Construct: sgRNA BRDN0001147806
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGGTAAGGCATATCCAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDGFRB (5159)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77059
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000005.10 |
5 |
150133723 |
+ |
PDGFRB |
NNNGGTAAGGCATATCCAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
140551834 |
+ |
ELMOD2 |
NNNGGTAAGACATATCCAAT |
NGG |
2 |
0.6533 |
Tier II |
| 3 |
NC_000020.11 |
20 |
17238083 |
+ |
PCSK2 |
NNNGGTAAGGCCTATCCAAG |
NGG |
1 |
0.2632 |
Tier II |
| 4 |
NC_000005.10 |
5 |
145630397 |
- |
PRELID2 |
NNNGGTAAGGCATACCCAAG |
NGC |
2 |
0.0061 |
Tier II |
| 5 |
NC_000020.11 |
20 |
17238083 |
+ |
LOC105372546 |
NNNGGTAAGGCCTATCCAAG |
NGG |
1 |
0.2632 |
Tier III |
| 6 |
NC_000006.12 |
6 |
80610042 |
+ |
LOC112267962 |
NNNGGTAAGGCAAATGCAAG |
NGG |
2 |
0.1065 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000067.6 |
1 |
184983219 |
- |
Mark1 |
NNNGGTAAGGCATATCCAAC |
NGG |
1 |
0.4286 |
Tier II |
| 2 |
NC_000067.6 |
1 |
25328318 |
+ |
Adgrb3 |
NNNGGTTAGGCAGATCCAAG |
NGG |
2 |
0.1141 |
Tier II |
| 3 |
NC_000067.6 |
1 |
88376505 |
+ |
Trpm8 |
NNNGGTAAGGCAGATCCAGG |
NGG |
2 |
0.0978 |
Tier II |
| 4 |
NC_000079.6 |
13 |
29971080 |
+ |
E2f3 |
NNNGGTAATGCATATGCAAG |
NGG |
2 |
0.0821 |
Tier II |
| 5 |
NC_000079.6 |
13 |
48110187 |
- |
A330033J07Rik |
NNNGGTGAGGCATATCCAAA |
NGG |
2 |
0.6618 |
Tier III |
| 6 |
NC_000079.6 |
13 |
48110187 |
- |
Gm36346 |
NNNGGTGAGGCATATCCAAA |
NGG |
2 |
0.6618 |
Tier III |
| 7 |
NC_000081.6 |
15 |
62095906 |
+ |
Pvt1 |
NNNGGTATGTCATATCCAAG |
NGG |
2 |
0.3482 |
Tier III |
| 8 |
NC_000078.6 |
12 |
8134395 |
+ |
Gm33037 |
NNNGGCAAGGCATCTCCAAG |
NGG |
2 |
0.1948 |
Tier III |
Other clones with same target sequence:
(none)
