Construct: sgRNA BRDN0001147838
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CAGAGCCACATAGACGGGGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PRKAG1 (5571)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77745
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000012.12 |
12 |
49003826 |
+ |
PRKAG1 |
NNNAGCCACATAGACGGGGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000019.10 |
19 |
8262114 |
+ |
CERS4 |
NNNAGCCACATAGCCGGGCG |
NGG |
2 |
0.0961 |
Tier I |
| 3 |
NC_000003.12 |
3 |
143309922 |
- |
SLC9A9 |
NNNAGCAACATAGACGAGGG |
NGG |
2 |
0.7 |
Tier II |
| 4 |
NC_000017.11 |
17 |
11384814 |
+ |
SHISA6 |
NNNAGCCAGATAGACGAGGG |
NGG |
2 |
0.5778 |
Tier II |
| 5 |
NC_000009.12 |
9 |
114803139 |
- |
TNFSF15 |
NNNAGCCACAGAGACAGGGG |
NGG |
2 |
0.4 |
Tier II |
| 6 |
NC_000016.10 |
16 |
23838101 |
- |
PRKCB |
NNNAGCCCCAAAGACGGGGG |
NGG |
2 |
0.3214 |
Tier II |
| 7 |
NC_000001.11 |
1 |
245423378 |
+ |
KIF26B |
NNNAGCCACATAGCCGGGAG |
NGG |
2 |
0.1531 |
Tier II |
| 8 |
NC_000005.10 |
5 |
95907246 |
+ |
ELL2 |
NNNAGCCACAAAGAGGGGGG |
NGG |
2 |
0.0375 |
Tier II |
| 9 |
NC_000012.12 |
12 |
49003826 |
+ |
DDN-AS1 |
NNNAGCCACATAGACGGGGG |
NGG |
0 |
1.0 |
Tier III |
| 10 |
NC_000004.12 |
4 |
11372752 |
- |
RNPS1P1 |
NNNAGCCACATAGGCGGTGG |
NGG |
2 |
0.3111 |
Tier III |
| 11 |
NC_000004.12 |
4 |
72965829 |
+ |
LOC105377273 |
NNNAGCAACATAGATGGGGG |
NGG |
2 |
0.05 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
84110320 |
- |
Dysf |
NNNAGCCACATAGACAGGAG |
NGG |
2 |
0.7143 |
Tier II |
| 2 |
NC_000085.6 |
19 |
41203085 |
+ |
Tll2 |
NNNAGCCACATAGACAGGAG |
NGG |
2 |
0.7143 |
Tier II |
| 3 |
NC_000071.6 |
5 |
48536554 |
+ |
Kcnip4 |
NNNACCCACTTAGACGGGGG |
NGG |
2 |
0.6933 |
Tier II |
| 4 |
NC_000073.6 |
7 |
66744497 |
- |
Cers3 |
NNNAGACAGATAGACGGGGG |
NGG |
2 |
0.5748 |
Tier II |
| 5 |
NC_000083.6 |
17 |
67335789 |
+ |
Ptprm |
NNNAGCAACAAAGACGGGGG |
NGG |
2 |
0.5625 |
Tier II |
| 6 |
NC_000068.7 |
2 |
155800195 |
+ |
Mmp24 |
NNNAGCCACAAAGACGGGTG |
NGG |
2 |
0.5 |
Tier II |
| 7 |
NC_000082.6 |
16 |
4603808 |
+ |
Glis2 |
NNNAGCCACATAGCCAGGGG |
NGG |
2 |
0.2143 |
Tier II |
| 8 |
NC_000084.6 |
18 |
5630980 |
+ |
Zeb1 |
NNNATCCACAGAGACGGGGG |
NGG |
2 |
0.12 |
Tier II |
| 9 |
NC_000079.6 |
13 |
108981307 |
+ |
Pde4d |
NNNAGCCACAGAGAAGGGGG |
NGG |
2 |
0.0889 |
Tier II |
| 10 |
NC_000072.6 |
6 |
125151165 |
- |
Iffo1 |
NNNAGCCACATAGACCTGGG |
NGG |
2 |
0.0 |
Tier II |
| 11 |
NC_000075.6 |
9 |
107861561 |
- |
Rbm6 |
NNNAGCCACATAGACTGAGG |
NGG |
2 |
0.0 |
Tier II |
| 12 |
NC_000072.6 |
6 |
86499477 |
+ |
Gm52866 |
NNNACCCACATACACGGGGG |
NGG |
2 |
0.3308 |
Tier III |
| 13 |
NC_000070.6 |
4 |
141886639 |
+ |
Fhad1os2 |
NNNAGCCACATAGGGGGGGG |
NGG |
2 |
0.0233 |
Tier III |
Other clones with same target sequence:
(none)
