Construct: sgRNA BRDN0001147805
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AAGCACCAGATCATGCACCG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP2K2 (5605)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77600
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000019.10 |
19 |
4101234 |
- |
MAP2K2 |
NNNCACCAGATCATGCACCG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000015.10 |
15 |
66444703 |
+ |
MAP2K1 |
NNNCACAAGATCATGCACAG |
NGG |
2 |
0.3214 |
Tier I |
| 3 |
NC_000015.10 |
15 |
63922767 |
- |
DAPK2 |
NNNCAGCAGATCATGCACTG |
NGG |
2 |
0.2308 |
Tier I |
| 4 |
NC_000007.14 |
7 |
149224335 |
+ |
ZNF282 |
NNNCACCAGATGACGCACCG |
NGG |
2 |
0.127 |
Tier I |
| 5 |
NC_000019.10 |
19 |
46387374 |
+ |
PPP5C |
NNNCCCCAGATCATGCACGG |
NGG |
2 |
0.0625 |
Tier I |
| 6 |
NC_000019.10 |
19 |
14479423 |
+ |
GIPC1 |
NNNCACCAGATCCTCCACCG |
NGG |
2 |
0.0574 |
Tier I |
| 7 |
NC_000008.11 |
8 |
98470333 |
- |
STK3 |
NNNCACAAGATCATGCACTG |
NGG |
2 |
0.3462 |
Tier II |
| 8 |
NC_000001.11 |
1 |
3161810 |
- |
PRDM16 |
NNNCACCAGATCCTGCACCC |
NGG |
2 |
0.0902 |
Tier II |
| 9 |
NC_000009.12 |
9 |
122459610 |
+ |
OR1J2 |
NNNCACCAGTTCATGCACCG |
NTG |
2 |
0.0344 |
Tier II |
| 10 |
NC_000020.11 |
20 |
15848308 |
- |
MACROD2 |
NNNCACCAGATCCTGCACGG |
NGG |
2 |
0.0263 |
Tier II |
| 11 |
NC_000001.11 |
1 |
3161810 |
- |
LOC105378606 |
NNNCACCAGATCCTGCACCC |
NGG |
2 |
0.0902 |
Tier III |
| 12 |
NC_000019.10 |
19 |
46387374 |
+ |
LOC105372426 |
NNNCCCCAGATCATGCACGG |
NGG |
2 |
0.0625 |
Tier III |
| 13 |
NC_000007.14 |
7 |
129127090 |
+ |
LOC407835 |
NNNCACCAGATCATGCACCG |
NAA |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000076.6 |
10 |
81119156 |
+ |
Map2k2 |
NNNCACCAGATCATGCACAG |
NGG |
1 |
0.4286 |
Tier I |
| 2 |
NC_000072.6 |
6 |
47905072 |
+ |
Zfp282 |
NNNCACCAGATGACGCACCG |
NGG |
2 |
0.127 |
Tier I |
| 3 |
NC_000086.7 |
X |
73422311 |
+ |
Zfp92 |
NNNCACCAGCTCATCCACCG |
NGG |
2 |
0.0909 |
Tier I |
| 4 |
NC_000071.6 |
5 |
144002550 |
+ |
Ccz1 |
NNNCACTAGACCATGCACCG |
NGG |
2 |
0.5417 |
Tier II |
| 5 |
NC_000067.6 |
1 |
85671430 |
- |
Sp100 |
NNNCAACAGATCATGCACAG |
NGG |
2 |
0.398 |
Tier II |
| 6 |
NC_000073.6 |
7 |
50135063 |
- |
Nell1 |
NNNCACCAGCTCATGCACCA |
NGG |
2 |
0.3125 |
Tier II |
| 7 |
NC_000069.6 |
3 |
86129243 |
+ |
Sh3d19 |
NNNCACCAGGTCATCCACCG |
NGG |
2 |
0.1515 |
Tier II |
| 8 |
NC_000072.6 |
6 |
41085032 |
+ |
Tcrb |
NNNCTCCAGATCATGCACAG |
NGG |
2 |
0.1558 |
Tier III |
| 9 |
NC_000072.6 |
6 |
41085032 |
+ |
Trbv8 |
NNNCTCCAGATCATGCACAG |
NGG |
2 |
0.1558 |
Tier III |
Other clones with same target sequence:
(none)
