Construct: sgRNA BRDN0001147807
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGCAGACAAGGACCGTGACA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- FES (2242)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76509
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
90887199 |
+ |
FES |
NNNAGACAAGGACCGTGACA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000012.12 |
12 |
53288649 |
+ |
ESPL1 |
NNNAGACAAGGAGAGTGACA |
NGG |
2 |
0.0477 |
Tier I |
| 3 |
NC_000001.11 |
1 |
44235655 |
- |
ERI3 |
NNNAGACAAAGACCATGACA |
NGG |
2 |
0.8784 |
Tier II |
| 4 |
NC_000014.9 |
14 |
61044099 |
+ |
SLC38A6 |
NNNAGACAAGAACTGTGACA |
NGG |
2 |
0.7333 |
Tier II |
| 5 |
NC_000023.11 |
X |
3682042 |
+ |
PRKX |
NNNACACAGGGACCGTGACA |
NGG |
2 |
0.5238 |
Tier II |
| 6 |
NC_000007.14 |
7 |
90933728 |
+ |
CDK14 |
NNNAGACAAGGACAGAGACA |
NGG |
2 |
0.3182 |
Tier II |
| 7 |
NC_000017.11 |
17 |
76090899 |
+ |
EXOC7 |
NNNAGACAAGGACAGAGACA |
NGG |
2 |
0.3182 |
Tier II |
| 8 |
NC_000015.10 |
15 |
47692055 |
+ |
SEMA6D |
NNNAGACAAAGACCCTGACA |
NGG |
2 |
0.2545 |
Tier II |
| 9 |
NC_000006.12 |
6 |
11330642 |
+ |
NEDD9 |
NNNAGACAAGGTCCGTGACA |
NAG |
2 |
0.0864 |
Tier II |
| 10 |
NC_000018.10 |
18 |
53123805 |
- |
DCC |
NNNAGAAAAGGACCGTGACA |
NGT |
2 |
0.0121 |
Tier II |
| 11 |
NC_000001.11 |
1 |
44235655 |
- |
ERI3-IT1 |
NNNAGACAAAGACCATGACA |
NGG |
2 |
0.8784 |
Tier III |
| 12 |
NC_000014.9 |
14 |
61044099 |
+ |
LOC101927756 |
NNNAGACAAGAACTGTGACA |
NGG |
2 |
0.7333 |
Tier III |
| 13 |
NC_000024.10 |
Y |
7299119 |
- |
PRKY |
NNNACACAGGGACCGTGACA |
NGG |
2 |
0.5238 |
Tier III |
| 14 |
NC_000006.12 |
6 |
11330642 |
+ |
LOC105374925 |
NNNAGACAAGGTCCGTGACA |
NAG |
2 |
0.0864 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000073.6 |
7 |
80384006 |
- |
Fes |
NNNAGACAAGGATCGAGACA |
NGG |
2 |
0.6364 |
Tier I |
| 2 |
NC_000077.6 |
11 |
101738228 |
+ |
Dhx8 |
NNNGGACAGGGACCGTGACA |
NGG |
2 |
0.4167 |
Tier I |
| 3 |
NC_000083.6 |
17 |
8801429 |
- |
Pde10a |
NNNAGACAGGGACCGGGACA |
NGG |
2 |
0.2308 |
Tier I |
| 4 |
NC_000070.6 |
4 |
149923834 |
- |
Spsb1 |
NNNAGACAGGGACTGTGACA |
NGG |
2 |
0.4889 |
Tier II |
| 5 |
NC_000084.6 |
18 |
71777478 |
- |
Dcc |
NNNAGCCAAGGACCATGACA |
NGG |
2 |
0.4278 |
Tier II |
| 6 |
NC_000077.6 |
11 |
109986232 |
+ |
Abca8b |
NNNAGACCAGGACCATGACA |
NGG |
2 |
0.4034 |
Tier II |
| 7 |
NC_000071.6 |
5 |
114166885 |
- |
Acacb |
NNNAGACAAGGACAATGACA |
NGG |
2 |
0.3294 |
Tier II |
| 8 |
NC_000072.6 |
6 |
142662852 |
+ |
Abcc9 |
NNNAGACAAGGACAGAGACA |
NGG |
2 |
0.3182 |
Tier II |
| 9 |
NC_000072.6 |
6 |
53926942 |
+ |
Cpvl |
NNNAGAAAAGGACAGTGACA |
NGG |
2 |
0.2625 |
Tier II |
| 10 |
NC_000073.6 |
7 |
29409688 |
+ |
Sipa1l3 |
NNNAGGCAAGGACAGTGACA |
NGG |
2 |
0.25 |
Tier II |
| 11 |
NC_000068.7 |
2 |
158202452 |
- |
D630003M21Rik |
NNNAGACAAGGACCTTGAAA |
NGG |
2 |
0.0612 |
Tier II |
| 12 |
NC_000086.7 |
X |
16715468 |
+ |
Maob |
NNNAGATAAGGACCGTGACA |
NGA |
2 |
0.0564 |
Tier II |
| 13 |
NC_000082.6 |
16 |
89869576 |
- |
Tiam1 |
NNNAGACAAGGAACTTGACA |
NGG |
2 |
0.0549 |
Tier II |
| 14 |
NC_000074.6 |
8 |
61621116 |
+ |
Palld |
NNNAGACAAGGACAGTGACA |
NGA |
2 |
0.0243 |
Tier II |
| 15 |
NC_000067.6 |
1 |
38091318 |
+ |
Rev1 |
NNNAGACAAGGACAGTGACA |
NTG |
2 |
0.0136 |
Tier II |
| 16 |
NC_000071.6 |
5 |
37531514 |
- |
Stk32b |
NNNAGACAAGGACGGTGACA |
NGC |
2 |
0.0 |
Tier II |
| 17 |
NC_000079.6 |
13 |
43950432 |
- |
Gm2233 |
NNNAGACAAGGGCTGTGACA |
NGG |
2 |
0.5296 |
Tier III |
| 18 |
NC_000073.6 |
7 |
59315814 |
- |
Snhg14 |
NNNAGACAAGGCCAGTGACA |
NGG |
2 |
0.0921 |
Tier III |
| 19 |
NC_000074.6 |
8 |
61621116 |
+ |
Gm35254 |
NNNAGACAAGGACAGTGACA |
NGA |
2 |
0.0243 |
Tier III |
Other clones with same target sequence:
(none)
