Construct: sgRNA BRDN0001147813
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GTGAAGGGGGTAATGTGACG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MTOR (2475)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76783
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
11248002 |
+ |
MTOR |
NNNAAGGGGGTAATGTGACG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
62777095 |
- |
MARCHF10 |
NNNAAGGGGATAATGTGACT |
NGG |
2 |
0.6533 |
Tier II |
| 3 |
NC_000012.12 |
12 |
128679859 |
+ |
TMEM132C |
NNNAAGAGGGTAATGGGACG |
NGG |
2 |
0.3462 |
Tier II |
| 4 |
NC_000002.12 |
2 |
158513664 |
- |
PKP4 |
NNNAAGGTGGTAATGTGACC |
NGG |
2 |
0.2679 |
Tier II |
| 5 |
NC_000015.10 |
15 |
93050603 |
- |
RGMA |
NNNATGGGGGTGATGTGACG |
NGG |
2 |
0.2626 |
Tier II |
| 6 |
NC_000001.11 |
1 |
162189755 |
+ |
NOS1AP |
NNNAAGAGGGTAATGTGACG |
NAG |
2 |
0.2593 |
Tier II |
| 7 |
NC_000017.11 |
17 |
68490830 |
- |
PRKAR1A |
NNNAAGCGGGTAATGTGACG |
NGA |
2 |
0.0477 |
Tier II |
| 8 |
NC_000019.10 |
19 |
31345614 |
- |
TSHZ3 |
NNNAAGGGGGTAATGTTCCG |
NGG |
2 |
0.0476 |
Tier II |
| 9 |
NC_000004.12 |
4 |
81461835 |
+ |
RASGEF1B |
NNNAAGGGGGTAAGGTGACC |
NGG |
2 |
0.0 |
Tier II |
| 10 |
NC_000014.9 |
14 |
100252608 |
- |
YY1 |
NNNAAGGGGGTAAGGTGATG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000004.12 |
4 |
144493045 |
- |
LOC105377462 |
NNNAAGGGGGTAATGTGAAC |
NGG |
2 |
0.1837 |
Tier III |
| 12 |
NC_000019.10 |
19 |
31345614 |
- |
TSHZ3-AS1 |
NNNAAGGGGGTAATGTTCCG |
NGG |
2 |
0.0476 |
Tier III |
| 13 |
NC_000010.11 |
10 |
6962834 |
- |
LOC105376387 |
NNNAAGGGGGTTATGTGAGG |
NGG |
2 |
0.0417 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
23348611 |
+ |
Kcnma1 |
NNNAAGAGGGTGATGTGACG |
NGG |
2 |
0.7222 |
Tier II |
| 2 |
NC_000072.6 |
6 |
83877405 |
- |
Zfp638 |
NNNAAGGGGGTAATGGGAGG |
NGG |
2 |
0.0433 |
Tier II |
| 3 |
NC_000077.6 |
11 |
55395532 |
+ |
Sparc |
NNNAAGGGGGTAATGGGAGG |
NGG |
2 |
0.0433 |
Tier II |
| 4 |
NC_000086.7 |
X |
138432023 |
+ |
Il1rapl2 |
NNNAAGGGGGTACTGTGAGG |
NGG |
2 |
0.0263 |
Tier II |
| 5 |
NC_000080.6 |
14 |
76677769 |
- |
1700108F19Rik |
NNNAAGGGGGAAATGTGACT |
NGG |
2 |
0.525 |
Tier III |
| 6 |
NC_000070.6 |
4 |
120239680 |
- |
Gm36134 |
NNNATGGGGGTAATGTGACC |
NGG |
2 |
0.1558 |
Tier III |
Other clones with same target sequence:
(none)
