Construct: sgRNA BRDN0001147816
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GGTCTCACCTGCACCCCGAA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NO_SITE control
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 80224
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000003.12 |
3 |
112102960 |
+ |
C3orf52 |
NNNCTCACCTGCTCACCGAA |
NGG |
2 |
0.0667 |
Tier I |
| 2 |
NC_000009.12 |
9 |
93075926 |
+ |
SUSD3 |
NNNCTCACCTGCACCTGGAA |
NGG |
2 |
0.0181 |
Tier I |
| 3 |
NC_000020.11 |
20 |
37932079 |
- |
VSTM2L |
NNNCTCACCTGTACCCCGAA |
NGG |
1 |
0.5385 |
Tier II |
| 4 |
NC_000009.12 |
9 |
133776863 |
- |
VAV2 |
NNNCCCACCTCCACCCCGAA |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000017.11 |
17 |
80437549 |
- |
ENDOV |
NNNCACACCTGCACCCCAAA |
NGG |
2 |
0.3462 |
Tier II |
| 6 |
NC_000019.10 |
19 |
7558165 |
- |
PNPLA6 |
NNNCTCACCTGCACCCATAA |
NGG |
2 |
0.3111 |
Tier II |
| 7 |
NC_000019.10 |
19 |
19046058 |
+ |
ARMC6 |
NNNCGCCCCTGCACCCCGAA |
NGG |
2 |
0.28 |
Tier II |
| 8 |
NC_000022.11 |
22 |
37243837 |
- |
RAC2 |
NNNCTCACCTGCACCCCAAA |
NGC |
2 |
0.0154 |
Tier II |
| 9 |
NC_000020.11 |
20 |
10547720 |
- |
SLX4IP |
NNNCTCACCTGCACCCTGAA |
NGC |
2 |
0.0104 |
Tier II |
| 10 |
NC_000003.12 |
3 |
181581334 |
+ |
SOX2-OT |
NNNCTCACATCCACCCCGAA |
NGG |
2 |
0.3673 |
Tier III |
| 11 |
NC_000007.14 |
7 |
17148743 |
- |
LOC101927609 |
NNNCTCACCTGCACCCTGGA |
NGG |
2 |
0.175 |
Tier III |
| 12 |
NC_000007.14 |
7 |
17148743 |
- |
LOC107986772 |
NNNCTCACCTGCACCCTGGA |
NGG |
2 |
0.175 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000070.6 |
4 |
47300606 |
- |
Col15a1 |
NNNCTAACCTGCACCCAGAA |
NGG |
2 |
0.4333 |
Tier I |
| 2 |
NC_000072.6 |
6 |
86629082 |
+ |
Asprv1 |
NNNCGCACCTGCACCCTGAA |
NGG |
2 |
0.2987 |
Tier I |
| 3 |
NC_000068.7 |
2 |
30357338 |
- |
Sh3glb2 |
NNNCTCACCTGCATCCCAAA |
NGG |
2 |
0.5077 |
Tier II |
| 4 |
NC_000068.7 |
2 |
31141194 |
+ |
Fnbp1 |
NNNCCCACCTGCACCCCCAA |
NGG |
2 |
0.4762 |
Tier II |
| 5 |
NC_000068.7 |
2 |
160749134 |
- |
Plcg1 |
NNNCTCACCTACACCCAGAA |
NGG |
2 |
0.4667 |
Tier II |
| 6 |
NC_000073.6 |
7 |
138283833 |
+ |
Tcerg1l |
NNNCTCACCTGCACCCTAAA |
NGG |
2 |
0.3231 |
Tier II |
| 7 |
NC_000072.6 |
6 |
112840058 |
+ |
Srgap3 |
NNNCTCACCTCCACCCCAAA |
NGG |
2 |
0.2967 |
Tier II |
| 8 |
NC_000083.6 |
17 |
30845783 |
+ |
Dnah8 |
NNNCTCACCTTCATCCCGAA |
NGG |
2 |
0.2821 |
Tier II |
| 9 |
NC_000067.6 |
1 |
183308884 |
- |
Aida |
NNNCTCACCTGCAACCCTAA |
NGG |
2 |
0.2333 |
Tier II |
| 10 |
NC_000086.7 |
X |
52085398 |
- |
Gpc4 |
NNNCACACCTGCACCCCGGA |
NGG |
2 |
0.1875 |
Tier II |
| 11 |
NC_000083.6 |
17 |
7053114 |
+ |
Gm1604b |
NNNGTCACCTGCACCCCGAA |
NAG |
2 |
0.1296 |
Tier II |
| 12 |
NC_000074.6 |
8 |
36130777 |
- |
Prag1 |
NNNCTCACCTGCACCCAGAA |
NAG |
2 |
0.121 |
Tier II |
| 13 |
NC_000083.6 |
17 |
29179754 |
- |
Cpne5 |
NNNCTCACCTGCACCCCCAC |
NGG |
2 |
0.1082 |
Tier II |
| 14 |
NC_000069.6 |
3 |
88211486 |
+ |
Gm3764 |
NNNTTCACCTGCACCCCTAA |
NGG |
2 |
0.5333 |
Tier III |
| 15 |
NC_000070.6 |
4 |
47300606 |
- |
Gm52732 |
NNNCTAACCTGCACCCAGAA |
NGG |
2 |
0.4333 |
Tier III |
| 16 |
NC_000076.6 |
10 |
44590043 |
+ |
Gm5950 |
NNNCTCAGCTGCACCCCGAA |
NGC |
2 |
0.0143 |
Tier III |
Other clones with same target sequence:
(none)
