Construct: sgRNA BRDN0001147791
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGGGTTGTACAAGGCCCTAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TAB1 (10454)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77792
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000022.11 |
22 |
39421937 |
+ |
TAB1 |
NNNGTTGTACAAGGCCCTAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
7626533 |
- |
SAT2 |
NNNCTTGTACAAGGCCCTAG |
NAG |
2 |
0.1373 |
Tier I |
| 3 |
NC_000016.10 |
16 |
89383903 |
- |
ANKRD11 |
NNNGTTTGACAAGGCCCTAG |
NGG |
2 |
0.419 |
Tier II |
| 4 |
NC_000016.10 |
16 |
89383903 |
- |
LOC128462377 |
NNNGTTTGACAAGGCCCTAG |
NGG |
2 |
0.419 |
Tier II |
| 5 |
NC_000019.10 |
19 |
50385046 |
- |
POLD1 |
NNNGTTTTACAAGGCCCTAT |
NGG |
2 |
0.4 |
Tier II |
| 6 |
NC_000022.11 |
22 |
19138062 |
- |
ESS2 |
NNNTCTGTACAAGGCCCTAG |
NGG |
2 |
0.3636 |
Tier II |
| 7 |
NC_000023.11 |
X |
50639760 |
+ |
SHROOM4 |
NNNGTAGTACAAGGCTCTAG |
NGG |
2 |
0.2667 |
Tier II |
| 8 |
NC_000001.11 |
1 |
240308314 |
- |
FMN2 |
NNNGTTCTACAAGTCCCTAG |
NGG |
2 |
0.1833 |
Tier II |
| 9 |
NC_000017.11 |
17 |
7626533 |
- |
SHBG |
NNNCTTGTACAAGGCCCTAG |
NAG |
2 |
0.1373 |
Tier II |
| 10 |
NC_000003.12 |
3 |
139579256 |
- |
NMNAT3 |
NNNGTTGTAGAAGGCTCTAG |
NGG |
2 |
0.1197 |
Tier II |
| 11 |
NC_000006.12 |
6 |
106056906 |
+ |
PRDM1 |
NNNGTTGTACAAGGCCCTGG |
NAG |
2 |
0.0972 |
Tier II |
| 12 |
NC_000011.10 |
11 |
12171285 |
+ |
MICAL2 |
NNNGTTGTACAAGGCCCTAG |
NGA |
1 |
0.0694 |
Tier II |
| 13 |
NC_000007.14 |
7 |
120610626 |
+ |
KCND2 |
NNNCTTGTACAAGGTCCTAG |
NGG |
2 |
0.0353 |
Tier II |
| 14 |
NC_000007.14 |
7 |
42089815 |
+ |
GLI3 |
NNNGTTGTACAAGGCCCCAG |
NTG |
2 |
0.0167 |
Tier II |
| 15 |
NC_000005.10 |
5 |
151420235 |
+ |
SLC36A1 |
NNNGTTATACAAGGCCCTAG |
NGT |
2 |
0.0161 |
Tier II |
| 16 |
NC_000003.12 |
3 |
139579256 |
- |
COPB2-DT |
NNNGTTGTAGAAGGCTCTAG |
NGG |
2 |
0.1197 |
Tier III |
| 17 |
NC_000005.10 |
5 |
151420235 |
+ |
LOC105378234 |
NNNGTTATACAAGGCCCTAG |
NGT |
2 |
0.0161 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
80155867 |
+ |
Tab1 |
NNNGCTGTACAAGGCCCTGG |
NGG |
2 |
0.375 |
Tier I |
| 2 |
NC_000079.6 |
13 |
70770897 |
+ |
Adamts16 |
NNNGCTGTACAAGGCCCTGG |
NGG |
2 |
0.375 |
Tier II |
| 3 |
NC_000083.6 |
17 |
11590965 |
- |
Prkn |
NNNGTTGCACAAGGCCCTAG |
NAG |
2 |
0.2593 |
Tier II |
| 4 |
NC_000075.6 |
9 |
45747288 |
- |
Dscaml1 |
NNNGTTGAACAAGGCCCTAG |
NGA |
2 |
0.0556 |
Tier II |
| 5 |
NC_000070.6 |
4 |
152534930 |
+ |
Nphp4 |
NNNGTTGGACAAGGCCCTAG |
NGA |
2 |
0.0509 |
Tier II |
| 6 |
NC_000068.7 |
2 |
146224866 |
- |
Insm1 |
NNNGTTGTACAAGGGCCTTG |
NGG |
2 |
0.0269 |
Tier II |
| 7 |
NC_000085.6 |
19 |
55642745 |
+ |
4930552P12Rik |
NNNGTTGTACAAGGCCCTGG |
NAG |
2 |
0.0972 |
Tier III |
| 8 |
NC_000082.6 |
16 |
26316473 |
- |
Gm41436 |
NNNGTTGTAGAAGGCCCTCG |
NGG |
2 |
0.0805 |
Tier III |
Other clones with same target sequence:
(none)
