Construct: sgRNA BRDN0001147797
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTCCTCATCCGGTCAGGCTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ONE_INTERGENIC_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80204
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
46361770 |
+ |
NSUN4 |
NNNCTCATCCTGTCAGGTTG |
NGG |
2 |
0.2473 |
Tier I |
| 2 |
NC_000001.11 |
1 |
3225505 |
- |
PRDM16 |
NNNATCATCAGGTCAGGCTG |
NGG |
2 |
0.7298 |
Tier II |
| 3 |
NC_000002.12 |
2 |
88179251 |
- |
THNSL2 |
NNNCACATCAGGTCAGGCTG |
NGG |
2 |
0.4333 |
Tier II |
| 4 |
NC_000008.11 |
8 |
129405749 |
- |
CCDC26 |
NNNCCCATCAGGTCAGGCTG |
NGG |
2 |
0.8667 |
Tier III |
| 5 |
NC_000017.11 |
17 |
30238639 |
- |
LOC105371720 |
NNNCTCATGCGGTCAGGCTC |
NGG |
2 |
0.2653 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
74354168 |
- |
Slco3a1 |
NNNCTCATCTGGTCAGGCTT |
NGG |
2 |
0.6588 |
Tier II |
| 2 |
NC_000073.6 |
7 |
141845703 |
+ |
Muc5b |
NNNCTCATCAGTTCAGGCTG |
NGG |
2 |
0.3333 |
Tier II |
| 3 |
NC_000071.6 |
5 |
122676131 |
- |
P2rx7 |
NNNCTCATCCAGGCAGGCTG |
NGG |
2 |
0.2609 |
Tier II |
| 4 |
NC_000077.6 |
11 |
62879892 |
- |
Tvp23b |
NNNCTCATCCGGGCAGGCTA |
NGG |
2 |
0.2446 |
Tier II |
| 5 |
NC_000078.6 |
12 |
8340995 |
+ |
Hs1bp3 |
NNNCTCATCCCGTCAGCCTG |
NGG |
2 |
0.1008 |
Tier II |
| 6 |
NC_000076.6 |
10 |
85651344 |
- |
Btbd11 |
NNNCTCATCCAGTCAGGCTG |
NGC |
2 |
0.0222 |
Tier II |
| 7 |
NC_000068.7 |
2 |
172665256 |
- |
Gm36156 |
NNNCTCATCCACTCAGGCTG |
NGG |
2 |
0.5294 |
Tier III |
| 8 |
NC_000079.6 |
13 |
37620101 |
- |
Gm40915 |
NNNGTCATCCGGCCAGGCTG |
NGG |
2 |
0.3947 |
Tier III |
| 9 |
NC_000068.7 |
2 |
63542965 |
- |
Gm8541 |
NNNCTCATCCTCTCAGGCTG |
NGG |
2 |
0.2036 |
Tier III |
Other clones with same target sequence:
(none)
