Construct: sgRNA BRDN0001147809
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TGTGAAAACGAGCTCCACTG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIP4K2A (5305)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76574
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
22591732 |
+ |
PIP4K2A |
NNNGAAAACGAGCTCCACTG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000005.10 |
5 |
149924000 |
- |
PDE6A |
NNNGAAAAAGAGCTCAACTG |
NGG |
2 |
0.8571 |
Tier II |
| 3 |
NC_000013.11 |
13 |
98388893 |
- |
FARP1 |
NNNGAAAATGAGTTCCACTG |
NGG |
2 |
0.6125 |
Tier II |
| 4 |
NC_000007.14 |
7 |
50340157 |
+ |
IKZF1 |
NNNGAAAACTACCTCCACTG |
NGG |
2 |
0.4301 |
Tier II |
| 5 |
NC_000011.10 |
11 |
20756847 |
- |
NELL1 |
NNNGAATACGAGCTCCACTA |
NGG |
2 |
0.4102 |
Tier II |
| 6 |
NC_000003.12 |
3 |
189316463 |
+ |
TPRG1 |
NNNGAAAACCAGCTCAACTG |
NGG |
2 |
0.4 |
Tier II |
| 7 |
NC_000009.12 |
9 |
571171 |
+ |
KANK1 |
NNNGAAAACCAGCTCCACTG |
NGG |
1 |
0.4 |
Tier II |
| 8 |
NC_000018.10 |
18 |
8133337 |
- |
PTPRM |
NNNGAACATGAGCTCCACTG |
NGG |
2 |
0.3828 |
Tier II |
| 9 |
NC_000007.14 |
7 |
55562753 |
+ |
VOPP1 |
NNNGAAACTGAGCTCCACTG |
NGG |
2 |
0.375 |
Tier II |
| 10 |
NC_000019.10 |
19 |
11768918 |
+ |
ZNF441 |
NNNGAAAACCAACTCCACTG |
NGG |
2 |
0.3733 |
Tier II |
| 11 |
NC_000011.10 |
11 |
12828060 |
- |
TEAD1 |
NNNGACAACTAGCTCCACTG |
NGG |
2 |
0.3693 |
Tier II |
| 12 |
NC_000005.10 |
5 |
66610610 |
+ |
MAST4 |
NNNGAACACGAGTTCCACTG |
NGG |
2 |
0.3062 |
Tier II |
| 13 |
NC_000011.10 |
11 |
105862739 |
- |
GRIA4 |
NNNGAAAACCATCTCCACTG |
NGG |
2 |
0.1538 |
Tier II |
| 14 |
NC_000017.11 |
17 |
82108322 |
+ |
CCDC57 |
NNNGAAAACGGGCTCCTCTG |
NGG |
2 |
0.0867 |
Tier II |
| 15 |
NC_000011.10 |
11 |
66495348 |
- |
DPP3 |
NNNGAAAAGGAGCTCCACTG |
NCG |
2 |
0.0663 |
Tier II |
| 16 |
NC_000018.10 |
18 |
8133337 |
- |
LOC107985119 |
NNNGAACATGAGCTCCACTG |
NGG |
2 |
0.3828 |
Tier III |
| 17 |
NC_000011.10 |
11 |
12828060 |
- |
LOC107984313 |
NNNGACAACTAGCTCCACTG |
NGG |
2 |
0.3693 |
Tier III |
| 18 |
NC_000004.12 |
4 |
143158898 |
- |
USP38-DT |
NNNGAAAAAGAGCTCTACTG |
NGG |
2 |
0.2637 |
Tier III |
| 19 |
NC_000017.11 |
17 |
72090140 |
+ |
SOX9-AS1 |
NNNGAAAACGAGCTGCCCTG |
NGG |
2 |
0.0088 |
Tier III |
| 20 |
NC_000017.11 |
17 |
72090140 |
+ |
LINC02097 |
NNNGAAAACGAGCTGCCCTG |
NGG |
2 |
0.0088 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000081.6 |
15 |
102110706 |
- |
Tns2 |
NNNGAAAACAAACTCCACTG |
NGG |
2 |
0.8711 |
Tier I |
| 2 |
NC_000069.6 |
3 |
101384615 |
- |
Igsf3 |
NNNGAAAACAAGCTCCACTT |
NGG |
2 |
0.6533 |
Tier II |
| 3 |
NC_000073.6 |
7 |
138342983 |
+ |
Tcerg1l |
NNNGAAAACGAGCTCCACGG |
NAG |
2 |
0.0648 |
Tier II |
| 4 |
NC_000082.6 |
16 |
36060197 |
+ |
Fam162a |
NNNGAAAACGAGCATCACTG |
NGG |
2 |
0.0413 |
Tier II |
| 5 |
NC_000077.6 |
11 |
76511335 |
+ |
Abr |
NNNGAAAAGGAGCTCCACTG |
NGC |
2 |
0.0138 |
Tier II |
| 6 |
NC_000073.6 |
7 |
135173395 |
+ |
Dock1 |
NNNGAAAACGAGCTCCAGTG |
NGT |
2 |
0.0022 |
Tier II |
| 7 |
NC_000070.6 |
4 |
42170663 |
+ |
Gm52695 |
NNNGAAAACGGGTTCCACTG |
NGG |
2 |
0.455 |
Tier III |
| 8 |
NC_000070.6 |
4 |
42667822 |
+ |
Gm42276 |
NNNGAAAACGGGTTCCACTG |
NGG |
2 |
0.455 |
Tier III |
| 9 |
NT_187053.1 |
4 |
5020 |
- |
LOC105247290 |
NNNGAAAACGGGTTCCACTG |
NGG |
2 |
0.455 |
Tier III |
| 10 |
NT_187054.1 |
4 |
15631 |
+ |
LOC105247291 |
NNNGAAAACGGGTTCCACTG |
NGG |
2 |
0.455 |
Tier III |
| 11 |
NC_000068.7 |
2 |
160238901 |
+ |
Gm34805 |
NNNGAAAACATGCTCCACTG |
NGG |
2 |
0.2872 |
Tier III |
| 12 |
NC_000077.6 |
11 |
76511335 |
+ |
Gm12341 |
NNNGAAAAGGAGCTCCACTG |
NGC |
2 |
0.0138 |
Tier III |
Other clones with same target sequence:
(none)
