Construct: sgRNA BRDN0001147825

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
ACTGGAAATCCAGACCAATG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
NRBP2 (340371)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
76453

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000008.11 8 143838751 - NRBP2 NNNGGAAATCCAGACCAATG NGG 0 1.0 Tier I
2 NC_000020.11 20 45941082 + PCIF1 NNNGGACATCCAGACCAATG NTG 2 0.017 Tier I
3 NC_000003.12 3 129402630 + EFCAB12 NNNGGAAATCCAGGCCAATG NGG 1 0.4667 Tier II
4 NC_000017.11 17 63165797 + TANC2 NNNGGAAATCCAGGCCAATA NGG 2 0.4375 Tier II
5 NC_000005.10 5 68265037 - PIK3R1 NNNGAAAATCCAGACCATTG NGG 2 0.4333 Tier II
6 NC_000003.12 3 11648285 + VGLL4 NNNAGAAATCCAGGCCAATG NGG 2 0.42 Tier II
7 NC_000018.10 18 72836479 - NETO1 NNNGAATATCCAGACCAATG NGG 2 0.3792 Tier II
8 NC_000008.11 8 106596858 + OXR1 NNNGGAAATCAAGACCATTG NGG 2 0.375 Tier II
9 NC_000010.11 10 19467158 + MALRD1 NNNGGAAATCAAGACCATTG NGG 2 0.375 Tier II
10 NC_000012.12 12 13834252 - GRIN2B NNNGGAGATCCAGACCATTG NGG 2 0.3529 Tier II
11 NC_000020.11 20 1320344 + SDCBP2 NNNGGGAATCCAGGCCAATG NGG 2 0.3333 Tier II
12 NC_000011.10 11 75295029 + ARRB1 NNNGGAATTCAAGACCAATG NGG 2 0.3214 Tier II
13 NC_000003.12 3 97724571 + EPHA6 NNNGGAGTTCCAGACCAATG NGG 2 0.3025 Tier II
14 NC_000005.10 5 151827074 - GLRA1 NNNGGAGTTCCAGACCAATG NGG 2 0.3025 Tier II
15 NC_000006.12 6 5638370 + FARS2 NNNGGAGTTCCAGACCAATG NGG 2 0.3025 Tier II
16 NC_000013.11 13 100254887 + PCCA NNNGGAGTTCCAGACCAATG NGG 2 0.3025 Tier II
17 NC_000007.14 7 143897225 - TCAF1 NNNGAAAATCCAGACCAACG NGG 2 0.2391 Tier II
18 NC_000005.10 5 38262273 + EGFLAM NNNGGAAATCCAGACAACTG NGG 2 0.1905 Tier II
19 NC_000011.10 11 124135083 + VWA5A NNNGGCAATCCAGACCAAAG NGG 2 0.1299 Tier II
20 NC_000010.11 10 68480711 - SLC25A16 NNNGGAAATCGAGACCATTG NGG 2 0.125 Tier II
21 NC_000009.12 9 134056186 + BRD3 NNNGGAAATCCCGGCCAATG NGG 2 0.1228 Tier II
22 NC_000011.10 11 73231351 + P2RY2 NNNGGAATTCGAGACCAATG NGG 2 0.1071 Tier II
23 NC_000002.12 2 217853406 + TNS1 NNNGGACATCCAGAACAATG NGG 2 0.0972 Tier II
24 NC_000006.12 6 3342237 + SLC22A23 NNNTGAAATCCAGCCCAATG NGG 2 0.0779 Tier II
25 NC_000004.12 4 16014886 + PROM1 NNNGGAATTCCAGACCAATG NTG 2 0.0167 Tier II
26 NC_000008.11 8 14275936 + SGCZ NNNGGAAAGCCAGACCAATG NGT 2 0.01 Tier II
27 NC_000001.11 1 215002738 + LOC124904510 NNNGGAAAACCAAACCAATG NGG 2 0.8571 Tier III
28 NC_000006.12 6 76800793 - LOC105377862 NNNGGAGATCAAGACCAATG NGG 2 0.5294 Tier III
29 NC_000008.11 8 106596858 + OXR1-AS1 NNNGGAAATCAAGACCATTG NGG 2 0.375 Tier III
30 NC_000012.12 12 13834252 - LOC105369667 NNNGGAGATCCAGACCATTG NGG 2 0.3529 Tier III
31 NC_000020.11 20 1320344 + FKBP1A-SDCBP2 NNNGGGAATCCAGGCCAATG NGG 2 0.3333 Tier III
32 NC_000015.10 15 96215038 - NR2F2-AS1 NNNGGAATTCAAGACCAATG NGG 2 0.3214 Tier III
33 NC_000015.10 15 96215038 - LOC112268156 NNNGGAATTCAAGACCAATG NGG 2 0.3214 Tier III
34 NC_000003.12 3 97724571 + LOC124906256 NNNGGAGTTCCAGACCAATG NGG 2 0.3025 Tier III
35 NT_187383.1 16 1786105 - LOC102724066 NNNGGAGTTCCAGACCAATG NGG 2 0.3025 Tier III
36 NC_000007.14 7 143897225 - LOC105375549 NNNGAAAATCCAGACCAACG NGG 2 0.2391 Tier III
37 NC_000002.12 2 187224992 + CALCRL-AS1 NNNGGAAGTCCAGAACAATG NGG 2 0.163 Tier III
38 NC_000003.12 3 168666519 - EGFEM1P NNNGGGAATCCAGAACAATG NGG 2 0.1587 Tier III
39 NC_000003.12 3 168666519 - RPSAP33 NNNGGGAATCCAGAACAATG NGG 2 0.1587 Tier III
40 NC_000009.12 9 96084488 + LOC158435 NNNGGAAGTCCAGACCACTG NGG 2 0.1397 Tier III
41 NC_000009.12 9 134056186 + LOC100130548 NNNGGAAATCCCGGCCAATG NGG 2 0.1228 Tier III
42 NC_000002.12 2 64447928 - LGALSL-DT NNNGGAAATCCTGACTAATG NGG 2 0.1026 Tier III
43 NC_000002.12 2 87520713 - CYTOR NNNGGAATTCCAGCCCAATG NGG 2 0.0918 Tier III
44 NC_000002.12 2 87520713 - NCAL1 NNNGGAATTCCAGCCCAATG NGG 2 0.0918 Tier III
45 NC_000002.12 2 111430115 + MIR4435-2HG NNNGGAATTCCAGCCCAATG NGG 2 0.0918 Tier III
46 NC_000006.12 6 139851881 - FILNC1 NNNGGAATTCCAGATCAATG NGG 2 0.0286 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000068.7 2 164885937 + Pcif1 NNNGGACATCCAGACCAATG NTG 2 0.017 Tier I
2 NC_000068.7 2 49926690 + Lypd6b NNNGGAAAACCAGACCAATG NGG 1 0.9286 Tier II
3 NC_000069.6 3 138809796 - Tspan5 NNNGGAAATAAAGACCAATG NGG 2 0.65 Tier II
4 NC_000079.6 13 8674891 + Adarb2 NNNGAAAATCCAGACCATTG NGG 2 0.4333 Tier II
5 NC_000067.6 1 21215990 + Tmem14a NNNGGGATTCCAGACCAATG NGG 2 0.3061 Tier II
6 NC_000071.6 5 81188971 - Adgrl3 NNNGGAAATCAAGACCAGTG NGG 2 0.3 Tier II
7 NC_000073.6 7 20037704 - Nlrp9b NNNGGAAAACCAGACCAAAG NGG 2 0.2653 Tier II
8 NC_000081.6 15 48079071 + Csmd3 NNNGGAAGTCCAGACCAATG NAG 2 0.1901 Tier II
9 NC_000080.6 14 12157911 - Ptprg NNNGGAACTCCACACCAATG NGG 2 0.1805 Tier II
10 NC_000070.6 4 154227482 - Megf6 NNNTGAATTCCAGACCAATG NGG 2 0.1558 Tier II
11 NC_000073.6 7 144334204 + Shank2 NNNTGAATTCCAGACCAATG NGG 2 0.1558 Tier II
12 NC_000071.6 5 151133618 + Stard13 NNNGGAAATCCAGGCCAAGG NGG 2 0.1167 Tier II
13 NC_000069.6 3 126641680 + Camk2d NNNGGAAATCCAGTCCACTG NGG 2 0.1016 Tier II
14 NC_000075.6 9 27951229 + Opcml NNNGGAAATGCAGAACAATG NGG 2 0.0864 Tier II
15 NC_000081.6 15 93522339 - Prickle1 NNNGGAAATCAAGAGCAATG NGG 2 0.0375 Tier II
16 NC_000083.6 17 81399730 + Slc8a1 NNNGGAAATCCAGGGCAATG NGG 2 0.0233 Tier II
17 NC_000078.6 12 108237685 + Ccdc85c NNNGGAAATCCAGACCAAGG NGA 2 0.0174 Tier II
18 NC_000067.6 1 21215990 + Gm28836 NNNGGGATTCCAGACCAATG NGG 2 0.3061 Tier III
19 NC_000068.7 2 136458311 + Gm32546 NNNGGAAAACCAGACCAAAG NGG 2 0.2653 Tier III
20 NC_000074.6 8 104095187 + Gm29682 NNNGGAAAACCAGACCAAGG NGG 2 0.2321 Tier III
21 NC_000071.6 5 31599944 - Gm38424 NNNGGAGATCCAGACCAATG NGA 2 0.049 Tier III
22 NC_000073.6 7 38804680 - 1700010N08Rik NNNGGAAATCCAGATCAATG NGA 2 0.0046 Tier III
23 NC_000073.6 7 38940934 - Gm32110 NNNGGAAATCCAGATCAATG NGA 2 0.0046 Tier III
24 NC_000073.6 7 39296106 - Gm33382 NNNGGAAATCCAGATCAATG NGA 2 0.0046 Tier III
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Other clones with same target sequence:

(none)