Construct: sgRNA BRDN0001147816

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGTCTCACCTGCACCCCGAA

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NO_SITE control
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
80224

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000003.12 3 112102960 + C3orf52 NNNCTCACCTGCTCACCGAA NGG 2 0.0667 Tier I
2 NC_000009.12 9 93075926 + SUSD3 NNNCTCACCTGCACCTGGAA NGG 2 0.0181 Tier I
3 NC_000020.11 20 37932079 - VSTM2L NNNCTCACCTGTACCCCGAA NGG 1 0.5385 Tier II
4 NC_000009.12 9 133776863 - VAV2 NNNCCCACCTCCACCCCGAA NGG 2 0.4286 Tier II
5 NC_000017.11 17 80437549 - ENDOV NNNCACACCTGCACCCCAAA NGG 2 0.3462 Tier II
6 NC_000019.10 19 7558165 - PNPLA6 NNNCTCACCTGCACCCATAA NGG 2 0.3111 Tier II
7 NC_000019.10 19 19046058 + ARMC6 NNNCGCCCCTGCACCCCGAA NGG 2 0.28 Tier II
8 NC_000022.11 22 37243837 - RAC2 NNNCTCACCTGCACCCCAAA NGC 2 0.0154 Tier II
9 NC_000020.11 20 10547720 - SLX4IP NNNCTCACCTGCACCCTGAA NGC 2 0.0104 Tier II
10 NC_000003.12 3 181581334 + SOX2-OT NNNCTCACATCCACCCCGAA NGG 2 0.3673 Tier III
11 NC_000007.14 7 17148743 - LOC101927609 NNNCTCACCTGCACCCTGGA NGG 2 0.175 Tier III
12 NC_000007.14 7 17148743 - LOC107986772 NNNCTCACCTGCACCCTGGA NGG 2 0.175 Tier III
Download CSV

Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000070.6 4 47300606 - Col15a1 NNNCTAACCTGCACCCAGAA NGG 2 0.4333 Tier I
2 NC_000072.6 6 86629082 + Asprv1 NNNCGCACCTGCACCCTGAA NGG 2 0.2987 Tier I
3 NC_000068.7 2 30357338 - Sh3glb2 NNNCTCACCTGCATCCCAAA NGG 2 0.5077 Tier II
4 NC_000068.7 2 31141194 + Fnbp1 NNNCCCACCTGCACCCCCAA NGG 2 0.4762 Tier II
5 NC_000068.7 2 160749134 - Plcg1 NNNCTCACCTACACCCAGAA NGG 2 0.4667 Tier II
6 NC_000073.6 7 138283833 + Tcerg1l NNNCTCACCTGCACCCTAAA NGG 2 0.3231 Tier II
7 NC_000072.6 6 112840058 + Srgap3 NNNCTCACCTCCACCCCAAA NGG 2 0.2967 Tier II
8 NC_000083.6 17 30845783 + Dnah8 NNNCTCACCTTCATCCCGAA NGG 2 0.2821 Tier II
9 NC_000067.6 1 183308884 - Aida NNNCTCACCTGCAACCCTAA NGG 2 0.2333 Tier II
10 NC_000086.7 X 52085398 - Gpc4 NNNCACACCTGCACCCCGGA NGG 2 0.1875 Tier II
11 NC_000083.6 17 7053114 + Gm1604b NNNGTCACCTGCACCCCGAA NAG 2 0.1296 Tier II
12 NC_000074.6 8 36130777 - Prag1 NNNCTCACCTGCACCCAGAA NAG 2 0.121 Tier II
13 NC_000083.6 17 29179754 - Cpne5 NNNCTCACCTGCACCCCCAC NGG 2 0.1082 Tier II
14 NC_000069.6 3 88211486 + Gm3764 NNNTTCACCTGCACCCCTAA NGG 2 0.5333 Tier III
15 NC_000070.6 4 47300606 - Gm52732 NNNCTAACCTGCACCCAGAA NGG 2 0.4333 Tier III
16 NC_000076.6 10 44590043 + Gm5950 NNNCTCAGCTGCACCCCGAA NGC 2 0.0143 Tier III
Download CSV

Other clones with same target sequence:

(none)

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