Construct: sgRNA BRDN0001147869

Construct Description:

Construct Type:
sgRNA
Other Identifiers:
n/a
DNA Barcode:
GGCATGGTGGCAATTCTCAT

Original Target:

Taxon:
Homo sapiens (human)
Gene:
SGK1 (6446)
Gene Description:
n/a
CRISPR Mechanism
CRISPRko

Vector Information:

Vector Backbone:
pXPR_003
Pol II Cassette 1:
EF1A-PuroR
Pol II Cassette 2:
n/a
Selection Marker:
PuroR
Visible Reporter:
n/a
Epitope Tag:
n/a

Additional Information:

Addgene ID:
77458

Gene matches for this target sequence (CRISPRko, PAM=NGG, human):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000006.12 6 134174737 - SGK1 NNNATGGTGGCAATTCTCAT NGG 0 1.0 Tier I
2 NC_000022.11 22 24084699 - CABIN1 NNNATGGTGGCAATTCTCTT NAG 2 0.1396 Tier I
3 NC_000013.11 13 101737269 + FGF14 NNNATGGTGGCGATTATCAT NGG 2 0.7222 Tier II
4 NC_000003.12 3 16385886 - RFTN1 NNNATGATGGCAGTTCTCAT NGG 2 0.6522 Tier II
5 NC_000002.12 2 149041933 + LYPD6B NNNATGGTGGCAATTCTCAA NGG 1 0.5625 Tier II
6 NC_000006.12 6 99309561 - FAXC NNNATGCTGGCAATTCTTAT NGG 2 0.442 Tier II
7 NC_000001.11 1 197371368 + CRB1 NNNATGGTGCCAAATCTCAT NGG 2 0.2476 Tier II
8 NC_000004.12 4 109732332 - CFI NNNATGGTGGTGATTCTCAT NGG 2 0.2222 Tier II
9 NC_000020.11 20 44341164 + R3HDML NNNATGGTGGCAATTCCCCT NGG 2 0.146 Tier II
10 NC_000001.11 1 41792995 - HIVEP3 NNNATGGTGGCCATTCACAT NGG 2 0.1404 Tier II
11 NC_000012.12 12 65835034 - HMGA2 NNNATGGTCGGAATTCTCAT NGG 2 0.1346 Tier II
12 NC_000004.12 4 129007951 - SCLT1 NNNAGGGTGGCAATTCTCAG NGG 2 0.1129 Tier II
13 NC_000001.11 1 16143717 + EPHA2 NNNATGGTGGCAATTCACCT NGG 2 0.1103 Tier II
14 NC_000004.12 4 145826440 - ZNF827 NNNATGGTGGCTACTCTCAT NGG 2 0.0952 Tier II
15 NC_000017.11 17 6084182 + WSCD1 NNNATGGTGGCAATTCTGAA NGG 2 0.075 Tier II
16 NC_000023.11 X 36069100 + CFAP47 NNNATGATGGCAATTCTCAT NTG 2 0.039 Tier II
17 NC_000002.12 2 127573054 + MYO7B NNNATGGTGGCACTTCTCAG NGG 2 0.0372 Tier II
18 NC_000008.11 8 135473920 - KHDRBS3 NNNATGGTGGCCATTCTCAT NGA 2 0.0183 Tier II
19 NC_000010.11 10 129867498 - EBF3 NNNATGGTGGCAATTTTCAT NTG 2 0.012 Tier II
20 NC_000009.12 9 128066367 + NAIF1 NNNATGGTGGCAATGCTCAG NGG 2 0.0088 Tier II
21 NC_000001.11 1 72496265 - LOC105378797 NNNATGGTGACAATTCTCAA NGG 2 0.525 Tier III
22 NC_000014.9 14 97745415 - LOC105370651 NNNATGGTCGCAATTCTAAT NGG 2 0.2899 Tier III
23 NC_000004.12 4 54789941 - LOC105377657 NNNTTGGTGGCAATTCTCAG NGG 2 0.1123 Tier III
24 NC_000002.12 2 117903134 + HTR5BP NNNATGGTGGGAATTCTGAT NGG 2 0.0333 Tier III
25 NC_000005.10 5 38699656 + OSMR-DT NNNATGCTGGCAATTCTCAT NGC 2 0.0153 Tier III
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Gene matches for this target sequence (CRISPRko, PAM=NGG, mouse):

Reference Sequence Chromosome Cut Position Match Strand Match Gene Match Sequence PAM Seq. Mismatches[?] CFD Score[?] Match Tier[?]
1 NC_000076.6 10 21994821 + Sgk1 NNNATGGTAGCGATTCTCAT NGG 2 0.4643 Tier I
2 NC_000084.6 18 12213478 + Npc1 NNNTTGGTGGCATTTCTCAT NGG 2 0.1909 Tier I
3 NC_000068.7 2 26733797 + Gm39773 NNNATGATGGCAATGCTCAT NGG 2 0.05 Tier I
4 NC_000068.7 2 181992675 + Gm14496 NNNATAGTGGAAATTCTCAT NGG 2 0.75 Tier II
5 NC_000071.6 5 108807258 - Vmn2r8 NNNATAGTGGAAATTCTCAT NGG 2 0.75 Tier II
6 NC_000071.6 5 109005259 - Vmn2r10 NNNATAGTGGAAATTCTCAT NGG 2 0.75 Tier II
7 NC_000068.7 2 71890667 + Pdk1 NNNATAGTGGCAGTTCTCAT NGG 2 0.6522 Tier II
8 NC_000067.6 1 180258928 - Psen2 NNNATAGTGGCACTTCTCAT NGG 2 0.2105 Tier II
9 NC_000068.7 2 145197323 + Slc24a3 NNNATAGTGGCACTTCTCAT NGG 2 0.2105 Tier II
10 NC_000077.6 11 54066443 + Pdlim4 NNNATGCTGGCATTTCTCAT NGG 2 0.2063 Tier II
11 NC_000073.6 7 91140974 + Dlg2 NNNTTGGTGGTAATTCTCAT NGG 2 0.1958 Tier II
12 NC_000073.6 7 19551013 + Ppp1r37 NNNATGGTGGCAATCCTCAT NAG 2 0.0707 Tier II
13 NC_000072.6 6 49442331 - Stk31 NNNCTGGTGGCAATTCTCAG NGG 2 0.0623 Tier II
14 NC_000072.6 6 22254236 + Cped1 NNNATGGTGGCGATTCTCAT NGA 2 0.0502 Tier II
15 NC_000080.6 14 124596060 - Fgf14 NNNATGGTGACAATGCTCAT NGG 2 0.0467 Tier II
16 NC_000074.6 8 32030208 + Nrg1 NNNATGGTGGCCATTCTGAT NGG 2 0.0351 Tier II
17 NC_000069.6 3 159877044 + Wls NNNATGTTGGCAATTCTCAT NTG 2 0.0223 Tier II
18 NC_000084.6 18 23567700 - Dtna NNNAAGGTGGCAATTCTCAT NGT 2 0.0081 Tier II
19 NC_000079.6 13 65365561 - Vmn2r-ps95 NNNATTGTGGAAATTCTCAT NGG 2 0.5 Tier III
20 NC_000079.6 13 65433957 - Vmn2r-ps96 NNNATTGTGGAAATTCTCAT NGG 2 0.5 Tier III
21 NC_000079.6 13 65433957 - Gm40985 NNNATTGTGGAAATTCTCAT NGG 2 0.5 Tier III
22 NC_000079.6 13 65576480 - Vmn2r-ps97 NNNATTGTGGAAATTCTCAT NGG 2 0.5 Tier III
23 NC_000079.6 13 65877396 + Vmn2r-ps99 NNNATTGTGGAAATTCTCAT NGG 2 0.5 Tier III
24 NC_000067.6 1 180258928 - Gm31728 NNNATAGTGGCACTTCTCAT NGG 2 0.2105 Tier III
25 NC_000068.7 2 102950192 - Gm34483 NNNATGGGGGCCATTCTCAT NGG 2 0.193 Tier III
26 NC_000078.6 12 115165352 - Igh NNNATGGTGGCCATTCTCTT NGG 2 0.1417 Tier III
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Other clones with same target sequence:

(none)