Construct: sgRNA BRDN0001147868
Construct Description:
- Construct Type:
- sgRNA
- Other Identifiers:
- n/a
- DNA Barcode:
- ACTGGGCGAATGTATTTGAG
Original Target:
- Taxon:
-
Homo sapiens (human)
- Gene:
- HIPK2 (28996)
- Gene Description:
- n/a
- CRISPR Mechanism
- CRISPRko
Vector Information:
- Vector Backbone:
- pXPR_003
- Pol II Cassette 1:
- EF1A-PuroR
- Pol II Cassette 2:
- n/a
- Selection Marker:
- PuroR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Additional Information:
- Addgene ID:
- 76752
Gene matches for this target sequence (CRISPRko, PAM=NGG, human):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000007.14 |
7 |
139716141 |
+ |
HIPK2 |
NNNGGGCGAATGTATTTGAG |
NGG |
0 |
1.0 |
Tier I |
| 2 |
NC_000020.11 |
20 |
19573198 |
- |
SLC24A3 |
NNNGGGCAAATGTATTTGAA |
NGG |
2 |
0.9375 |
Tier II |
| 3 |
NC_000015.10 |
15 |
67173836 |
+ |
SMAD3 |
NNNGGGAGAATGCATTTGAG |
NGG |
2 |
0.5921 |
Tier II |
| 4 |
NC_000012.12 |
12 |
49397417 |
+ |
SPATS2 |
NNNGGGTGCATGTATTTGAG |
NGG |
2 |
0.4643 |
Tier II |
| 5 |
NC_000001.11 |
1 |
101011547 |
- |
DPH5 |
NNNGGGTGAAGGTATTTGAG |
NGG |
2 |
0.325 |
Tier II |
| 6 |
NC_000017.11 |
17 |
7102714 |
- |
ASGR2 |
NNNGGGCCAATGTACTTGAG |
NGG |
2 |
0.1678 |
Tier II |
| 7 |
NC_000020.11 |
20 |
32885876 |
- |
EFCAB8 |
NNNGGGCTAATGGATTTGAG |
NGG |
2 |
0.163 |
Tier II |
| 8 |
NC_000003.12 |
3 |
99982981 |
+ |
FILIP1L |
NNNGGGCGAATGTCTTTGAG |
NTG |
2 |
0.0083 |
Tier II |
| 9 |
NC_000003.12 |
3 |
99982981 |
+ |
CMSS1 |
NNNGGGCGAATGTCTTTGAG |
NTG |
2 |
0.0083 |
Tier II |
| 10 |
NC_000003.12 |
3 |
99982981 |
+ |
LOC105374010 |
NNNGGGCGAATGTCTTTGAG |
NTG |
2 |
0.0083 |
Tier III |
Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):
| Reference Sequence |
Chromosome |
Cut Position |
Match Strand |
Match Gene |
Match Sequence |
PAM Seq. |
Mismatches[?]Genomic matches are found by querying with a reduced sequence corresponding to the final 17mer of the 20mer guide target sequence, combined with the `GG` of the PAM sequence. Mismatches are assessed within this set of bases only, for a total of 19 possible matching bases. |
CFD Score[?]Off target matches are assesed via a Cutting Frequency Determination, or CFD score. This value provides an estimate of how specific mismatches affect the likelihood that an sgRNA will cut a given target sequence. For more information, see Doench, Fusi et al., Nature Biotechnology 2016. |
Match Tier[?]For a description of CRISPR match tiers, see How the sgRNA Designer Works. |
| 1 |
NC_000072.6 |
6 |
38818439 |
+ |
Hipk2 |
NNNGGGCGAATATACTTGAG |
NGG |
2 |
0.2545 |
Tier I |
| 2 |
NC_000072.6 |
6 |
128360741 |
- |
Rhno1 |
NNNGGACGAATGCATTTGAG |
NGG |
2 |
0.7895 |
Tier II |
| 3 |
NC_000073.6 |
7 |
124320959 |
+ |
Hs3st4 |
NNNGGAAGAATGTATTTGAG |
NGG |
2 |
0.75 |
Tier II |
| 4 |
NC_000070.6 |
4 |
148565876 |
+ |
Exosc10 |
NNNAGGAGAATGTATTTGAG |
NGG |
2 |
0.675 |
Tier II |
| 5 |
NC_000079.6 |
13 |
110177766 |
+ |
Rab3c |
NNNTGGGGAATGTATTTGAG |
NGG |
2 |
0.1711 |
Tier II |
Other clones with same target sequence:
(none)
