Construct: sgRNA BRDN0001147836

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
TGGAGCTGATATGAATCTAG

Original Target:

Taxon:
Homo sapiens (human)
Gene:
FPGT-TNNI3K (100526835)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
75904

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000001.11 1 74354055 + TNNI3K NNNAGCTGATATGAATCTAG NGG 0 1.0 Tier I
2 NC_000001.11 1 74354055 + FPGT-TNNI3K NNNAGCTGATATGAATCTAG NGG 0 1.0 Tier I
3 NC_000005.10 5 112738417 + APC NNNGGCTGATGTGAATCTAG NGG 2 0.4063 Tier I
4 NC_000001.11 1 77292363 + AK5 NNNAGCTAAAATGAATCTAG NGG 2 0.8571 Tier II
5 NC_000001.11 1 237755795 - RYR2 NNNAGCTGATATGAATCAAA NGG 2 0.625 Tier II
6 NC_000004.12 4 64369309 - TECRL NNNAGCTAATATGAATATAG NGG 2 0.4667 Tier II
7 NC_000007.14 7 97118724 + SDHAF3 NNNAGCTGATATGAAAATAG NGG 2 0.4242 Tier II
8 NC_000004.12 4 55523753 - CLOCK NNNAGCTGATATGAAATTAG NGG 2 0.4242 Tier II
9 NC_000004.12 4 113281911 - ANK2 NNNAGCTTATATGAATCTTG NGG 2 0.3365 Tier II
10 NC_000023.11 X 53190794 - KDM5C NNNAGCTGATAGGAACCTAG NGG 2 0.3333 Tier II
11 NC_000002.12 2 134185630 - MGAT5 NNNCACTGATATGAATCTAG NGG 2 0.3059 Tier II
12 NC_000009.12 9 109409459 + PTPN3 NNNAGGTGCTATGAATCTAG NGG 2 0.2857 Tier II
13 NC_000010.11 10 23919807 + KIAA1217 NNNAGCTGACCTGAATCTAG NGG 2 0.2133 Tier II
14 NC_000002.12 2 152564904 - FMNL2 NNNAGCTGATATGACTCAAG NGG 2 0.1818 Tier II
15 NC_000001.11 1 245978465 + SMYD3 NNNAGCTGATCTGAATCCAG NGG 2 0.1714 Tier II
16 NC_000018.10 18 744872 - YES1 NNNAGCTGTTATGAATCTAG NGT 2 0.0097 Tier II
17 NC_000006.12 6 42438691 - TRERF1 NNNAGCTGATATGAATCTAG NCT 2 0.0 Tier II
18 NC_000013.11 13 84394753 - LINC00333 NNNAGCTGAAATGAATTTAG NGG 2 0.4 Tier III
19 NC_000003.12 3 61352177 + LOC105377114 NNNAACTGATATGATTCTAG NGG 2 0.1733 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 112160014 - Glipr1l3 NNNAGCTGATATGAATCAAG NGG 1 0.6667 Tier I
2 NC_000076.6 10 112060503 + Glipr1l1 NNNAGCTGATATGAATCAAC NGG 2 0.2857 Tier I
3 NC_000084.6 18 42651226 - Ppp2r2b NNNAGCTGATATGAGACTAG NGG 2 0.5909 Tier II
4 NC_000069.6 3 36200097 + Qrfpr NNNAGCTGAGATGAAACTAG NGG 2 0.4545 Tier II
5 NC_000071.6 5 111177192 - Ttc28 NNNAGCTTGTATGAATCTAG NGG 2 0.4167 Tier II
6 NC_000068.7 2 113411566 - Fmn1 NNNAGCTCATGTGAATCTAG NGG 2 0.4 Tier II
7 NC_000076.6 10 14498374 - Adgrg6 NNNAGCTGCTATGAACCTAG NGG 2 0.381 Tier II
8 NC_000075.6 9 75237344 + Myo5c NNNAGCTGAAATGAATCCAG NGG 2 0.3673 Tier II
9 NC_000076.6 10 28508999 + Ptprk NNNAGCTGAGATGAATCTAT NGG 2 0.35 Tier II
10 NC_000069.6 3 152795096 - Pigk NNNAGCTGATATGAACATAG NGG 2 0.3111 Tier II
11 NC_000073.6 7 90990745 + Dlg2 NNNAGCTGATATGAACATAG NGG 2 0.3111 Tier II
12 NC_000079.6 13 41388353 + Nedd9 NNNAGCTGATATGAACATAG NGG 2 0.3111 Tier II
13 NC_000079.6 13 81714079 + Mblac2 NNNAGCTGATATGAACATAG NGG 2 0.3111 Tier II
14 NC_000080.6 14 115523389 - Gpc5 NNNAGCTGATATGAACATAG NGG 2 0.3111 Tier II
15 NC_000070.6 4 113747717 + Skint5 NNNGGCTGATATGAATATAG NGG 2 0.2917 Tier II
16 NC_000083.6 17 34033830 - Rxrb NNNATCTGATATGAATCTAA NGG 2 0.2813 Tier II
17 NC_000067.6 1 3282136 - Xkr4 NNNAGCTGCTATGAATATAG NGG 2 0.2667 Tier II
18 NC_000070.6 4 112973974 - Skint6 NNNAGCTGCTATGAATATAG NGG 2 0.2667 Tier II
19 NC_000079.6 13 108784429 - Pde4d NNNAGCTGCTATGAATATAG NGG 2 0.2667 Tier II
20 NC_000082.6 16 45422055 + Gm609 NNNAGCTGCTATGAATATAG NGG 2 0.2667 Tier II
21 NC_000076.6 10 14696998 - Vta1 NNNAGGTGATATGAATATAG NGG 2 0.2333 Tier II
22 NC_000067.6 1 69579589 + Ikzf2 NNNAACTGATATGATTCTAG NGG 2 0.1733 Tier II
23 NC_000074.6 8 114821564 - Wwox NNNAGCTGATATGAATCAAG NAG 2 0.1728 Tier II
24 NC_000082.6 16 45738242 - Abhd10 NNNATCTGATATGAATCTTG NGG 2 0.1615 Tier II
25 NC_000072.6 6 46954621 + Cntnap2 NNNATCTGATATGTATCTAG NGG 2 0.16 Tier II
26 NC_000068.7 2 126394139 + Atp8b4 NNNAGCTGACATGAATCTAG NAG 2 0.1383 Tier II
27 NC_000076.6 10 5837166 - Rgs17 NNNAGCTGTTATGAATGTAG NGG 2 0.0353 Tier II
28 NC_000071.6 5 102276456 + Arhgap24 NNNAGCTGATATGAATATAG NTG 2 0.0182 Tier II
29 NC_000069.6 3 76335087 - Fstl5 NNNAGCTGATATTAATCTAG NTG 2 0.0117 Tier II
30 NC_000075.6 9 75237344 + Gm33750 NNNAGCTGAAATGAATCCAG NGG 2 0.3673 Tier III
31 NC_000071.6 5 108876542 - Vmn2r-ps21 NNNAGCTGATATGAACATAG NGG 2 0.3111 Tier III
32 NC_000078.6 12 114483669 - Igh NNNAGCTGATATGAACATAG NGG 2 0.3111 Tier III
33 NC_000078.6 12 114535011 - Igh NNNAGCTGATATGAACATAG NGG 2 0.3111 Tier III
34 NC_000079.6 13 78543241 + Gm32067 NNNAGCTGGTATGAATTTAG NGG 2 0.3111 Tier III
35 NC_000085.6 19 19936346 + Gm32469 NNNAGCTGCTATGAATATAG NGG 2 0.2667 Tier III
36 NC_000072.6 6 46954621 + Gm38801 NNNATCTGATATGTATCTAG NGG 2 0.16 Tier III
37 NC_000086.7 X 81552744 - LOC115489112 NNNAGCTGATATGAATATAG NTG 2 0.0182 Tier III
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Other clones with same target sequence:

(none)