Construct: sgRNA BRDN0001147775

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GACTGGATGATGAAGTGCCG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FES (2242)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76507

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000015.10 15 90891168 - FES NNNTGGATGATGAAGTGCCG NGG 0 1.0 Tier I
2 NC_000009.12 9 34372515 + MYORG NNNTGGATGAAGAAGTGCAG NGG 2 0.3214 Tier I
3 NC_000019.10 19 55669183 - U2AF2 NNNTGGAAGATGACGTGCCG NGG 2 0.1714 Tier I
4 NC_000017.11 17 8758287 - SPDYE4 NNNTGGATGATGAAGTGCCA NGA 2 0.0651 Tier I
5 NC_000007.14 7 73027850 - SPDYE8 NNNTGGATGATGAAGTGTCG NGA 2 0.0446 Tier I
6 NC_000007.14 7 73055995 - SPDYE11 NNNTGGATGATGAAGTGTCG NGA 2 0.0446 Tier I
7 NC_000007.14 7 73084122 - SPDYE9 NNNTGGATGATGAAGTGTCG NGA 2 0.0446 Tier I
8 NC_000007.14 7 73112136 - SPDYE10 NNNTGGATGATGAAGTGTCG NGA 2 0.0446 Tier I
9 NC_000007.14 7 74913164 - SPDYE12 NNNTGGATGATGAAGTGTCG NGA 2 0.0446 Tier I
10 NC_000007.14 7 77030454 - SPDYE17 NNNTGGATGATGAAGTGTCG NGA 2 0.0446 Tier I
11 NC_000007.14 7 100307979 + SPDYE3 NNNTGGATGATGAAGTGTCG NGA 2 0.0446 Tier I
12 NC_000023.11 X 45000438 + KDM6A NNNTGGTTGATGAAGTGCCA NGG 2 0.4102 Tier II
13 NC_000011.10 11 64096470 + FLRT1 NNNAGGATGATGAAGTGCAG NGG 2 0.2041 Tier II
14 NC_000011.10 11 64096470 + MACROD1 NNNAGGATGATGAAGTGCAG NGG 2 0.2041 Tier II
15 NC_000002.12 2 85873404 + ST3GAL5 NNNTGGATGATGAAGGGCCC NGG 2 0.1484 Tier II
16 NC_000001.11 1 225253921 - DNAH14 NNNTGGATGATGAAGTGCCT NGA 2 0.0486 Tier II
17 NC_000017.11 17 59182762 - PRR11 NNNTGGATGATGAAGTGTCG NGA 2 0.0446 Tier II
18 NC_000009.12 9 99741915 + LOC101928438 NNNTGGATGATGAAGAGCCA NGG 2 0.8523 Tier III
19 NC_000009.12 9 99741915 + LOC124902234 NNNTGGATGATGAAGAGCCA NGG 2 0.8523 Tier III
20 NC_000003.12 3 54075321 - LOC124909381 NNNTGGTTGATGAAATGCCG NGG 2 0.4118 Tier III
21 NC_000010.11 10 84169458 + CERNA2 NNNTGGTTGATGAAGTGCCA NGG 2 0.4102 Tier III
22 NC_000004.12 4 11592773 - LOC107986178 NNNTTGATGATGAAGTGCCG NAG 2 0.0778 Tier III
23 NC_000007.14 7 6721930 - SPDYE20P NNNTGGATGATGAAGTGTCG NGA 2 0.0446 Tier III
24 NC_000007.14 7 74913164 - PMS2P5 NNNTGGATGATGAAGTGTCG NGA 2 0.0446 Tier III
25 NC_000017.11 17 59182762 - SPDYE22P NNNTGGATGATGAAGTGTCG NGA 2 0.0446 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000073.6 7 80381625 + Fes NNNTGGATGATGAAATGTCG NGG 2 0.605 Tier I
2 NC_000075.6 9 44506211 + Bcl9l NNNTGGAGCATGAAGTGCCG NGG 2 0.3949 Tier I
3 NC_000080.6 14 77935398 + Epsti1 NNNTGGATGTTGAAGTGCAG NGG 2 0.3782 Tier II
4 NC_000070.6 4 15071248 - Necab1 NNNTGGAGGATGAAGTGCCC NGG 2 0.3143 Tier II
5 NC_000067.6 1 139283757 - Crb1 NNNTGGATGATGCAGTGCCA NGG 2 0.1974 Tier II
6 NC_000071.6 5 13384748 - Sema3a NNNTGGGTGATGAAGTGCCG NAG 2 0.183 Tier II
7 NC_000078.6 12 71563646 + Gm40439 NNNTGGATAATGGAGTGCCG NGG 2 0.4193 Tier III
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Other clones with same target sequence:

(none)