Construct: sgRNA BRDN0001147831
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGTGATCTCAACAACAGTAC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- MARK3 (4140)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77718
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000014.9 |
14 |
103468110 |
+ |
MARK3 |
NNNGATCTCAACAACAGTAC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000003.12 |
3 |
51054308 |
- |
DOCK3 |
NNNCATCTCAACAACAATAC |
NGG |
2 |
0.4941 |
Tier II |
| 3 |
NC_000009.12 |
9 |
122521796 |
- |
OR1J2 |
NNNGATCTGAACAACAGAAC |
NGG |
2 |
0.4127 |
Tier II |
| 4 |
NC_000017.11 |
17 |
64353121 |
+ |
PECAM1 |
NNNGATTTCAACAACAGTAA |
NGG |
2 |
0.4063 |
Tier II |
| 5 |
NC_000010.11 |
10 |
50545393 |
+ |
SGMS1 |
NNNGTTCCCAACAACAGTAC |
NGG |
2 |
0.3636 |
Tier II |
| 6 |
NC_000022.11 |
22 |
33487650 |
- |
LARGE1 |
NNNCATCTCAACAACAGCAC |
NGG |
2 |
0.2269 |
Tier II |
| 7 |
NC_000020.11 |
20 |
50889521 |
- |
ADNP |
NNNCTTCTCAACAACAGTAC |
NGG |
2 |
0.1925 |
Tier II |
| 8 |
NC_000005.10 |
5 |
32065617 |
+ |
PDZD2 |
NNNGATGTCAACAACAGTGC |
NGG |
2 |
0.1765 |
Tier II |
| 9 |
NC_000001.11 |
1 |
166862483 |
- |
TADA1 |
NNNGATCTCAACTTCAGTAC |
NGG |
2 |
0.16 |
Tier II |
| 10 |
NC_000011.10 |
11 |
11480598 |
- |
GALNT18 |
NNNGATCTCAAAACCAGTAC |
NGG |
2 |
0.1531 |
Tier II |
| 11 |
NC_000023.11 |
X |
38614293 |
- |
TSPAN7 |
NNNGAACTCAACAACAGTAC |
NTG |
2 |
0.0338 |
Tier II |
| 12 |
NC_000014.9 |
14 |
66796689 |
- |
GPHN |
NNNGAGCTCAACAACTGTAC |
NGG |
2 |
0.0 |
Tier II |
| 13 |
NC_000005.10 |
5 |
117523221 |
+ |
LINC00992 |
NNNGATCACACCAACAGTAC |
NGG |
2 |
0.32 |
Tier III |
| 14 |
NC_000012.12 |
12 |
89719420 |
- |
LOC107984543 |
NNNTATCACAACAACAGTAC |
NGG |
2 |
0.2909 |
Tier III |
| 15 |
NC_000011.10 |
11 |
11480598 |
- |
LOC107983976 |
NNNGATCTCAAAACCAGTAC |
NGG |
2 |
0.1531 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000078.6 |
12 |
52843202 |
- |
Akap6 |
NNNGATCTCTACAACAGTTC |
NGG |
2 |
0.4751 |
Tier II |
| 2 |
NC_000074.6 |
8 |
73329798 |
+ |
Large1 |
NNNGATGTCAACAACAGTAA |
NGG |
2 |
0.2353 |
Tier II |
| 3 |
NC_000076.6 |
10 |
18235560 |
- |
Ccdc28a |
NNNGATCTCAACAACATAAC |
NGG |
2 |
0.1667 |
Tier II |
| 4 |
NC_000067.6 |
1 |
6588595 |
+ |
St18 |
NNNGATCTCACCAACAGTAT |
NGG |
2 |
0.12 |
Tier II |
| 5 |
NC_000080.6 |
14 |
39230406 |
- |
Nrg3 |
NNNGATCTCAACAGCAGTAC |
NGA |
2 |
0.0324 |
Tier II |
| 6 |
NC_000068.7 |
2 |
119168460 |
- |
Gchfr |
NNNGATTTCAACAACTGTAC |
NGG |
2 |
0.0 |
Tier II |
| 7 |
NC_000080.6 |
14 |
39230406 |
- |
Gm20642 |
NNNGATCTCAACAGCAGTAC |
NGA |
2 |
0.0324 |
Tier III |
Other clones with same target sequence:
(none)
