Construct: sgRNA BRDN0001147849
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ATGGCGTGGCGAAAAAGTGG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- BMPR1A (657)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77959
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
86917231 |
+ |
BMPR1A |
NNNGCGTGGCGAAAAAGTGG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000006.12 |
6 |
129158350 |
- |
LAMA2 |
NNNGCATGGCGAAAGAGTGG |
NGG |
2 |
0.65 |
Tier II |
| 3 |
NC_000023.11 |
X |
49190094 |
- |
SYP |
NNNGGGTGGCTAAAAAGTGG |
NGG |
2 |
0.2308 |
Tier II |
| 4 |
NC_000007.14 |
7 |
129502957 |
+ |
SMKR1 |
NNNGCGTGGCGAAAAGATGG |
NGG |
2 |
0.1795 |
Tier II |
| 5 |
NC_000002.12 |
2 |
199467347 |
+ |
SATB2 |
NNNGCGTGGCGAAAAAGGGG |
NAG |
2 |
0.0864 |
Tier II |
| 6 |
NC_000011.10 |
11 |
121362677 |
- |
BMPR1AP2 |
NNNGCGTGGCGAAAAAGTGG |
NGG |
0 |
1.0 |
Tier III |
| 7 |
NC_000006.12 |
6 |
129158350 |
- |
BMPR1AP1 |
NNNGCATGGCGAAAGAGTGG |
NGG |
2 |
0.65 |
Tier III |
| 8 |
NC_000005.10 |
5 |
97608290 |
+ |
LINC01340 |
NNNGGGTGGCGAAAAAGTGG |
NTG |
2 |
0.0234 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
34425042 |
- |
Bmpr1a |
NNNGCGTGGTGAAAAAGTGG |
NTG |
2 |
0.0367 |
Tier I |
| 2 |
NC_000074.6 |
8 |
95853265 |
+ |
Slc38a7 |
NNNGCCTGGCGAAAAAGCGG |
NGG |
2 |
0.2922 |
Tier II |
| 3 |
NC_000086.7 |
X |
88046211 |
+ |
Il1rapl1 |
NNNGAGTGGGGAAAAAGTGG |
NGG |
2 |
0.2222 |
Tier II |
| 4 |
NC_000078.6 |
12 |
82376617 |
+ |
Sipa1l1 |
NNNGCCTGGCGAAACAGTGG |
NGG |
2 |
0.186 |
Tier II |
Other clones with same target sequence:
(none)
