Construct: sgRNA BRDN0001147777
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGAAGAATTCCATCCACGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LIMK1 (3984)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76049
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
74105920 |
+ |
LIMK1 |
NNNAAGAATTCCATCCACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
4686708 |
- |
AJAP1 |
NNNAAGAGCTCCATCCACGT |
NGG |
2 |
0.6769 |
Tier II |
| 3 |
NC_000010.11 |
10 |
30871312 |
+ |
ZNF438 |
NNNTAGAATTCCATCCACTT |
NGG |
2 |
0.4242 |
Tier II |
| 4 |
NC_000005.10 |
5 |
173939058 |
- |
CPEB4 |
NNNAAGAAATCCATCCACCT |
NGG |
2 |
0.4163 |
Tier II |
| 5 |
NC_000007.14 |
7 |
36979964 |
+ |
ELMO1 |
NNNAAGAATTCCAACCAAGT |
NGG |
2 |
0.3333 |
Tier II |
| 6 |
NC_000005.10 |
5 |
151204283 |
+ |
CCDC69 |
NNNAAGCATTCCATCCACCT |
NGG |
2 |
0.1961 |
Tier II |
| 7 |
NC_000003.12 |
3 |
53696709 |
- |
CACNA1D |
NNNCAGAATTCCATCCACCT |
NGG |
2 |
0.1582 |
Tier II |
| 8 |
NC_000007.14 |
7 |
39956170 |
- |
CDK13 |
NNNAAGAATTCCATTCACCT |
NGG |
2 |
0.0299 |
Tier II |
| 9 |
NC_000006.12 |
6 |
2625966 |
+ |
LINC01600 |
NNNGAGAATTCCATCCAAGT |
NGG |
2 |
0.3365 |
Tier III |
| 10 |
NC_000005.10 |
5 |
142374155 |
+ |
SPRY4-AS1 |
NNNAAGTATTCCATCCACCT |
NGG |
2 |
0.1961 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
134669251 |
- |
Limk1 |
NNNAAGAATTCCATCCACGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000073.6 |
7 |
123363026 |
- |
Arhgap17 |
NNNAACAATTCCATCCATGT |
NGG |
2 |
0.4383 |
Tier II |
| 3 |
NC_000077.6 |
11 |
43172567 |
+ |
Atp10b |
NNNAAGAATGCCATCCACAT |
NGG |
2 |
0.3571 |
Tier II |
| 4 |
NC_000083.6 |
17 |
45544597 |
- |
Tmem151b |
NNNAAGAATCCCATCCACCT |
NGG |
2 |
0.2391 |
Tier II |
| 5 |
NC_000073.6 |
7 |
139011900 |
+ |
Jakmip3 |
NNNAAGAACTCCATCCAGGT |
NGG |
2 |
0.1231 |
Tier II |
| 6 |
NC_000075.6 |
9 |
107511178 |
+ |
Cacna2d2 |
NNNAAGAATGCCATCCAGGT |
NGG |
2 |
0.0667 |
Tier II |
| 7 |
NC_000075.6 |
9 |
102340210 |
+ |
Ephb1 |
NNNAAGAATTACAGCCACGT |
NGG |
2 |
0.0 |
Tier II |
| 8 |
NC_000076.6 |
10 |
118232983 |
- |
Gm9585 |
NNNAAGAATTCCATCCATGT |
NGG |
1 |
0.6429 |
Tier III |
| 9 |
NC_000076.6 |
10 |
118266486 |
+ |
Gm9035 |
NNNAAGAATTCCATCCATGT |
NGG |
1 |
0.6429 |
Tier III |
Other clones with same target sequence:
(none)
