Construct: sgRNA BRDN0001147832

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
CCTGGCTAAGATCTTCGAGC

Original Target:

Taxon:
Homo sapiens (human)
Gene:
COQ8A (56997)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77321

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 226982717 + COQ8A NNNGGCTAAGATCTTCGAGC NGG 0 1.0 Tier I
2 NC_000009.12 9 125306793 - GAPVD1 NNNGTCTAAGATCTTGGAGC NGG 2 0.0462 Tier II
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000085.6 19 38069162 + Cep55 NNNAGCAAAGATCTTCGAGC NGG 2 0.7875 Tier I
2 NC_000067.6 1 180170390 - Coq8a NNNGGCCAAGATCTTTGAGC NGG 2 0.2115 Tier I
3 NC_000068.7 2 75485091 - Gm50595 NNNGGCTAAGATCTCCGAGC NGC 2 0.0061 Tier II
4 NC_000073.6 7 25461017 + 4732471J01Rik NNNGGCTGAGATCTTCGAGC NGA 2 0.0509 Tier III
5 NC_000073.6 7 25515354 + 4732471J01Rik NNNGGCTGAGATCTTCGAGC NGA 2 0.0509 Tier III
6 NC_000068.7 2 33544150 - Gm33642 NNNGGATAAGATCTGCGAGC NGG 2 0.0464 Tier III
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Other clones with same target sequence:

(none)