Construct: sgRNA BRDN0001147844
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- GCTGCCTGATTCCTAATCAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PI4K2B (55300)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77132
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000004.12 |
4 |
25255191 |
+ |
PI4K2B |
NNNGCCTGATTCCTAATCAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000004.12 |
4 |
51910655 |
+ |
DCUN1D4 |
NNNACCTGATTCCTAATCAT |
NGG |
2 |
0.63 |
Tier II |
| 3 |
NC_000012.12 |
12 |
119715818 |
- |
CIT |
NNNGCCTGATTCCTAATCAC |
NGG |
1 |
0.4286 |
Tier II |
| 4 |
NC_000013.11 |
13 |
74228611 |
+ |
KLF12 |
NNNGTCTGATTCCAAATCAG |
NGG |
2 |
0.3939 |
Tier II |
| 5 |
NC_000008.11 |
8 |
76859281 |
+ |
ZFHX4 |
NNNGCCTGCTTCCTAAACAG |
NGG |
2 |
0.3048 |
Tier II |
| 6 |
NC_000007.14 |
7 |
158344825 |
- |
PTPRN2 |
NNNGCCTGATTTCTCATCAG |
NGG |
2 |
0.1469 |
Tier II |
| 7 |
NC_000005.10 |
5 |
167701718 |
+ |
TENM2 |
NNNGCCTGATTCCTAGTTAG |
NGG |
2 |
0.1236 |
Tier II |
| 8 |
NC_000007.14 |
7 |
133905447 |
- |
EXOC4 |
NNNGCCTGATTCCTAATGAG |
NAG |
2 |
0.0346 |
Tier II |
| 9 |
NC_000003.12 |
3 |
66260986 |
- |
SLC25A26 |
NNNTCCTGATTCCTAATCAG |
NGA |
2 |
0.0253 |
Tier II |
| 10 |
NC_000003.12 |
3 |
11642366 |
- |
VGLL4 |
NNNGCCTGTTTCCTAATCAG |
NTG |
2 |
0.0234 |
Tier II |
| 11 |
NC_000023.11 |
X |
68253446 |
- |
OPHN1 |
NNNGCCTGATTCCTAGTCAG |
NTG |
2 |
0.0075 |
Tier II |
| 12 |
NC_000017.11 |
17 |
5223884 |
- |
SCIMP |
NNNGCCTGATTCCTAAGCAG |
NTG |
2 |
0.0046 |
Tier II |
| 13 |
NC_000004.12 |
4 |
6153234 |
+ |
JAKMIP1 |
NNNCCCTGATTCCTACTCAG |
NGG |
2 |
0.0 |
Tier II |
| 14 |
NC_000004.12 |
4 |
48766464 |
+ |
FRYL |
NNNGCCTGATGCCTACTCAG |
NGG |
2 |
0.0 |
Tier II |
| 15 |
NC_000003.12 |
3 |
9137717 |
- |
SRGAP3 |
NNNGCCTGCTTCCTATTCAG |
NGG |
2 |
0.0 |
Tier II |
| 16 |
NC_000005.10 |
5 |
72634132 |
- |
TNPO1-DT |
NNNTCCTGATTCCTGATCAG |
NGG |
2 |
0.2364 |
Tier III |
| 17 |
NC_000013.11 |
13 |
40921412 |
+ |
SUGT1P3 |
NNNGCCTGAGTCCTAATCAG |
NCG |
2 |
0.0536 |
Tier III |
| 18 |
NC_000013.11 |
13 |
40921412 |
+ |
TPTE2P5 |
NNNGCCTGAGTCCTAATCAG |
NCG |
2 |
0.0536 |
Tier III |
| 19 |
NC_000006.12 |
6 |
44784347 |
+ |
LOC101929770 |
NNNGACTGATTCCTAATCAG |
NGA |
2 |
0.0397 |
Tier III |
| 20 |
NC_000017.11 |
17 |
6156991 |
+ |
LOC105371508 |
NNNGCCTGATTCCTAATCAG |
NTG |
1 |
0.039 |
Tier III |
| 21 |
NC_000011.10 |
11 |
122286338 |
+ |
MIR100HG |
NNNGCCTGATTCATAATCAG |
NGC |
2 |
0.0085 |
Tier III |
| 22 |
NC_000017.11 |
17 |
5223884 |
- |
ZNF594-DT |
NNNGCCTGATTCCTAAGCAG |
NTG |
2 |
0.0046 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000071.6 |
5 |
52750734 |
+ |
Pi4k2b |
NNNGCCTGCTTCCTAACCAG |
NGG |
2 |
0.4034 |
Tier I |
| 2 |
NC_000077.6 |
11 |
94598877 |
+ |
Acsf2 |
NNNGCCTGTATCCTAATCAG |
NGG |
2 |
0.5143 |
Tier II |
| 3 |
NC_000078.6 |
12 |
67071238 |
- |
Mdga2 |
NNNGTCTGATTCTTAATCAG |
NGG |
2 |
0.4455 |
Tier II |
| 4 |
NC_000085.6 |
19 |
3606002 |
- |
Lrp5 |
NNNGCCTGAGTCCTAAACAG |
NGG |
2 |
0.2667 |
Tier II |
| 5 |
NC_000074.6 |
8 |
12763930 |
- |
Atp11a |
NNNGCCTGAATCCTTATCAG |
NGG |
2 |
0.1714 |
Tier II |
| 6 |
NC_000074.6 |
8 |
88659178 |
- |
Nod2 |
NNNGCCTGCTTCCTAGTCAG |
NGG |
2 |
0.1099 |
Tier II |
| 7 |
NC_000070.6 |
4 |
133522168 |
+ |
Kdf1 |
NNNGTCTGATTCCTAAGCAG |
NGG |
2 |
0.0749 |
Tier II |
| 8 |
NC_000074.6 |
8 |
128236324 |
- |
A930035F09Rik |
NNNGCCTGATTCCTAAACAA |
NGG |
2 |
0.5 |
Tier III |
| 9 |
NC_000068.7 |
2 |
51723497 |
+ |
Gm13490 |
NNNGCCTGCTTCCTAGTCAG |
NGG |
2 |
0.1099 |
Tier III |
Other clones with same target sequence:
(none)
