Construct: sgRNA BRDN0001147858
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CATGTGGAATACTACCAAGA
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- NTRK3 (4916)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76181
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000015.10 |
15 |
88135244 |
- |
NTRK3 |
NNNGTGGAATACTACCAAGA |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000009.12 |
9 |
34787871 |
- |
PHF24 |
NNNGTGGAATATTACCAAGG |
NGG |
2 |
0.4118 |
Tier II |
| 3 |
NC_000010.11 |
10 |
103597743 |
- |
SH3PXD2A |
NNNGTGCAATATTACCAAGA |
NGG |
2 |
0.3702 |
Tier II |
| 4 |
NC_000008.11 |
8 |
15549833 |
+ |
TUSC3 |
NNNGTTGAATACTACCATGA |
NGG |
2 |
0.3333 |
Tier II |
| 5 |
NC_000002.12 |
2 |
30481598 |
+ |
LCLAT1 |
NNNGTGGAATGCTGCCAAGA |
NGG |
2 |
0.3033 |
Tier II |
| 6 |
NC_000009.12 |
9 |
105413809 |
+ |
SLC44A1 |
NNNCTGGAACACTACCAAGA |
NGG |
2 |
0.2824 |
Tier II |
| 7 |
NC_000017.11 |
17 |
7652407 |
+ |
ATP1B2 |
NNNGTGGAATTCTTCCAAGA |
NGG |
2 |
0.1641 |
Tier II |
| 8 |
NC_000011.10 |
11 |
120603381 |
- |
GRIK4 |
NNNGTGGAATACACCCAAGA |
NGG |
2 |
0.1484 |
Tier II |
| 9 |
NC_000023.11 |
X |
68053496 |
+ |
OPHN1 |
NNNGTGGAATACTCCCAACA |
NGG |
2 |
0.0961 |
Tier II |
| 10 |
NC_000006.12 |
6 |
35317667 |
+ |
DEF6 |
NNNGTGGAATAATAGCAAGA |
NGG |
2 |
0.0357 |
Tier II |
| 11 |
NC_000005.10 |
5 |
75312210 |
+ |
LOC124901007 |
NNNATGGAATACTACAAAGA |
NGG |
2 |
0.9 |
Tier III |
| 12 |
NC_000013.11 |
13 |
54805312 |
- |
LOC105370213 |
NNNCTGGAACACTACCAAGA |
NGG |
2 |
0.2824 |
Tier III |
| 13 |
NC_000008.11 |
8 |
85887025 |
- |
LOC124901971 |
NNNGTGGAAAACTACCAAGA |
NAG |
2 |
0.2222 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000075.6 |
9 |
14781407 |
- |
Ankrd49 |
NNNGTGGAATAATACCAAGG |
NGG |
2 |
0.5462 |
Tier I |
| 2 |
NC_000086.7 |
X |
137773273 |
+ |
Il1rapl2 |
NNNATGGAATACTACAAAGA |
NGG |
2 |
0.9 |
Tier II |
| 3 |
NC_000081.6 |
15 |
64225742 |
- |
Asap1 |
NNNGTGAAGTACTACCAAGA |
NGG |
2 |
0.6667 |
Tier II |
| 4 |
NC_000076.6 |
10 |
43020242 |
- |
Sobp |
NNNGTAGCATACTACCAAGA |
NGG |
2 |
0.4286 |
Tier II |
| 5 |
NC_000085.6 |
19 |
47263363 |
- |
Sh3pxd2a |
NNNGTGCAATATTACCAAGA |
NGG |
2 |
0.3702 |
Tier II |
| 6 |
NC_000073.6 |
7 |
119581968 |
- |
Acsm2 |
NNNGGGGAACACTACCAAGA |
NGG |
2 |
0.3413 |
Tier II |
| 7 |
NC_000076.6 |
10 |
106577667 |
- |
Ppfia2 |
NNNGAGGACTACTACCAAGA |
NGG |
2 |
0.2857 |
Tier II |
| 8 |
NC_000076.6 |
10 |
44301256 |
+ |
Atg5 |
NNNGTGGAATGCTACTAAGA |
NGG |
2 |
0.2 |
Tier II |
| 9 |
NC_000075.6 |
9 |
109824258 |
+ |
Spink8 |
NNNGTTGAATACTACCAAGA |
NAG |
2 |
0.1728 |
Tier II |
| 10 |
NC_000083.6 |
17 |
49470666 |
- |
Daam2 |
NNNGTGGAATACTACTAAGC |
NGG |
2 |
0.0699 |
Tier II |
| 11 |
NC_000071.6 |
5 |
19629333 |
+ |
Magi2 |
NNNGTGGAATACTACCAAGA |
NCC |
2 |
0.0 |
Tier II |
| 12 |
NC_000076.6 |
10 |
106577667 |
- |
4930532I03Rik |
NNNGAGGACTACTACCAAGA |
NGG |
2 |
0.2857 |
Tier III |
Other clones with same target sequence:
(none)
