Construct: sgRNA BRDN0001147800
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTTTAGGGAGGTACGACTT
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- ROCK2 (9475)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76349
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000002.12 |
2 |
11222165 |
- |
ROCK2 |
NNNTTAGGGAGGTACGACTT |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
69314389 |
- |
ABCA5 |
NNNTTAGGGAGGTAGGAGTT |
NGG |
2 |
0.0067 |
Tier I |
| 3 |
NC_000007.14 |
7 |
22204678 |
+ |
RAPGEF5 |
NNNTTAGGGAGGCACGCCTT |
NGG |
2 |
0.1393 |
Tier II |
| 4 |
NC_000005.10 |
5 |
139966403 |
+ |
NRG2 |
NNNTTACGGAGGTATGACTT |
NGG |
2 |
0.0458 |
Tier II |
| 5 |
NC_000008.11 |
8 |
97912514 |
+ |
MATN2 |
NNNTTAGGGAGTTACGACTT |
NTG |
2 |
0.015 |
Tier II |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
16952506 |
+ |
Rock2 |
NNNTTAGGGAGGTACGACTT |
NGA |
1 |
0.0694 |
Tier I |
| 2 |
NC_000074.6 |
8 |
79363813 |
- |
Smad1 |
NNNTTAGGCAGGTATGACTT |
NGG |
2 |
0.0359 |
Tier II |
| 3 |
NC_000073.6 |
7 |
139522742 |
+ |
Inpp5a |
NNNTTAGGGAGGTAAGACTT |
NTG |
2 |
0.0087 |
Tier II |
Other clones with same target sequence:
(none)
