Construct: sgRNA BRDN0001147773

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TCTAAACAGAGATGTAGCCT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
IDNK (414328)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75866

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000009.12 9 83643437 + IDNK NNNAAACAGAGATGTAGCCT NGG 0 1.0 Tier I
2 NC_000009.12 9 32487579 - RIGI NNNAAACACAGATGAAGCCT NGG 2 0.3117 Tier I
3 NC_000007.14 7 146229522 + CNTNAP2 NNNAAGCAGTGATGTAGCCT NGG 2 0.6303 Tier II
4 NC_000014.9 14 89981679 - TDP1 NNNAAACAAAGATGTAACCT NGG 2 0.6 Tier II
5 NC_000012.12 12 109502670 + UBE3B NNNAAATAGAGATGTAGCCA NGG 2 0.457 Tier II
6 NC_000011.10 11 74026117 + C2CD3 NNNAAACTGAGATGTAGCCT NGG 1 0.4286 Tier II
7 NC_000005.10 5 153690131 + GRIA1 NNNGAACAGAGATGAAGCCT NGG 2 0.3618 Tier II
8 NC_000004.12 4 185165167 + CFAP97 NNNAAAAAGATATGTAGCCT NGG 2 0.2885 Tier II
9 NC_000009.12 9 29069005 - LINGO2 NNNAAACTCAGATGTAGCCT NGG 2 0.2308 Tier II
10 NC_000010.11 10 5164795 - AKR1C8 NNNACACTGAGATGTAGCCT NGG 2 0.2143 Tier II
11 NC_000008.11 8 138699650 - COL22A1 NNNAACCTGAGATGTAGCCT NGG 2 0.1948 Tier II
12 NC_000016.10 16 2978155 + PKMYT1 NNNAGACAGAGATTTAGCCT NGG 2 0.192 Tier II
13 NC_000014.9 14 72294365 + RGS6 NNNCAACAGAGATGTAGACT NGG 2 0.19 Tier II
14 NC_000015.10 15 57647986 - GCOM1 NNNAGACAGAGCTGTAGCCT NGG 2 0.1895 Tier II
15 NC_000015.10 15 57647986 - MYZAP NNNAGACAGAGCTGTAGCCT NGG 2 0.1895 Tier II
16 NC_000001.11 1 119379801 - HAO2 NNNAAACAGAGAAGTAGCCT NAG 2 0.1795 Tier II
17 NC_000001.11 1 48872331 - AGBL4 NNNAAACAAAGATGCAGCCT NGG 2 0.1753 Tier II
18 NC_000007.14 7 27117186 - HOXA3 NNNAAACAGAGCTGTAGCCA NGG 2 0.148 Tier II
19 NC_000018.10 18 46034132 + PSTPIP2 NNNAAACAGAGCTGTAGCCA NGG 2 0.148 Tier II
20 NC_000003.12 3 37726584 + ITGA9 NNNAAACAGGGCTGTAGCCT NGG 2 0.1462 Tier II
21 NC_000010.11 10 91459883 + HECTD2 NNNATACAGATATGTAGCCT NGG 2 0.1399 Tier II
22 NC_000004.12 4 7927091 + AFAP1 NNNAATCAGAGATGTGGCCT NGG 2 0.1374 Tier II
23 NC_000020.11 20 10250904 + SNAP25 NNNAAACAGATTTGTAGCCT NGG 2 0.1282 Tier II
24 NC_000007.14 7 111835881 - DOCK4 NNNAAACAGAGATCTAGCCT NAG 2 0.1111 Tier II
25 NC_000015.10 15 51470816 + DMXL2 NNNAAACAGAGATGTATCAT NGG 2 0.1071 Tier II
26 NC_000017.11 17 72649892 - SLC39A11 NNNAAACAGAGGTGTAGGCT NGG 2 0.0963 Tier II
27 NC_000002.12 2 70661485 + ADD2 NNNGAACAGAGATGGAGCCT NGG 2 0.0313 Tier II
28 NC_000009.12 9 137477221 - PNPLA7 NNNGAACAGAGATGGAGCCT NGG 2 0.0313 Tier II
29 NC_000004.12 4 142378302 + INPP4B NNNAAACAGAGTTGTAGCCT NGA 2 0.0231 Tier II
30 NC_000002.12 2 153388917 + GALNT13 NNNAAACTGAGATGGAGCCT NGG 2 0.0214 Tier II
31 NC_000002.12 2 100060251 + AFF3 NNNCAACAGAGATGTAGCCT NTG 2 0.0138 Tier II
32 NC_000006.12 6 106045669 - PRDM1 NNNAAACAGAGATTTAGCCT NTG 2 0.0104 Tier II
33 NC_000007.14 7 72326374 - CALN1 NNNAAACAGAGATGTAGCCA NGT 2 0.0091 Tier II
34 NC_000001.11 1 112690659 - MOV10 NNNAAACAGAGATGTTGGCT NGG 2 0.0 Tier II
35 NC_000003.12 3 165255864 + LINC01322 NNNAAACAGAGATGTAGTCA NGG 2 0.3616 Tier III
36 NC_000008.11 8 24067150 - LOC107986931 NNNAAAAAGAGATGTAGCAT NGG 2 0.3214 Tier III
37 NC_000023.11 X 27162755 + LOC105373150 NNNCAGCAGAGATGTAGCCT NGG 2 0.2521 Tier III
38 NC_000002.12 2 2951063 + LINC01250 NNNAAACAGAGATGTGACCT NGG 2 0.1795 Tier III
39 NC_000010.11 10 91459883 + HECTD2-AS1 NNNATACAGATATGTAGCCT NGG 2 0.1399 Tier III
40 NC_000014.9 14 59164272 - LOC107984642 NNNAAACAGAGATGTAGGCT NGG 1 0.1333 Tier III
41 NC_000005.10 5 78754537 + LOC124900191 NNNATACAGAGTTGTAGCCT NGG 2 0.1212 Tier III
42 NC_000006.12 6 4164607 - LOC124901246 NNNAAACAGAGATTTGGCCT NGG 2 0.0513 Tier III
43 NC_000006.12 6 131415121 + LOC105378005 NNNACACAGAGATGTAGCCT NGA 2 0.0347 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000079.6 13 58163441 + Idnk NNNAAACAGAGATGTGGCTT NGG 2 0.0888 Tier I
2 NC_000084.6 18 37980201 + Arap3 NNNAAACAGAGATGTAGCCA NCG 2 0.0603 Tier I
3 NC_000068.7 2 69479903 + Lrp2 NNNAAACAGAAATATAGCCT NGG 2 0.75 Tier II
4 NC_000068.7 2 181508478 + Tpd52l2 NNNAAGCAGTGATGTAGCCT NGG 2 0.6303 Tier II
5 NC_000076.6 10 26367011 - L3mbtl3 NNNAAACACAAATGTAGCCT NGG 2 0.5385 Tier II
6 NC_000071.6 5 96627325 - Fras1 NNNAAAGAGAGATGTAGCCT NGG 1 0.4706 Tier II
7 NC_000076.6 10 122932584 + Ppm1h NNNAAGCAGAGATGTAGTCT NGG 2 0.4592 Tier II
8 NC_000067.6 1 192993522 - Syt14 NNNAAACAGAGGTGAAGCCT NGG 2 0.4181 Tier II
9 NC_000068.7 2 162399122 + Ptprt NNNAAGCAGAGATGTAGCCA NGG 2 0.4018 Tier II
10 NC_000067.6 1 164363438 + Nme7 NNNAGACAGAGATGTAGACT NGG 2 0.3877 Tier II
11 NC_000084.6 18 44686753 - Mcc NNNAAATAGAGATGTAGCTT NGG 2 0.375 Tier II
12 NC_000070.6 4 126298662 + Col8a2 NNNCAACAGAGATGTAGCCT NGG 1 0.3529 Tier II
13 NC_000081.6 15 3475263 + Ghr NNNAAACAGAATTGTAGCCT NGG 2 0.3333 Tier II
14 NC_000075.6 9 90183104 + Adamts7 NNNAACCAGAGAAGTAGCCT NGG 2 0.3147 Tier II
15 NC_000077.6 11 68287891 - Ntn1 NNNAGACAGAGATGCAGCCT NGG 2 0.1964 Tier II
16 NC_000071.6 5 37392714 - Evc2 NNNAAGCAGAGCTGTAGCCT NGG 2 0.188 Tier II
17 NC_000084.6 18 78119524 - Slc14a1 NNNAGACAGAGATGTAGGCT NGG 2 0.096 Tier II
18 NC_000074.6 8 23681173 - Zmat4 NNNAAACAGAGCTGTATCCT NGG 2 0.0658 Tier II
19 NC_000078.6 12 98752687 - Zc3h14 NNNAAACAGAGAAGTAGCCT NGA 2 0.0481 Tier II
20 NC_000072.6 6 55122547 + Crhr2 NNNAAACAGAGATGTAGGCT NAG 2 0.0346 Tier II
21 NC_000084.6 18 63904464 + Wdr7 NNNAAACTGAGATGTAGCCT NGA 2 0.0298 Tier II
22 NC_000082.6 16 44348718 - Spice1 NNNAAACAGAGAAGTAGCCT NTG 2 0.027 Tier II
23 NC_000076.6 10 24709010 - Enpp1 NNNAAACACAGATGGAGCCT NGG 2 0.0269 Tier II
24 NC_000080.6 14 67881814 + Dock5 NNNAAACATAGATGTAGCCT NTG 2 0.0208 Tier II
25 NC_000083.6 17 65407750 - Tmem232 NNNAAACATAGATGTAGCCT NTG 2 0.0208 Tier II
26 NC_000083.6 17 28706439 + Mapk14 NNNATACAGAGATGGAGCCT NGG 2 0.0182 Tier II
27 NC_000083.6 17 67884337 + Arhgap28 NNNCAACAGAGATGGAGCCT NGG 2 0.0176 Tier II
28 NC_000079.6 13 76065984 + Arsk NNNAAAAAGAGATGTAGCCT NGT 2 0.0121 Tier II
29 NC_000077.6 11 46704197 + Timd2 NNNAACCAGAGATGTAGCCT NGT 2 0.0073 Tier II
30 NC_000068.7 2 128705649 + Mertk NNNAAACAGAGATGTTGCCT NTG 2 0.0 Tier II
31 NC_000076.6 10 66149744 - 1700023F02Rik NNNAAACTGAGATGTAGCCT NGG 1 0.4286 Tier III
32 NC_000076.6 10 66149744 - 4930407I19Rik NNNAAACTGAGATGTAGCCT NGG 1 0.4286 Tier III
33 NC_000073.6 7 69800636 + Gm35209 NNNAAACAGAAATTTAGCCT NGG 2 0.2667 Tier III
34 NC_000071.6 5 97345242 + Gm33969 NNNAATCAGAGATGCAGCCT NGG 2 0.1948 Tier III
35 NC_000085.6 19 28692108 - D930032P07Rik NNNAAACAGAGATGTGGTCT NGG 2 0.1236 Tier III
36 NC_000073.6 7 71159495 - Gm36071 NNNAAACACAGATGGAGCCT NGG 2 0.0269 Tier III
37 NC_000085.6 19 10762716 - A430093F15Rik NNNAAACACAGATGGAGCCT NGG 2 0.0269 Tier III
38 NC_000068.7 2 128187581 + Gm14009 NNNAAACAGAGATGTATCCC NGG 2 0.0227 Tier III
39 NC_000079.6 13 76065984 + Gm41006 NNNAAAAAGAGATGTAGCCT NGT 2 0.0121 Tier III
40 NC_000068.7 2 136604902 - Gm32659 NNNAAACAGAGATGTAGGCT NGT 2 0.0022 Tier III
Download CSV

Other clones with same target sequence:

(none)