Construct: sgRNA BRDN0001147819
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCACCTGAGATAATTGACCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MAP3K15 (389840)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76101
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000023.11 |
X |
19380244 |
- |
MAP3K15 |
NNNCCTGAGATAATTGACCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
123263482 |
+ |
TRDN |
NNNGCTAAGATAATTGACCA |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000004.12 |
4 |
106987344 |
- |
DKK2 |
NNNAATGAGATAATTGACCA |
NGG |
2 |
0.4812 |
Tier II |
| 4 |
NC_000006.12 |
6 |
47862908 |
- |
PTCHD4 |
NNNCCAGATATAATTGACCA |
NGG |
2 |
0.4622 |
Tier II |
| 5 |
NC_000005.10 |
5 |
14845731 |
- |
ANKH |
NNNCCTGTGGTAATTGACCA |
NGG |
2 |
0.2381 |
Tier II |
| 6 |
NC_000003.12 |
3 |
192704791 |
- |
FGF12 |
NNNGCTGAGATAATTGACAA |
NGG |
2 |
0.2143 |
Tier II |
| 7 |
NC_000002.12 |
2 |
40458727 |
- |
SLC8A1 |
NNNCCTGAGATAACTGACAA |
NGG |
2 |
0.1224 |
Tier II |
| 8 |
NC_000009.12 |
9 |
98848374 |
+ |
GALNT12 |
NNNGCTGAGATAATTGAGCA |
NGG |
2 |
0.0667 |
Tier II |
| 9 |
NC_000015.10 |
15 |
78497841 |
- |
IREB2 |
NNNCCTGAGATACCTGACCA |
NGG |
2 |
0.0602 |
Tier II |
| 10 |
NC_000004.12 |
4 |
185953925 |
- |
SORBS2 |
NNNCATGAGATAATGGACCA |
NGG |
2 |
0.0286 |
Tier II |
| 11 |
NC_000002.12 |
2 |
118955915 |
+ |
MARCO |
NNNGCTGAGATAATTCACCA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000003.12 |
3 |
170709689 |
+ |
SLC7A14-AS1 |
NNNCCTAAGATAATTGATCA |
NGG |
2 |
0.6429 |
Tier III |
| 13 |
NC_000003.12 |
3 |
170709689 |
+ |
LOC124906302 |
NNNCCTAAGATAATTGATCA |
NGG |
2 |
0.6429 |
Tier III |
| 14 |
NC_000006.12 |
6 |
106759133 |
- |
LINC02532 |
NNNCCTGGGATAATTGCCCA |
NGG |
2 |
0.1294 |
Tier III |
| 15 |
NC_000020.11 |
20 |
21644368 |
- |
LINC01726 |
NNNCCTGAGATAAGTGAGCA |
NGG |
2 |
0.0 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000086.7 |
X |
160112440 |
+ |
Map3k15 |
NNNCCTGAGATTATTGATCA |
NGG |
2 |
0.2143 |
Tier I |
| 2 |
NC_000073.6 |
7 |
12421746 |
+ |
Zfp551 |
NNNACTGAGATAATAGACCA |
NGG |
2 |
0.4875 |
Tier II |
| 3 |
NC_000074.6 |
8 |
10313922 |
- |
Myo16 |
NNNTCTGAGAGAATTGACCA |
NGG |
2 |
0.32 |
Tier II |
| 4 |
NC_000078.6 |
12 |
84609257 |
- |
Abcd4 |
NNNCCTGAAAGAATTGACCA |
NGG |
2 |
0.2571 |
Tier II |
| 5 |
NC_000086.7 |
X |
141504333 |
- |
Col4a5 |
NNNCCTGAGAGAATTGACTA |
NGG |
2 |
0.1846 |
Tier II |
| 6 |
NC_000072.6 |
6 |
80095281 |
+ |
Lrrtm4 |
NNNCCTGAGATATTTGAACA |
NGG |
2 |
0.1615 |
Tier II |
| 7 |
NC_000076.6 |
10 |
73978303 |
+ |
Pcdh15 |
NNNCCTGAGACAATTGCCCA |
NGG |
2 |
0.1176 |
Tier II |
| 8 |
NC_000079.6 |
13 |
34877816 |
+ |
Prpf4b |
NNNCCTGAGATCATTGAGCA |
NGG |
2 |
0.0351 |
Tier II |
| 9 |
NC_000067.6 |
1 |
173482573 |
+ |
Ifi206 |
NNNCCTGAGATAATGGACCA |
NAG |
2 |
0.013 |
Tier II |
| 10 |
NC_000075.6 |
9 |
90019572 |
- |
Rasgrf1 |
NNNCCTGAGATAATGCACCA |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000086.7 |
X |
164535710 |
+ |
Asb9 |
NNNCCTGAGATAATTCAACA |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000074.6 |
8 |
61307892 |
+ |
Sh3rf1 |
NNNCCTGAGATAATTTACCA |
NGT |
2 |
0.0 |
Tier II |
| 13 |
NC_000074.6 |
8 |
126099001 |
+ |
Gm46072 |
NNNCCTGAGATAATTAACCA |
NAG |
2 |
0.2593 |
Tier III |
| 14 |
NC_000079.6 |
13 |
70077138 |
+ |
Gm3772 |
NNNCCTGAGGTCATTGACCA |
NGG |
2 |
0.1462 |
Tier III |
| 15 |
NC_000081.6 |
15 |
51321732 |
- |
Gm19303 |
NNNCCTGAGATAATTGAACA |
NAG |
2 |
0.1396 |
Tier III |
| 16 |
NC_000068.7 |
2 |
144781296 |
+ |
Gm39951 |
NNNCCTGAGATAATGGACTA |
NGG |
2 |
0.0231 |
Tier III |
| 17 |
NC_000070.6 |
4 |
3671379 |
- |
Gm42250 |
NNNCCTGTGATAATTGACCA |
NTG |
2 |
0.0167 |
Tier III |
| 18 |
NC_000083.6 |
17 |
16431453 |
+ |
Gm41534 |
NNNCCTGAGATAATTGACAA |
NGC |
2 |
0.0095 |
Tier III |
| 19 |
NC_000072.6 |
6 |
68196100 |
+ |
Igk |
NNNCCTGAGATAATTCACCA |
NGG |
1 |
0.0 |
Tier III |
| 20 |
NC_000072.6 |
6 |
68354755 |
+ |
Igk |
NNNCCTGAGATAATTCACCA |
NGG |
1 |
0.0 |
Tier III |
| 21 |
NC_000074.6 |
8 |
61307892 |
+ |
Gm40499 |
NNNCCTGAGATAATTTACCA |
NGT |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
