Construct: sgRNA BRDN0001147784
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTGAAGAAGGAATTTGGAGG
Original Target:
- Taxon:
- Homo sapiens (human)
- Gene:
- TWF1 (5756)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000012.12 | 12 | 43800475 | - | TWF1 | NNNAAGAAGGAATTTGGAGG | NGG | 0 | 1.0 | Tier I |
2 | NC_000003.12 | 3 | 52231484 | - | TWF2 | NNNAAAAAGGAGTTTGGAGG | NGG | 2 | 0.7222 | Tier I |
3 | NC_000011.10 | 11 | 46382460 | + | MDK | NNNAAGAAGGAGTTTGGAGG | NGA | 2 | 0.0502 | Tier I |
4 | NC_000006.12 | 6 | 41071158 | - | OARD1 | NNNAAGAAGAAATTTGGAGG | NGT | 2 | 0.0151 | Tier I |
5 | NC_000012.12 | 12 | 43800475 | - | TMEM117 | NNNAAGAAGGAATTTGGAGG | NGG | 0 | 1.0 | Tier II |
6 | NC_000004.12 | 4 | 184428363 | + | IRF2 | NNNAAAAAGGAATTTGGAGA | NGG | 2 | 0.9375 | Tier II |
7 | NC_000010.11 | 10 | 84499531 | - | CCSER2 | NNNAAGAAGGAATTTAAAGG | NGG | 2 | 0.9333 | Tier II |
8 | NC_000016.10 | 16 | 1578358 | + | IFT140 | NNNAAGAAGGAACTTGGAGA | NGG | 2 | 0.7401 | Tier II |
9 | NC_000002.12 | 2 | 195629033 | - | SLC39A10 | NNNAAGGAGAAATTTGGAGG | NGG | 2 | 0.6588 | Tier II |
10 | NC_000017.11 | 17 | 58975894 | + | TRIM37 | NNNAAAAAGGAATATGGAGG | NGG | 2 | 0.619 | Tier II |
11 | NC_000017.11 | 17 | 58975894 | + | PPM1E | NNNAAAAAGGAATATGGAGG | NGG | 2 | 0.619 | Tier II |
12 | NC_000010.11 | 10 | 34731718 | - | PARD3 | NNNAAGAAGGAATTAGGAGA | NGG | 2 | 0.5428 | Tier II |
13 | NC_000002.12 | 2 | 60484685 | + | BCL11A | NNNAAGGGGGAATTTGGAGG | NGG | 2 | 0.5176 | Tier II |
14 | NC_000019.10 | 19 | 18940042 | + | HOMER3 | NNNAAGGAGGAGTTTGGAGG | NGG | 2 | 0.5098 | Tier II |
15 | NC_000001.11 | 1 | 155352690 | + | ASH1L | NNNAAGAACGAATTTGAAGG | NGG | 2 | 0.5026 | Tier II |
16 | NC_000006.12 | 6 | 35719354 | + | FKBP5 | NNNACGAAGGAATTTGGAGG | NGG | 1 | 0.5 | Tier II |
17 | NC_000001.11 | 1 | 198267416 | + | NEK7 | NNNAATAAGGAGTTTGGAGG | NGG | 2 | 0.4815 | Tier II |
18 | NC_000001.11 | 1 | 70890526 | - | PTGER3 | NNNAAGAAAGAATTTGGAAG | NGG | 2 | 0.4592 | Tier II |
19 | NC_000020.11 | 20 | 46033668 | - | SLC12A5 | NNNGAGAAGGAGTTTGGAGG | NGG | 2 | 0.4514 | Tier II |
20 | NC_000005.10 | 5 | 149938837 | + | PDE6A | NNNAAGTAGGAATTTGGAGG | NGG | 1 | 0.4375 | Tier II |
21 | NC_000002.12 | 2 | 101134125 | + | TBC1D8 | NNNAACAATGAATTTGGAGG | NGG | 2 | 0.3636 | Tier II |
22 | NC_000010.11 | 10 | 131210725 | - | TCERG1L | NNNAAGAAGGAATAAGGAGG | NGG | 2 | 0.3584 | Tier II |
23 | NC_000019.10 | 19 | 13063736 | - | NFIX | NNNAAGCAGGAGTTTGGAGG | NGG | 2 | 0.316 | Tier II |
24 | NC_000019.10 | 19 | 14706353 | - | ZNF333 | NNNAAGATGGAGTTTGGAGG | NGG | 2 | 0.3095 | Tier II |
25 | NC_000012.12 | 12 | 106325461 | + | TCP11L2 | NNNAAGAAGGTATTTGGAGA | NGG | 2 | 0.2885 | Tier II |
26 | NC_000014.9 | 14 | 57937737 | + | SLC35F4 | NNNATGAAGGAACTTGGAGG | NGG | 2 | 0.2871 | Tier II |
27 | NC_000007.14 | 7 | 29848781 | + | WIPF3 | NNNAAGCAGGGATTTGGAGG | NGG | 2 | 0.2844 | Tier II |
28 | NC_000002.12 | 2 | 237759614 | - | LRRFIP1 | NNNAAGAAGGAATTCAGAGG | NGG | 2 | 0.2727 | Tier II |
29 | NC_000005.10 | 5 | 80424458 | - | ZFYVE16 | NNNAAAAAGGAATTTGGAGG | NAG | 2 | 0.2593 | Tier II |
30 | NC_000003.12 | 3 | 127733440 | - | MGLL | NNNAAGAAGGAATTTAGAGG | NAG | 2 | 0.2593 | Tier II |
31 | NC_000016.10 | 16 | 76290569 | - | CNTNAP4 | NNNAAGAAGGAATTTAGAGG | NAG | 2 | 0.2593 | Tier II |
32 | NC_000002.12 | 2 | 224027893 | - | SERPINE2 | NNNAAGAAGGAAGTTGAAGG | NGG | 2 | 0.2435 | Tier II |
33 | NC_000008.11 | 8 | 13107656 | + | DLC1 | NNNAAGAAGGTATTTGGAAG | NGG | 2 | 0.2198 | Tier II |
34 | NC_000002.12 | 2 | 213041924 | + | IKZF2 | NNNAAGAAGTAATTTGGAGG | NAG | 2 | 0.2106 | Tier II |
35 | NC_000008.11 | 8 | 135505696 | + | KHDRBS3 | NNNAAGAAGTAATTTGGAGG | NAG | 2 | 0.2106 | Tier II |
36 | NC_000011.10 | 11 | 28040475 | + | KIF18A | NNNAAGAAGGAACTTGGAGG | NAG | 2 | 0.2047 | Tier II |
37 | NC_000002.12 | 2 | 84824236 | + | TRABD2A | NNNGAGAAGGTATTTGGAGG | NGG | 2 | 0.1923 | Tier II |
38 | NC_000003.12 | 3 | 62669510 | - | CADPS | NNNAGGAAGGAATTTGGAGG | NAG | 2 | 0.1867 | Tier II |
39 | NC_000008.11 | 8 | 19939617 | + | LPL | NNNAAGAAGGAAGTTGGAAG | NGG | 2 | 0.1863 | Tier II |
40 | NC_000014.9 | 14 | 68410783 | + | RAD51B | NNNAAGATGCAATTTGGAGG | NGG | 2 | 0.1714 | Tier II |
41 | NC_000012.12 | 12 | 109241369 | + | ACACB | NNNAAGATGGCATTTGGAGG | NGG | 2 | 0.1714 | Tier II |
42 | NC_000010.11 | 10 | 19391506 | - | MALRD1 | NNNAAGAAAGAATTTGGAGG | NAG | 2 | 0.1667 | Tier II |
43 | NC_000002.12 | 2 | 158798256 | + | DAPL1 | NNNGAGAAGGAAGTTGGAGG | NGG | 2 | 0.163 | Tier II |
44 | NC_000003.12 | 3 | 140574199 | - | CLSTN2 | NNNGAGAAGGAAGTTGGAGG | NGG | 2 | 0.163 | Tier II |
45 | NC_000020.11 | 20 | 37345165 | + | SRC | NNNAAGAAGGAATATGGAGG | NAG | 2 | 0.1605 | Tier II |
46 | NC_000016.10 | 16 | 88321882 | - | ZNF469 | NNNAAGAAGGCATTTGGGGG | NGG | 2 | 0.16 | Tier II |
47 | NC_000003.12 | 3 | 57848493 | - | SLMAP | NNNAAGAAGGAAGTAGGAGG | NGG | 2 | 0.151 | Tier II |
48 | NC_000011.10 | 11 | 67010611 | - | SYT12 | NNNCAGAAGGCATTTGGAGG | NGG | 2 | 0.1412 | Tier II |
49 | NC_000014.9 | 14 | 83963378 | - | LOC124903401 | NNNAAGACGGTATTTGGAGG | NGG | 2 | 0.1319 | Tier II |
50 | NC_000020.11 | 20 | 9363063 | - | PLCB4 | NNNAAGCAGGAATTTGGAGG | NAG | 2 | 0.1134 | Tier II |
51 | NC_000013.11 | 13 | 41600838 | - | VWA8 | NNNAAGAAGCAATTTGGAGG | NAG | 2 | 0.1037 | Tier II |
52 | NC_000012.12 | 12 | 234864 | - | SLC6A13 | NNNAAGAAGGAATTTGGGGG | NAG | 2 | 0.1037 | Tier II |
53 | NC_000007.14 | 7 | 86972446 | - | ELAPOR2 | NNNAAAAAGGAATTTGGAGG | NGA | 2 | 0.0694 | Tier II |
54 | NC_000005.10 | 5 | 32749631 | - | NPR3 | NNNAAGAAGGAATTTGGAGG | NGA | 1 | 0.0694 | Tier II |
55 | NC_000002.12 | 2 | 187377993 | + | CALCRL | NNNAAGAAGGAAGTTGGAGG | NAG | 2 | 0.0676 | Tier II |
56 | NC_000004.12 | 4 | 21601160 | - | KCNIP4 | NNNAAGAAGGAATTGAGAGG | NGG | 2 | 0.05 | Tier II |
57 | NC_000003.12 | 3 | 175178810 | + | NAALADL2 | NNNAAGAAGGAATTTGGAAG | NGA | 2 | 0.0496 | Tier II |
58 | NC_000005.10 | 5 | 150588906 | - | SYNPO | NNNAAGAAGGAATTGGGAGA | NGG | 2 | 0.0469 | Tier II |
59 | NC_000011.10 | 11 | 12715208 | - | TEAD1 | NNNAATAAGGAATTTGGAGG | NGA | 2 | 0.0463 | Tier II |
60 | NC_000019.10 | 19 | 13382450 | + | CACNA1A | NNNAAGAAGGAATTAGGAGG | NGA | 2 | 0.0402 | Tier II |
61 | NC_000011.10 | 11 | 4826771 | + | MMP26 | NNNAAAAAGGAATTTGGAGG | NTG | 2 | 0.039 | Tier II |
62 | NC_000008.11 | 8 | 109631626 | + | SYBU | NNNAAGCAGGAATTTGGAGG | NGA | 2 | 0.0304 | Tier II |
63 | NC_000002.12 | 2 | 168243916 | - | STK39 | NNNAAGAAGGAATTTGGGGG | NGA | 2 | 0.0278 | Tier II |
64 | NC_000008.11 | 8 | 95074904 | - | NDUFAF6 | NNNAAGAAGGAATTTGGAGT | NTG | 2 | 0.0273 | Tier II |
65 | NC_000001.11 | 1 | 152510965 | + | LCE5A | NNNAAGAAGGATTTTGGAGG | NGA | 2 | 0.0231 | Tier II |
66 | NC_000015.10 | 15 | 99147526 | - | TTC23 | NNNAAGAATGAATTTGGAGG | NTG | 2 | 0.0208 | Tier II |
67 | NC_000003.12 | 3 | 136878957 | + | NCK1 | NNNAAGTAGGAATTTGGAGG | NTG | 2 | 0.017 | Tier II |
68 | NC_000007.14 | 7 | 147894060 | - | CNTNAP2 | NNNGAGAAGGAATTTGGAGG | NGC | 2 | 0.0139 | Tier II |
69 | NC_000002.12 | 2 | 19665271 | - | LOC124905977 | NNNAAGAAGGAATTTGGTGG | NGT | 2 | 0.0081 | Tier II |
70 | NC_000022.11 | 22 | 25166512 | - | KIAA1671 | NNNAAGAAGGAATTTGCAGG | NGC | 2 | 0.0052 | Tier II |
71 | NC_000002.12 | 2 | 80524298 | - | CTNNA2 | NNNAACAAGGAATGTGGAGG | NGG | 2 | 0.0 | Tier II |
72 | NC_000023.11 | X | 24202505 | - | ZFX | NNNAAGAAGGAACGTGGAGG | NGG | 2 | 0.0 | Tier II |
73 | NC_000005.10 | 5 | 113228580 | + | MCC | NNNAAGAAGGAAGGTGGAGG | NGG | 2 | 0.0 | Tier II |
74 | NC_000009.12 | 9 | 98387849 | - | GABBR2 | NNNAAGAAGGAATTTGGAGG | NTC | 2 | 0.0 | Tier II |
75 | NC_000004.12 | 4 | 82640529 | + | SCD5 | NNNAAGAAGGAATTTTTAGG | NGG | 2 | 0.0 | Tier II |
76 | NC_000005.10 | 5 | 139676218 | - | CXXC5 | NNNAAGAAGGACTGTGGAGG | NGG | 2 | 0.0 | Tier II |
77 | NC_000005.10 | 5 | 139676254 | - | CXXC5 | NNNAAGAAGGACTGTGGAGG | NGG | 2 | 0.0 | Tier II |
78 | NC_000002.12 | 2 | 235562617 | - | AGAP1 | NNNAAGAAGGCATGTGGAGG | NGG | 2 | 0.0 | Tier II |
79 | NC_000004.12 | 4 | 186701681 | - | FAT1 | NNNGAGAAGGAATGTGGAGG | NGG | 2 | 0.0 | Tier II |
80 | NC_000017.11 | 17 | 29204137 | - | TWF1P1 | NNNAAGAAGGAATTTGGAGG | NGG | 0 | 1.0 | Tier III |
81 | NC_000002.12 | 2 | 191194771 | - | LOC105373804 | NNNAGAAAGGAATTTGGAGG | NGG | 2 | 0.72 | Tier III |
82 | NC_000008.11 | 8 | 55064254 | - | LOC107986886 | NNNAAGAAGGGATTTGGAGA | NGG | 2 | 0.6094 | Tier III |
83 | NC_000008.11 | 8 | 55064254 | - | LOC107986887 | NNNAAGAAGGGATTTGGAGA | NGG | 2 | 0.6094 | Tier III |
84 | NC_000003.12 | 3 | 178031625 | + | LOC105374234 | NNNAAGAAAGAATTTGGAAG | NGG | 2 | 0.4592 | Tier III |
85 | NC_000006.12 | 6 | 40992628 | - | LOC101929555 | NNNGAGAAGGAAATTGGAGG | NGG | 2 | 0.4327 | Tier III |
86 | NC_000006.12 | 6 | 90829152 | + | LOC107986623 | NNNAAGAAGGAAAATGGAGG | NGG | 2 | 0.4286 | Tier III |
87 | NC_000010.11 | 10 | 120302485 | + | LOC105378515 | NNNGAGAAGGGATTTGGAGG | NGG | 2 | 0.4063 | Tier III |
88 | NC_000002.12 | 2 | 84300359 | - | LOC107985905 | NNNAAGGAGGAATTTGGTGG | NGG | 2 | 0.3529 | Tier III |
89 | NC_000002.12 | 2 | 84300446 | - | LOC107985905 | NNNAAGGAGGAATTTGGTGG | NGG | 2 | 0.3529 | Tier III |
90 | NC_000002.12 | 2 | 84300533 | - | LOC107985905 | NNNAAGGAGGAATTTGGTGG | NGG | 2 | 0.3529 | Tier III |
91 | NC_000001.11 | 1 | 168914661 | - | LINC00970 | NNNGAGACGGAATTTGGAGG | NGG | 2 | 0.2679 | Tier III |
92 | NC_000005.10 | 5 | 177560410 | - | FAM193B-DT | NNNAAGAAAGAATTTGGGGG | NGG | 2 | 0.2571 | Tier III |
93 | NC_000005.10 | 5 | 38644401 | + | LIFR-AS1 | NNNGAGAAGCAATTTGGAGG | NGG | 2 | 0.25 | Tier III |
94 | NC_000007.14 | 7 | 149870770 | - | ATP6V0E2-AS1 | NNNAAGAAGGAATTAGGAGC | NGG | 2 | 0.2481 | Tier III |
95 | NC_000010.11 | 10 | 84294417 | + | LINC00858 | NNNATGAAGGAATTAGGAGG | NGG | 2 | 0.2105 | Tier III |
96 | NC_000006.12 | 6 | 134876274 | + | LOC101928277 | NNNAAGAAGGGATTTGGAGG | NAG | 2 | 0.1685 | Tier III |
97 | NC_000020.11 | 20 | 48555820 | - | LOC105372646 | NNNAAGAAGCAATTTGGAGG | NAG | 2 | 0.1037 | Tier III |
98 | NC_000002.12 | 2 | 187377993 | + | CALCRL-AS1 | NNNAAGAAGGAAGTTGGAGG | NAG | 2 | 0.0676 | Tier III |
99 | NC_000004.12 | 4 | 21601160 | - | LOC105374516 | NNNAAGAAGGAATTGAGAGG | NGG | 2 | 0.05 | Tier III |
100 | NC_000006.12 | 6 | 128692408 | - | LOC105377998 | NNNAAGAAGGAATTTGGAGT | NGA | 2 | 0.0486 | Tier III |
101 | NC_000001.11 | 1 | 20399266 | + | LINC01141 | NNNAAGAAGGAATTTGGAGA | NGC | 2 | 0.0208 | Tier III |
102 | NC_000003.12 | 3 | 87136239 | - | LINC00506 | NNNAAGAATGAATTTGGAGG | NTG | 2 | 0.0208 | Tier III |
103 | NC_000003.12 | 3 | 184559293 | - | LOC124909469 | NNNAAGAAGGACTTGGGAGG | NGG | 2 | 0.0132 | Tier III |
104 | NC_000015.10 | 15 | 26121608 | - | LINC00929 | NNNAAGAAGGAAAGTGGAGG | NGG | 2 | 0.0 | Tier III |
105 | NC_000015.10 | 15 | 26121608 | - | LOC105370739 | NNNAAGAAGGAAAGTGGAGG | NGG | 2 | 0.0 | Tier III |
106 | NC_000001.11 | 1 | 120940033 | + | LINC00623 | NNNAAGAAGGAAGGTGGAGG | NGG | 2 | 0.0 | Tier III |
107 | NC_000001.11 | 1 | 145189018 | - | LINC01145 | NNNAAGAAGGAAGGTGGAGG | NGG | 2 | 0.0 | Tier III |
108 | NC_000001.11 | 1 | 149634192 | + | LINC00869 | NNNAAGAAGGAAGGTGGAGG | NGG | 2 | 0.0 | Tier III |
109 | NC_000007.14 | 7 | 97526217 | + | LOC105375416 | NNNAAGAAGGAATGTGGAGG | NAG | 2 | 0.0 | Tier III |
110 | NC_000018.10 | 18 | 73262946 | - | LINC02864 | NNNAAGAAGGAATTTGGAGG | NAC | 2 | 0.0 | Tier III |
111 | NC_000008.11 | 8 | 82493452 | + | LOC105375931 | NNNGAGAAGGAATTTTGAGG | NGG | 2 | 0.0 | Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
Reference Sequence | Chromosome | Cut Position | Match Strand | Match Gene | Match Sequence | PAM Seq. | Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. | CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. | Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. | |
---|---|---|---|---|---|---|---|---|---|---|
1 | NC_000075.6 | 9 | 106212312 | + | Twf2 | NNNAAGAAGGAGTTTGGAGG | NGG | 1 | 0.7222 | Tier I |
2 | NC_000081.6 | 15 | 94585488 | - | Twf1 | NNNAAGAAAGAATTTGGAGG | NGG | 1 | 0.6429 | Tier I |
3 | NC_000068.7 | 2 | 91931098 | - | Mdk | NNNAAGAAGGAATTTGGAGG | NGA | 1 | 0.0694 | Tier I |
4 | NC_000075.6 | 9 | 34873125 | - | Kirrel3 | NNNAAGAAGGAATTTGGAGA | NGG | 1 | 0.9375 | Tier II |
5 | NC_000073.6 | 7 | 97487729 | + | Ints4 | NNNAAGAAGGAATTTGAAGA | NGG | 2 | 0.875 | Tier II |
6 | NC_000073.6 | 7 | 44393063 | + | Syt3 | NNNAAAAAAGAATTTGGAGG | NGG | 2 | 0.6429 | Tier II |
7 | NC_000067.6 | 1 | 136006467 | - | Igfn1 | NNNAAGAAGGAATATGGAGG | NGG | 1 | 0.619 | Tier II |
8 | NC_000068.7 | 2 | 140886931 | + | Macrod2 | NNNAAGAAAGAATTTGGAGA | NGG | 2 | 0.6027 | Tier II |
9 | NC_000067.6 | 1 | 127451031 | - | Mgat5 | NNNAAGAAGGAATTAGAAGG | NGG | 2 | 0.5404 | Tier II |
10 | NC_000072.6 | 6 | 64420243 | + | Grid2 | NNNAAGAATGAATTTAGAGG | NGG | 2 | 0.5333 | Tier II |
11 | NC_000075.6 | 9 | 34937208 | - | Kirrel3 | NNNAGGAAGGAGTTTGGAGG | NGG | 2 | 0.52 | Tier II |
12 | NC_000070.6 | 4 | 151808267 | - | Camta1 | NNNAAGAAGGAATTTGGATT | NGG | 2 | 0.4667 | Tier II |
13 | NC_000083.6 | 17 | 81700643 | - | Slc8a1 | NNNGAGAAGGAATTTGGAAG | NGG | 2 | 0.4464 | Tier II |
14 | NC_000067.6 | 1 | 116207800 | + | Cntnap5a | NNNAAAAAGGAATTTGGAGC | NGG | 2 | 0.4286 | Tier II |
15 | NC_000072.6 | 6 | 37251767 | - | Dgki | NNNGAGAAGGAATTTGGATG | NGG | 2 | 0.4167 | Tier II |
16 | NC_000082.6 | 16 | 44272631 | + | Sidt1 | NNNAAGAAGAAATTTGGGGG | NGG | 2 | 0.3733 | Tier II |
17 | NC_000069.6 | 3 | 138189575 | - | 4930579F01Rik | NNNAAGCAGGAACTTGGAGG | NGG | 2 | 0.3454 | Tier II |
18 | NC_000077.6 | 11 | 36894773 | + | Tenm2 | NNNAAGTAGGAACTTGGAGG | NGG | 2 | 0.3454 | Tier II |
19 | NC_000080.6 | 14 | 93401299 | + | Pcdh9 | NNNATGAAGAAATTTGGAGG | NGG | 2 | 0.3394 | Tier II |
20 | NC_000086.7 | X | 134096358 | - | Nox1 | NNNAAAAAGGATTTTGGAGG | NGG | 2 | 0.3333 | Tier II |
21 | NC_000074.6 | 8 | 16992060 | + | Csmd1 | NNNAAGAAGTAATTTGGGGG | NGG | 2 | 0.325 | Tier II |
22 | NC_000073.6 | 7 | 3652697 | + | Cnot3 | NNNAAGGAGGAATTTGGAGC | NGG | 2 | 0.3025 | Tier II |
23 | NC_000067.6 | 1 | 34041307 | + | Dst | NNNAAGAAGGAATTTGGGGT | NGG | 2 | 0.28 | Tier II |
24 | NC_000074.6 | 8 | 17049404 | + | Csmd1 | NNNAAGAAGGAATTTGGAGG | NAG | 1 | 0.2593 | Tier II |
25 | NC_000073.6 | 7 | 112043453 | - | Usp47 | NNNTAGAAGCAATTTGGAGG | NGG | 2 | 0.2545 | Tier II |
26 | NC_000077.6 | 11 | 33860757 | + | Kcnip1 | NNNAAGAAGGAATTTGGAGA | NAG | 2 | 0.2431 | Tier II |
27 | NC_000068.7 | 2 | 120217338 | + | Pla2g4e | NNNCAGAAGGGATTTGGAGG | NGG | 2 | 0.2294 | Tier II |
28 | NC_000080.6 | 14 | 66914532 | - | Pnma2 | NNNGAGAAGGATTTTGGAGG | NGG | 2 | 0.2083 | Tier II |
29 | NC_000071.6 | 5 | 51502821 | + | Ppargc1a | NNNAAGAAGGAAACTGGAGG | NGG | 2 | 0.1978 | Tier II |
30 | NC_000067.6 | 1 | 11149710 | - | Prex2 | NNNAGGAAGGAATTTGGAGG | NAG | 2 | 0.1867 | Tier II |
31 | NC_000067.6 | 1 | 135673690 | + | Nav1 | NNNAAGAAGGACTTTGGATG | NGG | 2 | 0.1754 | Tier II |
32 | NC_000082.6 | 16 | 36611936 | - | Cd86 | NNNAAGAAAGAAGTTGGAGG | NGG | 2 | 0.1677 | Tier II |
33 | NC_000080.6 | 14 | 93055667 | - | Pcdh9 | NNNAAGAAGGAAGATGGAGG | NGG | 2 | 0.1615 | Tier II |
34 | NC_000077.6 | 11 | 80812773 | + | Tmem98 | NNNCAGAAGGAATTTGGAGC | NGG | 2 | 0.1513 | Tier II |
35 | NC_000073.6 | 7 | 144079297 | - | Shank2 | NNNAAGAAGGAAGTAGGAGG | NGG | 2 | 0.151 | Tier II |
36 | NC_000071.6 | 5 | 144211167 | - | Tecpr1 | NNNCAGAAGCAATTTGGAGG | NGG | 2 | 0.1412 | Tier II |
37 | NC_000075.6 | 9 | 16275292 | + | Fat3 | NNNAAGAATGACTTTGGAGG | NGG | 2 | 0.1404 | Tier II |
38 | NC_000077.6 | 11 | 59270441 | + | Wnt3a | NNNAAGAATGAAGTTGGAGG | NGG | 2 | 0.1391 | Tier II |
39 | NC_000072.6 | 6 | 99211906 | + | Foxp1 | NNNAAGAAGCATTTTGGAGG | NGG | 2 | 0.1333 | Tier II |
40 | NC_000070.6 | 4 | 35827003 | - | Lingo2 | NNNAAGATGGAATCTGGAGG | NGG | 2 | 0.1224 | Tier II |
41 | NC_000074.6 | 8 | 38155702 | + | Sgcz | NNNAAGTAGGAATTTGGAGG | NAG | 2 | 0.1134 | Tier II |
42 | NC_000067.6 | 1 | 70439058 | - | Spag16 | NNNAAGAAGGAATCTGGAGG | NAG | 2 | 0.0741 | Tier II |
43 | NC_000072.6 | 6 | 87086682 | - | Gfpt1 | NNNAAGAAGGAATTTGGAGA | NGA | 2 | 0.0651 | Tier II |
44 | NC_000073.6 | 7 | 100915048 | - | Arhgef17 | NNNAAGAAGGACTTTGCAGG | NGG | 2 | 0.0619 | Tier II |
45 | NC_000075.6 | 9 | 40208573 | + | Zfp202 | NNNAAGAAGAAATTGGGAGG | NGG | 2 | 0.0467 | Tier II |
46 | NC_000074.6 | 8 | 3411997 | - | Arhgef18 | NNNAAGAAGGAATTGGAAGG | NGG | 2 | 0.0467 | Tier II |
47 | NC_000072.6 | 6 | 38626962 | + | Klrg2 | NNNAAGAAGGAAATGGGAGG | NGG | 2 | 0.0346 | Tier II |
48 | NC_000073.6 | 7 | 102095526 | - | Trpc2 | NNNAAGAAGGAAATGGGAGG | NGG | 2 | 0.0346 | Tier II |
49 | NC_000073.6 | 7 | 102095526 | - | Xntrpc | NNNAAGAAGGAAATGGGAGG | NGG | 2 | 0.0346 | Tier II |
50 | NC_000085.6 | 19 | 32163725 | - | Sgms1 | NNNAGGAAGGAATTTGGAGG | NTG | 2 | 0.0281 | Tier II |
51 | NC_000073.6 | 7 | 25003238 | + | Atp1a3 | NNNAAGTAGGAATTGGGAGG | NGG | 2 | 0.0219 | Tier II |
52 | NC_000084.6 | 18 | 36375601 | - | Cystm1 | NNNAAGAAGCAATTGGGAGG | NGG | 2 | 0.02 | Tier II |
53 | NC_000078.6 | 12 | 72882982 | + | 4930447C04Rik | NNNAAGATGGAATTTGGAGG | NTG | 2 | 0.0167 | Tier II |
54 | NC_000067.6 | 1 | 152466653 | - | Colgalt2 | NNNAAGAAGGAATTGGGAGG | NAG | 2 | 0.013 | Tier II |
55 | NC_000085.6 | 19 | 26725971 | - | Smarca2 | NNNAAGAATGAATTTGGAGG | NGC | 2 | 0.0119 | Tier II |
56 | NC_000071.6 | 5 | 140960605 | - | Card11 | NNNGAGAAGGAATTTGGAGG | NGT | 2 | 0.0101 | Tier II |
57 | NC_000071.6 | 5 | 96651903 | - | Fras1 | NNNAAGAAGGATTTTGGAGG | NGC | 2 | 0.0074 | Tier II |
58 | NC_000075.6 | 9 | 16309744 | - | Fat3 | NNNAAGTAGGAATTTGGAGG | NGT | 2 | 0.0071 | Tier II |
59 | NC_000068.7 | 2 | 60480049 | + | Pla2r1 | NNNAAGAAGGAAAGTGGAGG | NGG | 2 | 0.0 | Tier II |
60 | NC_000081.6 | 15 | 80997319 | + | Sgsm3 | NNNAAGAAGGAAATTTGAGG | NGG | 2 | 0.0 | Tier II |
61 | NC_000086.7 | X | 38791716 | + | 6030498E09Rik | NNNAAGAAGGAAATTTGAGG | NGG | 2 | 0.0 | Tier II |
62 | NC_000070.6 | 4 | 138023235 | - | Eif4g3 | NNNAAGAAGGAAGGTGGAGG | NGG | 2 | 0.0 | Tier II |
63 | NC_000080.6 | 14 | 29028730 | + | Cacna2d3 | NNNAAGAAGGAAGTTTGAGG | NGG | 2 | 0.0 | Tier II |
64 | NC_000080.6 | 14 | 29028730 | + | Lrtm1 | NNNAAGAAGGAAGTTTGAGG | NGG | 2 | 0.0 | Tier II |
65 | NC_000078.6 | 12 | 104215798 | - | Serpina3f | NNNAAGAAGGAATGTGGAAG | NGG | 2 | 0.0 | Tier II |
66 | NC_000081.6 | 15 | 33685639 | - | Tspyl5 | NNNAAGAAGGAATTTTGAGG | NAG | 2 | 0.0 | Tier II |
67 | NC_000070.6 | 4 | 119973529 | - | Hivep3 | NNNAGGAAGGAATTTTGAGG | NGG | 2 | 0.0 | Tier II |
68 | NC_000071.6 | 5 | 66173708 | - | 1700126H18Rik | NNNAAGAAGAAACTTGGAGG | NGG | 2 | 0.7368 | Tier III |
69 | NC_000071.6 | 5 | 66173708 | - | Gm26725 | NNNAAGAAGAAACTTGGAGG | NGG | 2 | 0.7368 | Tier III |
70 | NC_000073.6 | 7 | 104821542 | + | Gm4887 | NNNAAGAAAGAATTTGGAGG | NGG | 1 | 0.6429 | Tier III |
71 | NC_000075.6 | 9 | 34937208 | - | Kirrel3os | NNNAGGAAGGAGTTTGGAGG | NGG | 2 | 0.52 | Tier III |
72 | NC_000078.6 | 12 | 104194014 | + | Serpina3e-ps | NNNAGGAAGGAATTTGGAAG | NGG | 2 | 0.5143 | Tier III |
73 | NC_000069.6 | 3 | 73518366 | + | Gm20754 | NNNAAGAAGGAATTTGGATT | NGG | 2 | 0.4667 | Tier III |
74 | NC_000070.6 | 4 | 29089566 | + | Gm52716 | NNNTGGAAGGAATTTGGAGG | NGG | 2 | 0.4582 | Tier III |
75 | NC_000071.6 | 5 | 127070461 | - | 4933438B17Rik | NNNAAGAAGGAAAATGGAGG | NGG | 2 | 0.4286 | Tier III |
76 | NC_000071.6 | 5 | 127070461 | - | Gm33491 | NNNAAGAAGGAAAATGGAGG | NGG | 2 | 0.4286 | Tier III |
77 | NC_000084.6 | 18 | 32586053 | + | Gm35480 | NNNGAGAAGGAATTTGGATG | NGG | 2 | 0.4167 | Tier III |
78 | NC_000067.6 | 1 | 179872757 | - | Gm31668 | NNNAAGAAGGAATAAGGAGG | NGG | 2 | 0.3584 | Tier III |
79 | NC_000069.6 | 3 | 126483705 | - | Gm46823 | NNNAAGGAGGAATTTGGTGG | NGG | 2 | 0.3529 | Tier III |
80 | NC_000083.6 | 17 | 4952564 | + | Gm41517 | NNNAAGCAGGAACTTGGAGG | NGG | 2 | 0.3454 | Tier III |
81 | NC_000081.6 | 15 | 60967259 | - | 4930402D18Rik | NNNAAGAAGGAATCTAGAGG | NGG | 2 | 0.2857 | Tier III |
82 | NC_000068.7 | 2 | 128365346 | + | Morrbid | NNNAAGATGGGATTTGGAGG | NGG | 2 | 0.2786 | Tier III |
83 | NC_000078.6 | 12 | 8051945 | + | Gm33037 | NNNAAGAAAGAATTTGGAGC | NGG | 2 | 0.2755 | Tier III |
84 | NC_000082.6 | 16 | 84056014 | - | Gm41481 | NNNAAGAAAGAATTTGGAGG | NAG | 2 | 0.1667 | Tier III |
85 | NC_000082.6 | 16 | 14989712 | - | Gm46561 | NNNAAGAAGGAAGATGGAGG | NGG | 2 | 0.1615 | Tier III |
86 | NC_000077.6 | 11 | 80812773 | + | Gm31228 | NNNCAGAAGGAATTTGGAGC | NGG | 2 | 0.1513 | Tier III |
87 | NC_000072.6 | 6 | 99211906 | + | Gm52870 | NNNAAGAAGCATTTTGGAGG | NGG | 2 | 0.1333 | Tier III |
88 | NC_000084.6 | 18 | 76625436 | + | Gm31933 | NNNAAGAAGGAATTTGGAGG | NTG | 1 | 0.039 | Tier III |
89 | NC_000083.6 | 17 | 49255015 | + | Gm30532 | NNNAAGAAGGAATTTTGAGG | NGT | 2 | 0.0 | Tier III |