
13 | NC_000081.6 | 15 | 3475263 | + | Ghr | NNNAAACAGAATTGTAGCCT | NGG | 2 | 0.3333 | Tier II |
14 | NC_000075.6 | 9 | 90183104 | + | Adamts7 | NNNAACCAGAGAAGTAGCCT | NGG | 2 | 0.3147 | Tier II |
15 | NC_000077.6 | 11 | 68287891 | - | Ntn1 | NNNAGACAGAGATGCAGCCT | NGG | 2 | 0.1964 | Tier II |
16 | NC_000071.6 | 5 | 37392714 | - | Evc2 | NNNAAGCAGAGCTGTAGCCT | NGG | 2 | 0.188 | Tier II |
17 | NC_000084.6 | 18 | 78119524 | - | Slc14a1 | NNNAGACAGAGATGTAGGCT | NGG | 2 | 0.096 | Tier II |
18 | NC_000074.6 | 8 | 23681173 | - | Zmat4 | NNNAAACAGAGCTGTATCCT | NGG | 2 | 0.0658 | Tier II |
19 | NC_000078.6 | 12 | 98752687 | - | Zc3h14 | NNNAAACAGAGAAGTAGCCT | NGA | 2 | 0.0481 | Tier II |
20 | NC_000072.6 | 6 | 55122547 | + | Crhr2 | NNNAAACAGAGATGTAGGCT | NAG | 2 | 0.0346 | Tier II |
21 | NC_000084.6 | 18 | 63904464 | + | Wdr7 | NNNAAACTGAGATGTAGCCT | NGA | 2 | 0.0298 | Tier II |
22 | NC_000082.6 | 16 | 44348718 | - | Spice1 | NNNAAACAGAGAAGTAGCCT | NTG | 2 | 0.027 | Tier II |
23 | NC_000076.6 | 10 | 24709010 | - | Enpp1 | NNNAAACACAGATGGAGCCT | NGG | 2 | 0.0269 | Tier II |
24 | NC_000080.6 | 14 | 67881814 | + | Dock5 | NNNAAACATAGATGTAGCCT | NTG | 2 | 0.0208 | Tier II |
25 | NC_000083.6 | 17 | 65407750 | - | Tmem232 | NNNAAACATAGATGTAGCCT | NTG | 2 | 0.0208 | Tier II |
26 | NC_000083.6 | 17 | 28706439 | + | Mapk14 | NNNATACAGAGATGGAGCCT | NGG | 2 | 0.0182 | Tier II |
27 | NC_000083.6 | 17 | 67884337 | + | Arhgap28 | NNNCAACAGAGATGGAGCCT | NGG | 2 | 0.0176 | Tier II |
28 | NC_000079.6 | 13 | 76065984 | + | Arsk | NNNAAAAAGAGATGTAGCCT | NGT | 2 | 0.0121 | Tier II |
29 | NC_000077.6 | 11 | 46704197 | + | Timd2 | NNNAACCAGAGATGTAGCCT | NGT | 2 | 0.0073 | Tier II |
30 | NC_000068.7 | 2 | 128705649 | + | Mertk | NNNAAACAGAGATGTTGCCT | NTG | 2 | 0.0 | Tier II |
31 | NC_000076.6 | 10 | 66149744 | - | 1700023F02Rik | NNNAAACTGAGATGTAGCCT | NGG | 1 | 0.4286 | Tier III |
32 | NC_000076.6 | 10 | 66149744 | - | 4930407I19Rik | NNNAAACTGAGATGTAGCCT | NGG | 1 | 0.4286 | Tier III |
33 | NC_000073.6 | 7 | 69800636 | + | Gm35209 | NNNAAACAGAAATTTAGCCT | NGG | 2 | 0.2667 | Tier III |
34 | NC_000071.6 | 5 | 97345242 | + | Gm33969 | NNNAATCAGAGATGCAGCCT | NGG | 2 | 0.1948 | Tier III |
35 | NC_000085.6 | 19 | 28692108 | - | D930032P07Rik | NNNAAACAGAGATGTGGTCT | NGG | 2 | 0.1236 | Tier III |
36 | NC_000073.6 | 7 | 71159495 | - | Gm36071 | NNNAAACACAGATGGAGCCT | NGG | 2 | 0.0269 | Tier III |
37 | NC_000085.6 | 19 | 10762716 | - | A430093F15Rik | NNNAAACACAGATGGAGCCT | NGG | 2 | 0.0269 | Tier III |
38 | NC_000068.7 | 2 | 128187581 | + | Gm14009 | NNNAAACAGAGATGTATCCC | NGG | 2 | 0.0227 | Tier III |
39 | NC_000079.6 | 13 | 76065984 | + | Gm41006 | NNNAAAAAGAGATGTAGCCT | NGT | 2 | 0.0121 | Tier III |
40 | NC_000068.7 | 2 | 136604902 | - | Gm32659 | NNNAAACAGAGATGTAGGCT | NGT | 2 | 0.0022 | Tier III |