Construct: sgRNA BRDN0001147795
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CCCCGACCATAATGGTACAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MINK1 (50488)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76262
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000017.11 |
17 |
4890682 |
- |
MINK1 |
NNNCGACCATAATGGTACAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
28044745 |
- |
OCA2 |
NNNAGACCATAATGATACAG |
NGG |
2 |
0.7926 |
Tier II |
| 3 |
NC_000023.11 |
X |
14454656 |
- |
GLRA2 |
NNNCAACCAAAATGGTACAG |
NGG |
2 |
0.7429 |
Tier II |
| 4 |
NC_000002.12 |
2 |
9902161 |
- |
TAF1B |
NNNAGACCTTAATGGTACAG |
NGG |
2 |
0.5053 |
Tier II |
| 5 |
NC_000012.12 |
12 |
46778072 |
- |
SLC38A4 |
NNNCCACCATAATGGTTCAG |
NGG |
2 |
0.1048 |
Tier II |
| 6 |
NC_000023.11 |
X |
40113628 |
+ |
BCOR |
NNNCGTCCATAATGGTACAG |
NGT |
2 |
0.0115 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000077.6 |
11 |
70607091 |
- |
Mink1 |
NNNCGACCGTAATGATACAG |
NGG |
2 |
0.6275 |
Tier I |
| 2 |
NC_000071.6 |
5 |
81695020 |
+ |
Adgrl3 |
NNNAGACTATAATGGTACAG |
NGG |
2 |
0.7368 |
Tier II |
| 3 |
NC_000075.6 |
9 |
92283926 |
- |
Plscr2 |
NNNCAACCATAATAGTACAG |
NGG |
2 |
0.65 |
Tier II |
| 4 |
NC_000070.6 |
4 |
48160458 |
+ |
Stx17 |
NNNCTACCATATTGGTACAG |
NGG |
2 |
0.1 |
Tier II |
Other clones with same target sequence:
(none)
