Construct: sgRNA BRDN0001147859
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- CGGGGCTTTCAAATTCGAAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- PANK1 (53354)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76559
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000010.11 |
10 |
89611713 |
- |
PANK1 |
NNNGGCTTTCAAATTCGAAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000017.11 |
17 |
63407817 |
+ |
TANC2 |
NNNGACTTTGAAATTCGAAG |
NGG |
2 |
0.337 |
Tier II |
| 3 |
NC_000010.11 |
10 |
84478659 |
+ |
CCSER2 |
NNNGGCTTTCAAACTAGAAG |
NGG |
2 |
0.2857 |
Tier II |
| 4 |
NC_000001.11 |
1 |
25564796 |
- |
LDLRAP1 |
NNNGGCTTTCAAATTCCAAA |
NGG |
2 |
0.2206 |
Tier II |
| 5 |
NC_000011.10 |
11 |
6568613 |
+ |
DNHD1 |
NNNGGCTCTCAAATTGGAAG |
NGG |
2 |
0.1538 |
Tier II |
| 6 |
NC_000007.14 |
7 |
134883338 |
+ |
CALD1 |
NNNGGCTTTCAAATTTGAAG |
NGA |
2 |
0.0214 |
Tier II |
| 7 |
NC_000006.12 |
6 |
9845581 |
- |
OFCC1 |
NNNGGATTTGAAATTCGAAG |
NGG |
2 |
0.3611 |
Tier III |
| 8 |
NC_000017.11 |
17 |
63407817 |
+ |
LOC105371856 |
NNNGACTTTGAAATTCGAAG |
NGG |
2 |
0.337 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000080.6 |
14 |
65250626 |
+ |
Fzd3 |
NNNGGCTATCAAATTCAAAG |
NGG |
2 |
0.7467 |
Tier II |
| 2 |
NC_000086.7 |
X |
26528463 |
+ |
Slx |
NNNGGATTTCAAATTCCAAG |
NGG |
2 |
0.2185 |
Tier II |
| 3 |
NC_000084.6 |
18 |
15617486 |
+ |
Chst9 |
NNNGCCTTTCAAATTCTAAG |
NGG |
2 |
0.1964 |
Tier II |
| 4 |
NC_000078.6 |
12 |
75826711 |
- |
Syne2 |
NNNGCCTTTCAAATTCGAAG |
NGT |
2 |
0.0127 |
Tier II |
| 5 |
NC_000078.6 |
12 |
74915410 |
- |
Kcnh5 |
NNNGGCTTTCAAATTGGAAG |
NGA |
2 |
0.0107 |
Tier II |
| 6 |
NC_000083.6 |
17 |
73507748 |
- |
Galnt14 |
NNNGGCTTTCAAATTCGTAG |
NGT |
2 |
0.0081 |
Tier II |
| 7 |
NC_000086.7 |
X |
25364793 |
+ |
Gm1989 |
NNNGGATTTCAAATTCCAAG |
NGG |
2 |
0.2185 |
Tier III |
| 8 |
NC_000084.6 |
18 |
15617486 |
+ |
Gm30452 |
NNNGCCTTTCAAATTCTAAG |
NGG |
2 |
0.1964 |
Tier III |
Other clones with same target sequence:
(none)
