Construct: sgRNA BRDN0001147793
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GATTTACTGGAGTTGCAAGT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- TLK1 (9874)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 75996
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
171117784 |
- |
TLK1 |
NNNTTACTGGAGTTGCAAGT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000010.11 |
10 |
110602660 |
+ |
SMC3 |
NNNTTACTGGAGTTGGAATT |
NGG |
2 |
0.1026 |
Tier I |
| 3 |
NC_000005.10 |
5 |
153524812 |
- |
GRIA1 |
NNNTCACTGGAGTTGCAATT |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000014.9 |
14 |
69929952 |
+ |
SMOC1 |
NNNTTTCTGGAGATGCAAGT |
NGG |
2 |
0.4945 |
Tier II |
| 5 |
NC_000007.14 |
7 |
93313872 |
+ |
VPS50 |
NNNTTACTGGACTTGCAATT |
NGG |
2 |
0.3529 |
Tier II |
| 6 |
NC_000004.12 |
4 |
37597121 |
- |
RELL1 |
NNNTGACTGGATTTGCAAGT |
NGG |
2 |
0.2462 |
Tier II |
| 7 |
NC_000002.12 |
2 |
240496226 |
- |
ANKMY1 |
NNNTTAATGGAGTTTCAAGT |
NGG |
2 |
0.1071 |
Tier II |
| 8 |
NC_000002.12 |
2 |
99102390 |
+ |
TSGA10 |
NNNTTACTGGAGTTGGAATT |
NGG |
2 |
0.1026 |
Tier II |
| 9 |
NC_000008.11 |
8 |
28825842 |
+ |
INTS9 |
NNNTTACTGGTGTTTCAAGT |
NGG |
2 |
0.044 |
Tier II |
| 10 |
NC_000018.10 |
18 |
76450269 |
- |
ZNF516 |
NNNTTACTGGAGTGGCAAGT |
NGG |
1 |
0.0 |
Tier II |
| 11 |
NC_000006.12 |
6 |
125379657 |
+ |
LOC102723341 |
NNNTTACAGGAGTTGAAAGT |
NGG |
2 |
0.8 |
Tier III |
| 12 |
NC_000014.9 |
14 |
22017657 |
- |
TRA |
NNNTTACTGGAGCTGCAAAT |
NGG |
2 |
0.5639 |
Tier III |
| 13 |
NC_000009.12 |
9 |
122686256 |
+ |
TLK1P1 |
NNNTTATTGGAGTTGTAAGT |
NGG |
2 |
0.25 |
Tier III |
| 14 |
NC_000002.12 |
2 |
99102390 |
+ |
SMC3P1 |
NNNTTACTGGAGTTGGAATT |
NGG |
2 |
0.1026 |
Tier III |
| 15 |
NC_000003.12 |
3 |
166143441 |
- |
LOC124909497 |
NNNTTACTGGAGTTGAAAGT |
NTG |
2 |
0.039 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
70786922 |
- |
Tlk1 |
NNNTTACTGGAGTTGCTACT |
NGG |
2 |
0.0598 |
Tier I |
| 2 |
NC_000071.6 |
5 |
16178659 |
+ |
Cacna2d1 |
NNNTTACTGGAGTTGAAATT |
NGG |
2 |
0.6667 |
Tier II |
| 3 |
NC_000070.6 |
4 |
46680799 |
- |
Gabbr2 |
NNNGCACTGGAGTTGCAAGT |
NGG |
2 |
0.625 |
Tier II |
| 4 |
NC_000071.6 |
5 |
150727340 |
- |
Pds5b |
NNNTTACTGAAGTTGCAAGT |
NAG |
2 |
0.242 |
Tier II |
| 5 |
NC_000080.6 |
14 |
77452080 |
+ |
Enox1 |
NNNTTGCTGGAGTTGCAAGT |
NAG |
2 |
0.1852 |
Tier II |
| 6 |
NC_000073.6 |
7 |
64519361 |
- |
Apba2 |
NNNTTACTGGTGTTGCAGGT |
NGG |
2 |
0.1231 |
Tier II |
| 7 |
NC_000068.7 |
2 |
17638909 |
+ |
Nebl |
NNNTTACTGGAGTTGGAATT |
NGG |
2 |
0.1026 |
Tier II |
| 8 |
NC_000070.6 |
4 |
62383785 |
+ |
Slc31a1 |
NNNTTACTGGAGTTGAAAGT |
NGA |
2 |
0.0694 |
Tier II |
| 9 |
NC_000083.6 |
17 |
80435243 |
+ |
Sos1 |
NNNGTACTGGAGTTGCAAGT |
NGC |
2 |
0.0139 |
Tier II |
| 10 |
NC_000071.6 |
5 |
81738484 |
- |
Adgrl3 |
NNNCTACTGGAGTTGCAAGT |
NGT |
2 |
0.0104 |
Tier II |
Other clones with same target sequence:
(none)
