Construct: sgRNA BRDN0001147815
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CTTGATTAGGAGGATTCATG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- LATS1 (9113)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76026
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000006.12 |
6 |
149684160 |
+ |
LATS1 |
NNNGATTAGGAGGATTCATG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
37697237 |
+ |
CDCA8 |
NNNCACTAGGAGGATTCATG |
NGG |
2 |
0.4813 |
Tier II |
| 3 |
NC_000003.12 |
3 |
53720020 |
- |
CACNA1D |
NNNGATAAGGAGGATTAATG |
NGG |
2 |
0.4083 |
Tier II |
| 4 |
NC_000014.9 |
14 |
94226333 |
- |
PPP4R4 |
NNNGATTAGCAAGATTCATG |
NGG |
2 |
0.3733 |
Tier II |
| 5 |
NC_000003.12 |
3 |
121438007 |
+ |
POLQ |
NNNGATTAGGAGGATGCATG |
NGG |
1 |
0.3462 |
Tier II |
| 6 |
NC_000001.11 |
1 |
156546632 |
- |
IQGAP3 |
NNNGATTAGGAGGATTCAGT |
NGG |
2 |
0.175 |
Tier II |
| 7 |
NC_000003.12 |
3 |
1123260 |
- |
CNTN6 |
NNNGATTAGGAGGCTTAATG |
NGG |
2 |
0.1 |
Tier II |
| 8 |
NC_000012.12 |
12 |
65304469 |
+ |
MSRB3 |
NNNGATTAGGAAGATTCATG |
NGA |
2 |
0.0648 |
Tier II |
| 9 |
NC_000019.10 |
19 |
5724757 |
+ |
CATSPERD |
NNNGAGTAGGAGGATTCATG |
NTG |
2 |
0.0223 |
Tier II |
| 10 |
NC_000023.11 |
X |
96440585 |
- |
LOC107985714 |
NNNGATTATGGGGATTCATG |
NGG |
2 |
0.3467 |
Tier III |
| 11 |
NC_000004.12 |
4 |
159846253 |
- |
LOC107986324 |
NNNGATTAGAAGTATTCATG |
NGG |
2 |
0.28 |
Tier III |
| 12 |
NC_000009.12 |
9 |
105939219 |
+ |
LOC107987108 |
NNNGATTTGGGGGATTCATG |
NGG |
2 |
0.2786 |
Tier III |
| 13 |
NC_000022.11 |
22 |
35580672 |
+ |
LOC107985590 |
NNNGAATAGGAGGACTCATG |
NGG |
2 |
0.2364 |
Tier III |
| 14 |
NC_000001.11 |
1 |
232866014 |
- |
LOC101927711 |
NNNGATTAGAAGGATTCATG |
NGT |
2 |
0.0151 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000074.6 |
8 |
106041014 |
+ |
Dus2 |
NNNAAATAGGAGGATTCATG |
NGG |
2 |
0.78 |
Tier II |
| 2 |
NC_000071.6 |
5 |
66481999 |
+ |
Apbb2 |
NNNGAAAAGGAGGATTCATG |
NGG |
2 |
0.7583 |
Tier II |
| 3 |
NC_000072.6 |
6 |
23819073 |
+ |
Cadps2 |
NNNGATGAGGAGAATTCATG |
NGG |
2 |
0.543 |
Tier II |
| 4 |
NC_000073.6 |
7 |
109924903 |
- |
Dennd5a |
NNNGATCTGGAGGATTCATG |
NGG |
2 |
0.2946 |
Tier II |
| 5 |
NC_000084.6 |
18 |
72072331 |
+ |
Dcc |
NNNGATTAGGAGGATTCATA |
NTG |
2 |
0.0365 |
Tier II |
| 6 |
NC_000070.6 |
4 |
97808816 |
+ |
Nfia |
NNNAATTAGGAGGATTCATG |
NTG |
2 |
0.0351 |
Tier II |
| 7 |
NC_000072.6 |
6 |
138048481 |
+ |
Slc15a5 |
NNNGATTAGGAGGATTGAAG |
NGG |
2 |
0.0168 |
Tier II |
| 8 |
NC_000077.6 |
11 |
18976253 |
+ |
Meis1 |
NNNGATTAGGAGGAGTCATG |
NAG |
2 |
0.013 |
Tier II |
| 9 |
NC_000078.6 |
12 |
94005141 |
+ |
Gm7119 |
NNNGAGTAGGAGGATTCATG |
NGA |
2 |
0.0397 |
Tier III |
Other clones with same target sequence:
(none)
