Construct: sgRNA BRDN0001147854
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- AGAATATACTAGAACATCCC
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PIK3R3 (8503)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76343
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000001.11 |
1 |
46066059 |
- |
PIK3R3 |
NNNATATACTAGAACATCCC |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000001.11 |
1 |
46066059 |
- |
P3R3URF-PIK3R3 |
NNNATATACTAGAACATCCC |
NGG |
0 |
1.0 |
Tier I |
| 3 |
NC_000015.10 |
15 |
73741855 |
- |
INSYN1 |
NNNAAATACTAGCACATCCC |
NGG |
2 |
0.1053 |
Tier II |
| 4 |
NC_000020.11 |
20 |
49831919 |
- |
SLC9A8 |
NNNAAATACTAGAACAGCCC |
NGG |
2 |
0.0588 |
Tier II |
| 5 |
NC_000007.14 |
7 |
129616276 |
- |
NRF1 |
NNNATATACTAGAACATCTC |
NGT |
2 |
0.0074 |
Tier II |
| 6 |
NC_000008.11 |
8 |
126817048 |
+ |
LOC105375751 |
NNNATATACAAGATCATCCC |
NGG |
2 |
0.4571 |
Tier III |
| 7 |
NC_000008.11 |
8 |
126817048 |
+ |
LOC105375753 |
NNNATATACAAGATCATCCC |
NGG |
2 |
0.4571 |
Tier III |
| 8 |
NC_000013.11 |
13 |
34683128 |
+ |
LOC105370159 |
NNNAAATACAAGAACATCCC |
NGG |
2 |
0.4286 |
Tier III |
| 9 |
NC_000008.11 |
8 |
80183123 |
+ |
LOC105375920 |
NNNATATTCTAGAACATTCC |
NGG |
2 |
0.2755 |
Tier III |
| 10 |
NC_000001.11 |
1 |
195591957 |
+ |
LOC105371671 |
NNNATATACTAGAAAATCCC |
NGT |
2 |
0.0036 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000068.7 |
2 |
93298236 |
+ |
Tspan18 |
NNNATATACCAGAACATCCC |
NGG |
1 |
0.5333 |
Tier II |
| 2 |
NC_000078.6 |
12 |
34526904 |
- |
Hdac9 |
NNNACATACTAGAACATCCA |
NGG |
2 |
0.5 |
Tier II |
| 3 |
NC_000069.6 |
3 |
10367831 |
- |
Chmp4c |
NNNATAAACCAGAACATCCC |
NGG |
2 |
0.4667 |
Tier II |
| 4 |
NC_000077.6 |
11 |
110064902 |
+ |
Abca8a |
NNNATCTAATAGAACATCCC |
NGG |
2 |
0.3896 |
Tier II |
| 5 |
NC_000071.6 |
5 |
115882005 |
+ |
Cit |
NNNATATCTTAGAACATCCC |
NGG |
2 |
0.375 |
Tier II |
| 6 |
NC_000077.6 |
11 |
89722875 |
+ |
Ankfn1 |
NNNATATTCTAGAACATTCC |
NGG |
2 |
0.2755 |
Tier II |
| 7 |
NC_000071.6 |
5 |
52840649 |
- |
Anapc4 |
NNNAGATCCTAGAACATCCC |
NGG |
2 |
0.2743 |
Tier II |
| 8 |
NC_000080.6 |
14 |
60505430 |
+ |
Spata13 |
NNNATATTCTACAACATCCC |
NGG |
2 |
0.2269 |
Tier II |
| 9 |
NC_000082.6 |
16 |
96698115 |
- |
Dscam |
NNNATATACTAACACATCCC |
NGG |
2 |
0.1965 |
Tier II |
| 10 |
NC_000067.6 |
1 |
150367417 |
- |
Odr4 |
NNNATATACTAGACCATCCC |
NAG |
2 |
0.0556 |
Tier II |
| 11 |
NC_000075.6 |
9 |
41899855 |
+ |
Gm40513 |
NNNATATAATAAAACATCCC |
NGG |
2 |
0.8 |
Tier III |
| 12 |
NC_000080.6 |
14 |
54218614 |
+ |
Tcra |
NNNAAATACTAGAACGTCCC |
NGG |
2 |
0.0962 |
Tier III |
| 13 |
NC_000072.6 |
6 |
68865908 |
+ |
Igk |
NNNATATACTATACCATCCC |
NGG |
2 |
0.0824 |
Tier III |
| 14 |
NC_000067.6 |
1 |
90903357 |
- |
Gm35381 |
NNNATACACTAGAACCTCCC |
NGG |
2 |
0.0 |
Tier III |
Other clones with same target sequence:
(none)
