Construct: sgRNA BRDN0001147851
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- TAAAGGTGTCCAGATACGCA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PDGFRL (5157)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 77696
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000008.11 |
8 |
17589733 |
- |
PDGFRL |
NNNAGGTGTCCAGATACGCA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
18892448 |
+ |
ADAMTSL1 |
NNNAGGTGTCCAGACCCGCA |
NGG |
2 |
0.0 |
Tier I |
| 3 |
NC_000009.12 |
9 |
38015043 |
+ |
SHB |
NNNAAGTGTCCAGATACACA |
NGG |
2 |
0.6 |
Tier II |
| 4 |
NC_000014.9 |
14 |
50374561 |
+ |
CDKL1 |
NNNAGGTTTCCAGATAAGCA |
NGG |
2 |
0.2917 |
Tier II |
| 5 |
NC_000011.10 |
11 |
49187109 |
+ |
FOLH1 |
NNNAGGTGTCCAGATACCCT |
NGG |
2 |
0.2857 |
Tier II |
| 6 |
NC_000004.12 |
4 |
10702256 |
+ |
CLNK |
NNNAGGTGTCCAGATACTAA |
NGG |
2 |
0.2857 |
Tier II |
| 7 |
NC_000011.10 |
11 |
12269826 |
- |
MICAL2 |
NNNAGGTGTGCAGATACACA |
NGG |
2 |
0.2692 |
Tier II |
| 8 |
NC_000017.11 |
17 |
37933225 |
- |
TBC1D3K |
NNNAGGTGTCCAGATACACA |
NAG |
2 |
0.1795 |
Tier II |
| 9 |
NC_000017.11 |
17 |
37980302 |
+ |
TBC1D3L |
NNNAGGTGTCCAGATACACA |
NAG |
2 |
0.1795 |
Tier II |
| 10 |
NC_000017.11 |
17 |
38012780 |
- |
TBC1D3D |
NNNAGGTGTCCAGATACACA |
NAG |
2 |
0.1795 |
Tier II |
| 11 |
NC_000017.11 |
17 |
38059840 |
+ |
TBC1D3C |
NNNAGGTGTCCAGATACACA |
NAG |
2 |
0.1795 |
Tier II |
| 12 |
NC_000017.11 |
17 |
38136722 |
- |
TBC1D3E |
NNNAGGTGTCCAGATACACA |
NAG |
2 |
0.1795 |
Tier II |
| 13 |
NC_000007.14 |
7 |
24345014 |
+ |
LOC107986777 |
NNNAGCTGTCCAGATACACA |
NGG |
2 |
0.472 |
Tier III |
| 14 |
NC_000008.11 |
8 |
125610645 |
- |
LINC02964 |
NNNAGGTGGCCAGATACCCA |
NGG |
2 |
0.2948 |
Tier III |
| 15 |
NC_000009.12 |
9 |
106392799 |
+ |
LOC107987108 |
NNNAGGTGTCCAGATACCCA |
NGT |
2 |
0.0077 |
Tier III |
| 16 |
NC_000022.11 |
22 |
21065506 |
+ |
TUBA3GP |
NNNAGGTGTCCAGATAGGCA |
NCG |
2 |
0.0063 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000085.6 |
19 |
32732035 |
- |
Atad1 |
NNNCAGTGTCCAGATACGCA |
NGG |
2 |
0.3059 |
Tier II |
| 2 |
NC_000080.6 |
14 |
57959884 |
+ |
Micu2 |
NNNAGGTGTCCAGATACAAA |
NGG |
2 |
0.2967 |
Tier II |
| 3 |
NC_000068.7 |
2 |
162523739 |
+ |
Ptprt |
NNNAGGTGTCCACATACCCA |
NGG |
2 |
0.2005 |
Tier II |
| 4 |
NC_000071.6 |
5 |
150316125 |
+ |
Fry |
NNNAGGTGTCAAGATAGGCA |
NGG |
2 |
0.0441 |
Tier II |
| 5 |
NC_000077.6 |
11 |
30969894 |
- |
Asb3 |
NNNAGGTGTCCAGGTAGGCA |
NGG |
2 |
0.0275 |
Tier II |
Other clones with same target sequence:
(none)
