Construct: sgRNA BRDN0001147781

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ATTACACTGTTGGAGTGTGT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
ACVR1 (90)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76758

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000002.12 2 157774093 - ACVR1 NNNACACTGTTGGAGTGTGT NGG 0 1.0 Tier I
2 NC_000016.10 16 13112440 + SHISA9 NNNACACTGTTGGAGTATAT NGG 2 0.6667 Tier II
3 NC_000005.10 5 175870907 - CPLX2 NNNACACTGATGGAGCGTGT NGG 2 0.5714 Tier II
4 NC_000005.10 5 7540565 + ADCY2 NNNACACTGTTGAAGTGTCT NGG 2 0.4138 Tier II
5 NC_000016.10 16 27209750 + KDM8 NNNACACTGTTGGAGAGTCT NGG 2 0.4075 Tier II
6 NC_000012.12 12 109318673 - MYO1H NNNACAATTTTGGAGTGTGT NGG 2 0.4 Tier II
7 NC_000022.11 22 31082036 - SMTN NNNACACAGTTGGGGTGTGT NGG 2 0.3733 Tier II
8 NC_000014.9 14 33374696 + NPAS3 NNNACAATGTTGGGGTGTGT NGG 2 0.35 Tier II
9 NC_000006.12 6 20544883 + CDKAL1 NNNACACTAGTGGAGTGTGT NGG 2 0.3214 Tier II
10 NC_000002.12 2 201176403 + CFLAR NNNACACTCTTGGTGTGTGT NGG 2 0.2872 Tier II
11 NC_000018.10 18 69761410 + DOK6 NNNACACTGCTGGTGTGTGT NGG 2 0.2844 Tier II
12 NC_000001.11 1 176821923 - PAPPA2 NNNAGACTGTTGGAGTGTCT NGG 2 0.269 Tier II
13 NC_000007.14 7 48041295 - C7orf57 NNNACACTGTTGCTGTGTGT NGG 2 0.2246 Tier II
14 NC_000001.11 1 114407792 + TRIM33 NNNTCACTGTTGGAGGGTGT NGG 2 0.2203 Tier II
15 NC_000008.11 8 130853378 + ADCY8 NNNACACTGGTGGAGTGCGT NGG 2 0.2143 Tier II
16 NC_000012.12 12 221719 - SLC6A13 NNNACACTGCTGGAGGGTGT NGG 2 0.1846 Tier II
17 NC_000011.10 11 117065684 + SIK3 NNNACACTGTTGGAGTGTAG NGG 2 0.1261 Tier II
18 NC_000014.9 14 102993278 - CDC42BPB NNNACACTGGTGGAGTCTGT NGG 2 0.1176 Tier II
19 NC_000016.10 16 12943214 + SHISA9 NNNCCACTGTTGGAGTGGGT NGG 2 0.1176 Tier II
20 NC_000005.10 5 60977155 + NDUFAF2 NNNACACTGGTGGAGTGTGG NGG 2 0.0882 Tier II
21 NC_000016.10 16 14565589 - PARN NNNAGACTGTTGGATTGTGT NGG 2 0.0857 Tier II
22 NC_000011.10 11 89280456 + TYR NNNACACTGTTGGAGTGTTT NTG 2 0.026 Tier II
23 NC_000005.10 5 150263548 - CAMK2A NNNACAGTGTTGGAGTGTGT NTG 2 0.0183 Tier II
24 NC_000006.12 6 91672481 + CASC6 NNNACACTATTGGAATGTGT NGG 2 0.605 Tier III
25 NC_000018.10 18 39630257 - MIR924HG NNNACACTGTAGGGGTGTGT NGG 2 0.35 Tier III
26 NC_000001.11 1 70455832 + LOC105378793 NNNAAAGTGTTGGAGTGTGT NGG 2 0.2689 Tier III
27 NC_000008.11 8 130853378 + LOC105375762 NNNACACTGGTGGAGTGCGT NGG 2 0.2143 Tier III
28 NC_000016.10 16 12943214 + LOC105371092 NNNCCACTGTTGGAGTGGGT NGG 2 0.1176 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 58474178 - Acvr1 NNNACCCTGTTGGAGTGTGT NGG 1 0.4545 Tier I
2 NC_000073.6 7 27343362 - Shkbp1 NNNACAGTGTTGGAGTGTGA NGG 2 0.2647 Tier I
3 NC_000078.6 12 51928056 - Heatr5a NNNACACTGTAAGAGTGTGT NGG 2 0.7 Tier II
4 NC_000080.6 14 18609394 + Ube2e2 NNNTCACTGTTGAAGTGTGT NGG 2 0.5874 Tier II
5 NC_000071.6 5 93152179 + Septin11 NNNAAACTGTTGAAGTGTGT NGG 2 0.5275 Tier II
6 NC_000075.6 9 118789193 + Itga9 NNNAAAATGTTGGAGTGTGT NGG 2 0.4286 Tier II
7 NC_000068.7 2 68354548 - Stk39 NNNACACTGTTGGAGTGAGA NGG 2 0.375 Tier II
8 NC_000084.6 18 11702047 + Rbbp8 NNNACCTTGTTGGAGTGTGT NGG 2 0.3693 Tier II
9 NC_000068.7 2 179964444 + Taf4 NNNACAGTGTAGGAGTGTGT NGG 2 0.3529 Tier II
10 NC_000072.6 6 28052741 - Grm8 NNNTCACTTTTGGAGTGTGT NGG 2 0.3394 Tier II
11 NC_000080.6 14 65726177 + Scara5 NNNACACTGATGGAGGGTGT NGG 2 0.2967 Tier II
12 NC_000085.6 19 45650187 + Fbxw4 NNNACACTTTTGGTGTGTGT NGG 2 0.2844 Tier II
13 NC_000079.6 13 74105855 - Slc9a3 NNNATACTGTGGGAGTGTGT NGG 2 0.2545 Tier II
14 NC_000077.6 11 36490106 - Tenm2 NNNACACTGTCGGAGGGTGT NGG 2 0.2308 Tier II
15 NC_000067.6 1 15644899 + Kcnb2 NNNCCACTGCTGGAGTGTGT NGG 2 0.1882 Tier II
16 NC_000084.6 18 25732690 - Celf4 NNNACACTGTTGGGGGGTGT NGG 2 0.1615 Tier II
17 NC_000069.6 3 80882817 - Glrb NNNACACTGTTGCAGGGTGT NGG 2 0.1457 Tier II
18 NC_000068.7 2 41966345 - Lrp1b NNNACACTGATGGATTGTGT NGG 2 0.1224 Tier II
19 NC_000082.6 16 96949303 + Dscam NNNACACTGTGGGAGTCTGT NGG 2 0.0941 Tier II
20 NC_000069.6 3 90604960 + S100a4 NNNACACTGTTGGCGTGGGT NGG 2 0.0714 Tier II
21 NC_000069.6 3 31994245 - Kcnmb2 NNNACACTGTGGGAGTGTGG NGG 2 0.0706 Tier II
22 NC_000076.6 10 27609282 - Lama2 NNNACACTGTGGGAGTGTGG NGG 2 0.0706 Tier II
23 NC_000078.6 12 83897573 - Numb NNNACACTGTTGCAGTGTGT NCG 2 0.0451 Tier II
24 NC_000079.6 13 24831055 - Acot13 NNNAAACTGTTGGAGTGTGT NGA 2 0.0397 Tier II
25 NC_000077.6 11 118169723 + Cyth1 NNNACACTGTTGGTGTGTGT NTG 2 0.0208 Tier II
26 NC_000071.6 5 69125241 + Kctd8 NNNACACTGTGGGAGTGTGT NTG 2 0.0156 Tier II
27 NC_000072.6 6 94677758 - Lrig1 NNNACACTGTTGAAGTGTGT NGT 2 0.0149 Tier II
28 NC_000080.6 14 78963965 + Vwa8 NNNACACTGTTGCAGTGTGT NGT 2 0.0068 Tier II
29 NC_000073.6 7 88349398 - Gm2407 NNNTCACTGTTGGAGAGTGT NGG 2 0.5785 Tier III
30 NC_000071.6 5 119081902 + Gm31034 NNNACACTGTTGGAGGGTTT NGG 2 0.2308 Tier III
31 NC_000071.6 5 101710591 - 9430085M18Rik NNNACCCTGTTGGGGTGTGT NGG 2 0.2121 Tier III
32 NC_000077.6 11 96926039 + Gm11525 NNNACACTGTTGGCTTGTGT NGG 2 0.0306 Tier III
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Other clones with same target sequence:

(none)